Microorganisms of thePleuropneumonia group (family ofMycoplasmataceae) in man

Ag, Huijsmans-Evers; Ac, Ruys

doi:10.1007/bf02538351

Cited by 31 publications

(4 citation statements)

References 5 publications

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“…In order to type mycoplasma strains this technique was modified by several workers. Huijsmans-Evers & Ruys (1956) placed filter-paper disks soaked in specific antisera on the agar surface. Herderschee, Ruys & van Rhijn (1963) used drops of antisera on the agar and Clyde (1964) evaluated and standardized the filter-paperdisk method.…”

Section: (Received 22 October 1965)mentioning

confidence: 99%

“…The same sera, however, did not produce heterotypic growth inhibition of the organisms used in the present study; the test we describe therefore appears to be extremely specific. The growth-inhibition technique employing filter-paper disks soaked in antiserum (Huijsmans-Evers et al 1956;Clyde, 1964) is also specific but not applicable to the quantitative measurement of antibody because of its low sensitivity. Although the FI technique has a less generalized application, since it can only be used for acid-producing mycoplasmas, it appears to be at least as sensitive as other serological techniques.…”

mentioning

confidence: 99%

“…Secondly, the test may be used as a tool in epidemiological studies. In this connexion, the occurrence of antibody to M. fermentans in 13-5 % of adult sera diluted 1/5 is of interest since reports of the isolation of this mycoplasma species from man are few (Huijsmans-Evers & Ruys, 1956;Ruiter & Wentholt, 1953). Recently, 'virus-like' particles associated with human leukaemic bone marrow (Murphy, Furtado & Plata, 1965) have been identified as M. fermentans in this laboratory.…”

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confidence: 99%

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A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Taylor‐Robinson

Purcell

Wong

et al. 1966

J. Hyg.

293

119

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A property of mycoplasmas (PPLO) is the inhibition of their growth by specific antibody. Edward & Fitzgerald (1954) Jensen (1964). This is based upon the inhibition of reduction of tetrazolium. Unfortunately, this technique does not have wide application since M. pneumoniae is unique among the mycoplasma species that infect man in its ability to reduce readily tetrazolium salts. However, the growth of M. pneumoniae and certain other mycoplasma species is accompanied by the production of acid, and the incorporation of phenol red into the medium permits the resultant pH change to be seen. The addition of specific antiserum to the medium inhibits mycoplasma growth and this inhibition can be visualized by failure of the indicator to change colour. This communication describes the development of this technique for the quantitative measurement of growth-inhibiting antibody to the acidproducing mycoplasmas and indicates its practical significance.

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Section: (Received 22 October 1965)mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Taylor‐Robinson

Purcell

Wong

et al. 1966

J. Hyg.

293

119

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“…Growth inhibition tests were performed, as described previously (2,8), by applying antiserumimpregnated filter-paper discs to the surface of agar medium, uniformly inoculated with a suspension of mycoplasmas. The agar medium was incubated for 2 to 5 days and then examined with a dissecting microscope for zones of growth inhibition surrounding the paper discs.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Newly Identified Mycoplasma (Mycoplasma oraleType 3) from the Human Oropharynx

1969

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Six mycoplasma strains, isolated under anaerobic conditions from the human oropharynx, were studied by biologic and serologic means. The strains produced nippled colonies with weak hemolytic activity for guinea pig erythrocytes on agar medium. In addition, the strains metabolized arginine with a concomitant alkaline shift in the pH of the medium but did not produce a pH shift when grown in the presence of glucose or urea. The strains failed to reduce 2-3-5 triphenyl tetrazolium and were inhibited by 0.001% methylene blue. In addition, they required fresh yeast extract for growth. When compared by several serologic methods, the strains were found to be related to each other but distinct from 23 serotypes of human, animal, and avian origin. However, one-way serologic relationships between one of the new strains and Mycoplasma orale type 1 and M. salivarium were observed when they were tested by complement fixation. Furthermore, partial relationship of one of the new strains to all of the arginine-utilizing mycoplasma species of human origin was demonstrated with the agar gel diffusion technique. Thus, the new strains appear to constitute a new mycoplasma species, for which the name M. orale type 3 is tentatively proposed. M. orale type 3 accounted for 1.4% of 437 mycoplasma isolates from the oropharynx of adults. The new species probably is a rare member of the normal mycoplasmal flora of man.

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Serological Identification of Mollicutes

Tully

1993

Rapid Diagnosis of Mycoplasmas

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Microorganisms of thePleuropneumonia group (family ofMycoplasmataceae) in man

Cited by 31 publications

References 5 publications

A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Characterization of a Newly Identified Mycoplasma (Mycoplasma oraleType 3) from the Human Oropharynx

Serological Identification of Mollicutes

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