Micropatterned designs of thermoresponsive surfaces for modulating cell behaviors

Abstract

The chemistry and topography of the material surfaces have an important effect on cell behaviors. In this study, we reported the preparation of thermoresponsive micropatterned surfaces (TS) and galactosylated TS for modulating the adhesion/detachment of cells. A thickness of 1 μm of poly(N-isopropylacrylamide) grafted layer was fabricated on the polystyrene surface with microgrooves using ultraviolet-induced copolymerization. The thick grafted layer was in favor of the interactions between cells and materials. The following immobilization of galactose ligand with specific affinity to hepatocyte onto TS promoted the adhesion of human hepatocyte line (HL-7702 cells). The microgrooves structure could facilitate cell adhesion and regulate the oriented growth of cells. Moreover, narrow grooves accelerated the spontaneous detachment of cells only by reducing temperature. Thus, micropatterned biofunctional designs with controlled geometrical features presented in this study have sufficient biofunctional activities in facilitating cell sheet engineering and regenerative medicine.

Enzyme‐free cell detachment mediated by resonance vibration with temperature modulation

“…Surface modification of cell culture base is standard tactic for cell detachment without enzyme. One approach is based on temperature‐responsive polymer (Nakajima et al, ; Yang et al, ). Seeded cells adhere to the substrate and proliferate at 37°C.…”

“…Surface modification of cell culture base is standard tactic for cell detachment without enzyme. One approach is based on temperature-responsive polymer (Nakajima et al, 2001;Yang et al, 2013). Seeded cells adhere to the substrate and proliferate at 37 C. When the temperature is lowered, the surface of the cell culture base is rapidly hydrated and spontaneously releases the cultured cells without enzyme treatment.…”

Micropatterned fabrication of chitosan-based thermoresponsive membranes for improving cell adhesion and gene expression

“…In addition, the introduction of biocompatible chitosan also improved the proliferation of cells. In our prior study, 38 galactose was immobilized on the micropatterned PS surface with microgrooves by UV photografting to improve the biocompatiblity of substrate. However, the amount of galactose grafted on the surface of PS was low; therefore, the biocompatiblity of substrate was slightly increased.…”

A Substrate for Stem Cell Culture and Differentiation

“…At the moment, it is believed that stem cells can sense and initiate an appropriate response to the physicochemical properties of their extracellular matrix (ECM) or any substrate that they are cultured on, by regulating their complex signaling pathways (11)(12)(13)(14)(15)(16). Cells are naturally located in a chemically and topographically complicated environment in vivo, which is different from what is used commonly for in vitro culture conditions (17)(18)(19). Several sizes of topographies, from macro to micro, and nano scale featured cells might be faced (20).…”