Micropatterning and nanopatterning with polymeric materials for advanced biointerface‐controlled systems

Li, Ping; Dou, Xiaoqiu; Schönherr, Holger

doi:10.1002/pi.5770

Cited by 10 publications

(11 citation statements)

References 90 publications

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“…However, not only the aforementioned variables but also the geometrical distribution of ECM ligands play a role in the cell behaviour. As the techniques to pattern ECM proteins on substrates improve ( Li et al, 2019 ), it has been possible to show that the geometry of ligand distribution is essential for cell growth ( Théry et al, 2006 ) and differentiation ( Wang et al, 2019 ). Moreover, surface patterning represents a different route to vary ligand density on the substrate surface in alternative, or addition, to ECM protein dilution.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins

Lemma

Jiang

Klein

et al. 2022

Front. Bioeng. Biotechnol.

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Cells mechanical behaviour in physiological environments is mediated by interactions with the extracellular matrix (ECM). In particular, cells can adapt their shape according to the availability of ECM proteins, e.g., fibronectin (FN). Several in vitro experiments usually simulate the ECM by functionalizing the surfaces on which cells grow with FN. However, the mechanisms underlying cell spreading on non-uniformly FN-coated two-dimensional substrates are not clarified yet. In this work, we studied cell spreading on variously functionalized substrates: FN was either uniformly distributed or selectively patterned on flat surfaces, to show that A549, BRL, B16 and NIH 3T3 cell lines are able to sense the overall FN binding sites independently of their spatial arrangement. Instead, only the total amount of available FN influences cells spreading area, which positively correlates to the FN density. Immunocytochemical analysis showed that β1 integrin subunits are mainly responsible for this behaviour, as further confirmed by spreading experiments with β1-deficient cells. In the latter case, indeed, cells areas do not show a dependency on the amount of available FN on the substrates. Therefore, we envision for β1 a predominant role in cells for sensing the number of ECM ligands with respect to other focal adhesion proteins.

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Section: Introductionmentioning

confidence: 99%

Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins

Lemma

Jiang

Klein

et al. 2022

Front. Bioeng. Biotechnol.

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“…inflammation and scar tissue formation around the metal–tissue interface) that motivates the development of softer non‐degradable conductive coatings (e.g. conductive polymers), 32–39 potentially using microscale/nanoscale patterns to instruct cell behaviour, 40,41 or indeed capable of delivering bioactive substances from the electrode coating (e.g. anti‐inflammatories such as dexamethasone phosphate (DMP) and antimicrobials) 42 …”

Section: Introductionmentioning

confidence: 99%

Wirelessly triggered bioactive molecule delivery from degradable electroactive polymer films

Ashton

Appen

Fırlak

et al. 2020

Polymer International

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The development of stimuli‐responsive drug delivery systems offers significant opportunities for innovations in industry. It is possible to produce polymer‐based drug delivery devices enabling spatiotemporal control of the release of the drug triggered by an electrical stimulus. Here we describe the development of a wireless controller for drug delivery from conductive/electroactive polymer‐based biomaterials and demonstrate its function in vitro. The wireless polymer conduction controller device uses very low power, operating at 2.4 GHz, and has a supply voltage controller circuit which controls electrical stimulation voltage levels. The computer graphical user interface program communicates with the controller device, and it receives device information, device status and temperature data from the controller device. The prototype of the wireless controller system can trigger the delivery of a drug, dexamethasone phosphate, from a matrix of degradable electroactive polymers. Furthermore, we introduce the application of in silico toxicity screening as a potentially useful method to facilitate the design of non‐toxic degradable electroactive polymers for a multitude of biotechnological applications, addressing one of the key commercial challenges to biomaterial development, in accordance with ‘safe by design’ principles. © 2020 The Authors. Polymer International published by John Wiley & Sons Ltd on behalf of Society of Industrial Chemistry.

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“…Nanopatterning of surfaces is vital in many fields, such as in applications of semiconductors, 1 magnetic materials, 2 biointerfaces, 3 and many more. Nanopatterned interfaces have played an important role in probing biology, as the size of many biological molecules and clusters match such a scale.…”

Section: Introductionmentioning

confidence: 99%

“…Nanopatterning of surfaces is vital in many fields, such as in applications of semiconductors, magnetic materials, biointerfaces, and many more. Nanopatterned interfaces have played an important role in probing biology, as the size of many biological molecules and clusters match such a scale. , For example, integrins, used by cells to bind to (and transduce information from) the extracellular matrix, are in the size scale of 8–12 nm , and the universal length scale for integrin clustering and activation has been suggested to be between 58 and 73 nm .…”

Section: Introductionmentioning

confidence: 99%

Polystyrene-block-poly(ethylene oxide) Thin Films Fabricated from a Solvent Mixture for the Co-Assembly of Polymers and Proteins

Kollmetz

Gerrard

et al. 2020

ACS Omega

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The co-assembly of peptides and proteins in poly(styrene- block -ethylene oxide) (PS- b -PEO) thin films has proven to be a promising method to fabricate polymer–biomolecule functional materials. Contrary to the covalent immobilization of biomolecules on surfaces, co-assembly presents the opportunity to arrange cargo within thin films, which can be released upon exposure to an aqueous environment. The use of a mixed solvent system ensures the solubilization of hydrophobic polymer as well as the solubilization and protection of the biomolecule cargo. However, to produce largely defect-free films of PS- b -PEO from a solvent mixture containing water is challenging due to the narrow range of solvent miscibility and polymer/protein solubility. This work explores the limits of using a benzene/methanol/water solvent mixture for the production of thin PS- b -PEO films and provides a template for the fabrication optimization of block copolymer thin films in different complex solvent systems. The film quality is analyzed using optical microscopy and atomic force microscopy and correlated to the solvent composition. By adjusting the solvent composition to 80/18.8/1.2 vol % benzene/methanol/water, it was possible to reliably fabricate thin films with less than 1% macroscopic defect surface coverage. Using the optimized solvent composition, we also demonstrate the fabrication of ordered PS- b -PEO films containing lysozyme. Furthermore, we show the release of lysozyme into aqueous media, which highlights the potential use of such films for drug delivery applications.

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Micropatterning and nanopatterning with polymeric materials for advanced biointerface‐controlled systems

Cited by 10 publications

References 90 publications

Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins

Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins

Wirelessly triggered bioactive molecule delivery from degradable electroactive polymer films

Polystyrene-block-poly(ethylene oxide) Thin Films Fabricated from a Solvent Mixture for the Co-Assembly of Polymers and Proteins

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