In the last few years, the pollution of microplastics in freshwater environments such as rivers, lakes, and reservoirs has aroused widespread concerns. In this review, rich and appropriate data on microplastics, in the freshwater ecosystem of China, was collected. Following this microplastics in surface waters, sediments, and biota, of the freshwater system, were thoroughly analyzed. The results show that microplastics are widespread in the freshwater environment of China. At the same time, the abundance of microplastics is positively correlated with both intensive human activities and urbanization. The risk index of microplastics is relatively high in the water of Yellow River (654 items/L), Yangtze River (9.20 × 105 items/km2), and Pearl River regions (7571 items/m3). The prevalent shapes of microplastics, in water and sediments, are fragments and fibers. Moreover, the particle size of microplastics is mainly less than 2 mm. In fact, PP and PE are found to be the main polymer types in the freshwater environment of China. Bivalves and freshwater fish are the main research objects of microplastics pollution in China. In parallel to that, the abundance of microplastics, in each aquatic organism, varied from a few to a dozen. Additionally, the characteristics of microplastics in organisms are mainly fibers smaller than 1 mm. Fundamentally, the key two sources of microplastics, in the freshwater environment of China, are wastewater discharge and surface runoff. It is noteworthy that microplastics, in a freshwater environment, does not only cause environmental pollution but harms aquatic organisms, too. Finally, microplastics can reach the human body, through the food chain, causing potential health issues.