Micropropagation

Bhojwani, Sant S.; Dantu, Prem Kumar

doi:10.1007/978-81-322-1026-9_17

Cited by 32 publications

(22 citation statements)

References 20 publications

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“…According to another study, Jaiswal and Narayan [15] reported that the high frequency of callus induction through internode explant of F. religiosa was reached in MS medium supplemented with 0.5 mg/l NAA in combination with 1.0 mg/l BAP. It seems that 2,4-D at the high concentration may serve as an herbicide that suppressed callus formation [2]. Furthermore, our results indicated that by increasing the 2,4-D concentration, the callus formation decreased significantly.…”

Section: Resultsmentioning

confidence: 56%

“…In the other study, Parasharami et al [27] demonstrated that MS medium supplemented with 2.4 mg/l 2,4-D plus 1.0 mg/l BAP had the highest callus formation from fruit segments of F. religiosa. Ultimately, the difference results that obtained from those studies maybe due to the age, source, and the internal PGRs of explants [2], [22]. The calli that obtained from various concentrations of auxins and cytokinins possessed a friable type with yellow-brownish color (Fig.…”

Section: Resultsmentioning

confidence: 95%

“…The degree of browning is different from species to species and depend on the age of the tissue (old tissues show more browning than the younger one), the season of culture initiation (more in winters and autumn), and composition of the medium [30]. In the situation that the number of explant discoloration was high in the medium, some absorbent material such as activated charcoal (1–3 g/l) was used [2]. Thomas [42] indicated that activated charcoal could promote regeneration by absorbing inhibitory compounds and also employed for alleviating toxic metabolites, phenolic exudation, and its accumulation.…”

Section: Resultsmentioning

confidence: 99%

“…Plant tissue culture is an effective and applicable techniques for plant propagation [37]. The plantlets that derived from tissue culture are season independent, Superior, uniform, and require smaller space in comparison with the seed grown plants [2]. However, plant tissue culture had limited application in trees in comparison with other plants [34].…”

Section: Introductionmentioning

confidence: 99%

“…The age of the explant could play a significant role in plant tissue culture, and physiologically younger segments had a better performance than the older one. One way to eliminate the effect of plant’s age is to use different parts of in vitro -grown seedlings [2]. Naturally, explants from seedlings employed for standardization of in vitro propagation in plants [24], [33].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments

Hesami

Daneshvar

Yoosefzadeh-Najafabadi

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Ficus religiosa is known as a long-lived multipurpose forest tree. The tree plays an important role for religious, medicinal, and ornamental purposes. However, the propagation rate of Ficus religiosa is low in natural habitat so the plant tissue culture techniques are an applicable method for multiplication of this valuable medicinal plants. Thus, the aim of this study is to understand the effect of different auxin/cytokinin ratios on indirect shoot organogenesis of this plant. According to our results, the maximum callus induction frequency (100%) was obtained on Murashige and Skoog (MS) medium supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) plus 0.05 mg/l 6-benzylaminopurine (BAP) from petiole segments. For shoot induction purpose, the yellow-brownish, friable, organogenic calli were inoculated on shoot induction medium. On MS medium supplemented with 1.5 mg/l BAP and 0.15 mg/l Indole-3-butyric acid (IBA), 96.66% of the petiole-derived calli responded with an average number of 3.56 shoots per culture. The highest root formation frequency (96.66%), root number (5.5), and root length (4.83 cm) were achieved on MS medium containing 2.0 mg/l IBA plus 0.1 mg/l Naphthaleneacetic acid (NAA). The rooted shoots were successfully transferred to field condition and the substrate with the mixture of cocopeat and perlite (1:1) had the highest survival rate (96.66%). This is the first report of an effective in vitro organogenesis protocol for F. religiosa by indirect shoot organogenesis through axenic seedling derived petiole explants, which can be efficiently employed for conservation of this important medicinal plant species as well as the utilization of active biomolecules.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 95%