Micropropagation of apple — A review

Dobránszki, Judit; Silva, Jaime A. Teixeira da

doi:10.1016/j.biotechadv.2010.02.008

Cited by 203 publications

(171 citation statements)

References 166 publications

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“…MuiTabela 1. Resumo da análise de variância para percentagem de explantes responsivos (% ER), número de brotos (NB), número de gemas do maior broto (NG), comprimento da parte aérea (CPA) (cm) e matéria seca da parte aérea (MSPA) (mg) de plântulas de Tapirira guianensis, advindos de segmentos nodais, cotiledonares e epicótilos em diferentes concentrações de BAP (0,0; 0,5; 1,0; 1,5 e 2,0 mg L (Dobránszki & Silva, 2010). A taxa responsiva do explante segmento nodal apresentou um comportamento linear crescente, em função do aumento da concentração de BAP na ausência de ANA, podendo ser testadas concentrações acima de 2,0 mg L -1 de BAP (Figura 1A).…”

Section: Resultsunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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In vitro multiplication of Tapirira guianensis Aubl. (Anacardiaceae)Tapirira guianensis is of great medicinal, ecological and socio-economical importance occurring throughout the Brazilian territory. The objective of this study was to establish and determine the best conditions for the in vitro multiplication of T. guianensis. The explants nodal, cotyledonary and epicotyl segments from seedlings germinated in vitro were tested in diverse concentrations of 6-benzilaminopurine (BAP) and/or napthaleneacetic acid (NAA) in medium WPM. The following parameters were evaluated: the percentage of responsive explants, the number of shoots and buds, and the length of shoots and shoot dry mass at 30 and 60 days after inoculation. The cotyledonary segment, under these conditions, is the most suitable explant for the multiplication, with no induction of adventitious buds in epicotyl. The treatment with 1.0 mg L -1 BAP in the absence of NAA is the most responsive to the T. guianensis regeneration.Key words: medicinal woody, in vitro propagation, regeneration, direct organogenesis. Tapirira guianensis possui grande relevância medicinal, ecológica e socioeconômica, ocorrendo em todo o territó-rio brasileiro. O objetivo deste estudo foi estabelecer e determinar as melhores condições para a sua multiplicação in vitro. Os explantes, segmentos nodais, cotiledonares e epicótilos, oriundos de plântulas germinadas in vitro, foram testados em concentrações de 6-benzilaminopurina (BAP) e, ou, ácido naftalenoacético (ANA), em meio de cultura WPM. As características avaliadas foram a percentagem de explantes responsivos, o número de brotos e de gemas, o comprimento dos brotos e a matéria seca da parte aérea, aos 30 e 60 dias após inoculação. Foi observado que o segmento cotiledonar, nas condições deste estudo, foi o explante mais indicado para a multiplicação, não havendo indução de brotos adventícios nos epicótilos. O tratamento com 1,0 mg L -1 de BAP na ausência de ANA é o mais responsivo para a regeneração de T. guianensis.Palavras-chave: lenhosa medicinal, propagação in vitro, regeneração, organogênese direta.

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Section: Resultsunclassified

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

et al. 2013

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“…Our results showed that except for 'Redchief' with 40% shoot rooting rate, the microcuttings of other cultivars produced roots sporadically, despite of the application of the rooting media with lowered nitrate level and IBA (2.5-10 µM) supplementation, recommended by several authors as reviewed by Dobránszki and Teixeira da Silva [2010]. Apple genotypes used in our experiments are the scion cultivars of which shoots propagated in vitro are considered difficult-to-root [Lane and McDougald 1982].…”

Section: Discussionmentioning

confidence: 88%

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

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For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid -IBA, 1-naphthaleneacetic acid -NAA and indole--3-acetic acid -IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars 'Free Redstar', 'Gala Must', 'Pinova' and 'Redchief' were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60-80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.

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“…According to the review made by DOBRÁNSZKI & SILVA (2010) BIOLOGY dependence regarding the salt composition of the best propagation media. The most regular media used for apples include MS, QL, WPM and DKW; therefore, the objective of this study was to adapt a micropropagation protocol for the new apple rootstock 'G.…”

Section: Thementioning

confidence: 99%

“…According to DOBRÁNSZKI & SILVA (2010), multiplication of apple explants depends on several factors: genotype; organic and inorganic compounds and plant growth regulators, and the cytokinin BAP is used commonly in the range of 0.5 to 5.0mg L -1 (GRATTAPAGLIA & MACHADO, 1998). The authors SANTA CATARINA (2001) concluded that for apple rootstock 'Selection 69' combining 0.5mg L -1 of BAP and GA3 promoted the greatest number of shoots.…”

Section: Thementioning

confidence: 99%

Micropropagation of the new apple rootstock ‘G. 814’

et al. 2017

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International breeding programs launched new genetic material of apple rootstocks that in addition to precocity and great yield are resistant to major diseases and soil pests encountered in the largest apple producing regions in Brazil. Given this, there is a necessity for vegetative propagation of these materials for study and possible replacement of existing rootstocks. The objective was to adapt a micropropagation protocol for new apple rootstock ‘G. 814’. In the multiplication phase were evaluated BAP concentrations: 0; 0.5; 1; 2 and 4mg L-1 and in the rooting phase were evaluated IBA concentrations: 0; 0.25; 0.50; 1; 1.5 and 2.5mg L-1. These new results demonstrated that this new rootstock selection can be propagated with this tissue culture adapted protocol. For the successful in vitro propagation of apple rootstock ‘G. 814’ it is indicated the use of 1mg L-1 BAP at multiplication phase and 1.5mg L-1 IBA at rooting phase.

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Micropropagation of apple — A review

Cited by 203 publications

References 166 publications

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

Multiplicação in vitro de Tapirira guianensis Aubl. (Anacardiaceae)

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Micropropagation of the new apple rootstock ‘G. 814’

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