Micropropagation of Coleus blumei from nodal segments and shoot tips

Rani, G.; Talwar, D.; Nagpal, Avinash Kaur; Virk, G. S.

doi:10.1007/s10535-006-0078-1

Cited by 27 publications

(17 citation statements)

References 24 publications

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“…In the present investigation, MS medium augmented with 4.0 mgl -1 BAP+0.1 mgl -1 NAA found to be the best treatment for the highest multiple shoot induction as well as maximum number of shoots per explant. Similar results were also reported in several medicinal plants, such as, Smilax zeylanica L. (Sayeed Hasan and Roy, 2004); Celastrus paniculatus (Martin et al, 2006); Heracleum candicans (Wakhlu and Sharma, 1998); Spilanthes mauritiana (Bais et al, 2002); Coleus blumei (Rani et al, 2006). Table 1 Table 2) followed by 73.33% on the medium with 1.5 mgl -1 IBA.…”

Section: Resultssupporting

confidence: 82%

Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb

et al. 2013

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High frequencies of multiple shoot regeneration were achieved from shoot tip explants cultured on MS media fortified with 4.0 mgl -1 BAP + 0.1 mgl -1 NAA. On an average 10.2±0.38 shoots per explant were obtained. The regenerated shoots were rooted in MS medium supplemented with 1.0 mgl -1 IBA. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival. A reproducible protocol was established for in vitro propagation through multiple shoot induction of Centella asiatica L. an important medicinal herb having high medicinal value.

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Section: Resultssupporting

confidence: 82%

Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb

et al. 2013

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“…In vitro rooting was noticeably influenced by the concentration of the auxins as well the strength of the MS medium. Similar to our current study, rooting in MS medium supplemented with IBA has been reported to be efficient in several plants belonging to the Lamiaceae family such as Isodon wightii (Thirugnanasampandan et al, 2009), Ocimum basilicum (Siddique and Anis, 2008) and Coleus blumei (Rani et al, 2006). (Fig.…”

Section: Rooting Of Regenerated Shootssupporting

confidence: 89%

Influence of Additives on Enhanced In vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq.

JAYAKUMAR¹,

RAMALINGAM²

2013

Not Sci Biol

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Orthosiphon aristatus is a valuable medicinal plant and different parts of the plant are pharmaceutically used for the treatment of various diseases. The present study was designed to develop an efficient protocol for micropropagation of O. aristatus from nodal explants and to study the influence of additives on the enhancement of the number of shoots per explant. Among the different types of additives used, 10% coconut water and 30 mg/L glutamine added to Murashige and Skoog's (MS) medium supplemented with 1.0 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L kinetin (KIN) was found to be most effective. Maximum number of shoots (44.07 ± 0.38) with 100% shooting response and shoot length of 7.47 ± 0.10 cm was recorded. In vitro rooting of the microshoots was achieved on halfstrength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA), producing an average of 30.27 ± 0.36 roots and 6.02 ± 0.20 cm root length. The rooted shoots were acclimatized with 100% survival rate on coco pith: soil (3:1) planting substrate and was successfully transferred to field conditions. The hardened plants exhibited homogeneity and no morphological variations were observed among the regenerants and the mother plants. Thus, the procedure described is a quick and reliable method which could be applied for efficient large-scale propagation, genetic transformation assays and secondary metabolite production.

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“…There were differences between treatments in both the percentage of cultures with multiple shoots and the mean number of shoots per culture. Many authors reported that cytokinin is required in optimal quantity for shoot proliferation in many species but inclusion of a low concentration of auxin along with cytokinin increased the rate of shoot multiplication (Sharma et al 1993, Sharma and Singh 1997, Shasany et al 1998, Rout et al 2000, Rout 2005, Rani et al 2006. A higher concentration of BA (> 2.0 mg dm -3 ) in the culture medium inhibited the growth of the shoots and stimulated small callusing at the basal end.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

In vitro clonal propagation of Nyctanthes arbor-tristis

Rout¹,

Mahato²,

Senapati³

2008

Biologia plant.

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Rapid shoot multiplication of Nyctanthes arbor-tristis L. was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0 -1.5 mg dm -3 6-benzylaminopurine (BA), 50 mg dm -3 adenine sulfate (Ads) and 3 % (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg dm -3 BA, 50 mg dm -3 Ads and 0.1 mg dm -3 IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 d on half-strength MS medium supplemented with either indole-3-butyric acid (IBA), IAA or 1-naphthaleneacetic acid (NAA) with 2 % sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg dm -3 IBA and 0.1 mg dm -3 IAA. About 70 % of the rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the field.Additional key words: growth regulators, medicinal plant, shoot multiplication. ⎯⎯⎯⎯Nyctanthes arbor-tristis Linn. is a valuable medicinal plant which belongs to the family Oleaceae, and is distributed in Indo-Malayan regions. It is a small tree with scented white flowers. It is a native of India occurring wild in the Sub-Himalayan region. Due to unrestricted large-scale exploitatiobn of the natural resource, coupled with limited cultivation and insufficient attempts for its replenishment, the natural stock of this species has been markedly depleted. Propagation from seed is unreliable due to poor germination and death of many young seedlings under natural conditions (Thapliyal and Naithani 1996). In vitro culture is an alternative method for conservation and propagation of this species, but no report has been published so far. Hence, this investigation aimed to develop an efficient protocol for micropropagation of N. arbor-tristis, an important medicinal plant.Elongated shoots (4 -5 cm long) were collected from field grown plants of Nyctanthes arbor-tristis L. and brought to the laboratory with cut ends dipped in distilled water. Stem without leaves were washed in 2 % (m/v) Teepol (Qualigen, Mumbai, India) and surface disinfected by 0.1 % (m/v) aqueous mercuric chloride solution for 15 min. After rinsing 4 -5 times with sterile distilled water, stems were cut into smaller segments (ca. 0.5 cm long), each with one node was used as explant source.Nodal explants were placed on semisolid Murashige and Skoog (1962; MS) mineral salts plus 555 µM myoinositol, 4.06 µM nicotinic acid, 2.43 µM pyridoxineHCl, 0.296 µM thiamine-HCl supplemented with various concentrations of cytokinins, i.e. 6-benzylaminopurine (BA: 0.0, 0.5, 1.0, 1.5 and 2.0 mg dm -3 ), kinetin (Kn: 0.0, 0.5, 1.0, 1.5 and 2.0 mg dm -3 ), adenine sulfate (Ads: 25, 50 and 100 mg dm -3 ) and auxins like indole-3-acetic acid (IAA: 0.0, 0.10, 0.25 and 0.5 mg dm -3 ) and 1-naphthalene-acetic acid (NAA: 0.0, 0.10, 0.25 and 0.5 mg dm -3 ) for shoot multiplication. The pH of the medium was adjusted to 5.8 prior...

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Micropropagation of Coleus blumei from nodal segments and shoot tips

Cited by 27 publications

References 24 publications

Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb

Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb

Influence of Additives on Enhanced In vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq.

In vitro clonal propagation of Nyctanthes arbor-tristis

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