Micropropagation of Dendrocalamus asper by shoot proliferation using seeds

Arya, Sarita; Sharma, Sunil; Kaur, Ravneet; Arya, I. D.

doi:10.1007/s002990050678

Cited by 50 publications

(37 citation statements)

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“…Similar, observation has been reported by Hussain et al (2008) in Sterculia urens, where TDZ was found effective for direct shoot regeneration from intact seedlings. Our results are in contrast with the findings of Bhuyan et al (1997) in Murraya koenigii, Das et al (1999) in Litchi chinensis, Arya et al (1999) in Dendrocalamus asper, Hussain et al (2008) in Sterculia urens and Rani et al (2010) in Murraya Koenigii, where BA was found most effective for direct shoot regeneration from intact seedlings. At higher concentrations of TDZ (above 5.0 µM), rate of seed germination (sprouting epicotyls induction per seedling), and shoot multiplication per sprouting epicotyls decreased significantly (Fig.…”

Section: Resultscontrasting

confidence: 99%

“…The application of intact seedling method in the propagation of multipurpose plant species provides timely supply of plant materials required for the isolation of drugs by the pharmaceutical industries without depleting natural plant resources as well as for reforestation of degraded and exploited lands. Several authors (Malik et al, 1993;Prakash et al, 1994;Bhuyan et al, 1997;Arya et al, 1999;Das et al, 1999;Hussain et al, 2008) have reported the use of intact seedling method in several medicinally and economically important plant species (Lathyrus cicera, Cajanus cajan, Murraya koenigii, Dendrocalamus asper, Litchi chinensis and Sterculia urens) for quick germplasm production and conservation.…”

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confidence: 99%

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In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

Perveen¹,

Anis²,

Aref³

2013

For. syst.

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Objectives: An efficient and reproducible regeneration protocol for rapid multiplication of Albizia lebbeck (L.) was developed by using intact seed explants.Methods: Murashige and Skoog's (MS) medium supplemented with different hormones (BA, Kn, GA3 and TDZ) was used for the induction of multiple shoots from the seed explants. Ex-vitro rooting was performed by using pulse treatment method in auxins (IBA and NAA) and the complete plantlets were transferred to the field.Results: High frequency direct shoot induction was found in aseptic seed cultures of A. lebbeck on Murashige and Skoog medium supplemented with 5.0 µM TDZ (Thiadiazuron). Seeds were germinated after 7 days of culture and induced maximum 8 shoots from the region adjacent to the apex of the primary shoot of the seedling upto 25 days of incubation. Proliferating shoot cultures with increased shoot length was established by sub-culture of excised sprouting epicotyls on MS medium supplied with reduced concentrations of TDZ. Maximum shoot regeneration frequency (76 %) with highest number of shoots (21) and shoot length (5.1 cm) per sprouting epicotyl was observed in the MS medium supplemented with 0.5 µM TDZ after 8 weeks of culture. Different concentrations of Indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) were tested to determine the optimal conditions for ex-vitro rooting of the microshoots. The best treatment for maximum ex-vitro root induction frequency (81 %) was accomplished with IBA (250 µM) pulse treatment given to the basal end of the microshoots for 30 min followed by their transfer in plastic cups containing soilrite and eventually established in normal garden soil + soilrite (1:1) with 78 % survival rate. In addition, histological study was undertaken to gain a better understanding of the regenerated shoots from the epicotyl region.Conclusion: The findings will be fruitful in getting a time saving and cost effective protocol for the in vitro propagation of Albizia lebbeck.Keywords: Woody legume; TDZ; multiplication; epicotyl segment; shoot stumps.Abbreviations used: GA3 (Gibberellic acid); BA (6-Benzyladenine); Kn (Kinetin); TDZ (Thidiazuron); MS (Murashige and Skoog Medium); IBA (Indole-3-butyric acid); NAA (α-Naphthalene acetic acid).

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Section: Resultscontrasting

confidence: 99%

mentioning

confidence: 99%

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

Perveen¹,

Anis²,

Aref³

2013

For. syst.

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“…Supplementation of ADS with BAP was unable to alter / improve the frequency of response significantly, but enhanced the number of shoots and their proliferation successfully. In a sustained subculture method, on the other hand, the rate of multiplication was simultaneously increased 30 fold with the passage up to 15 cycles (subcultures) which proved to be better than the previous report of Arya et al (1999). The results demonstrated that cytokinin fruitfully promoted multiplication of shoots in an enhanced mode, in the presence of ADS, where ADS devoid of PGRs could not achieve significant result.…”

Section: Resultsmentioning

confidence: 57%

“…In the present study, semisolid MS medium with appropriate growth supplement for initial culture establishment followed by liquid medium for shoot multiplication and rooting was most suitable to optimize the in vitro mass propagation protocol, successfully. This method, particularly the alteration of semi solid to liquid MS medium, appears to be the most effective method of micropropagation of D. asper and the first complete report of micropropagation from the nodal explants, as, the other report describes use of seeds (Arya et al 1999) or rhizomes (Nirmala et al 2011) as explants. But, the species being exotic to India, the seeds are not available in this country while the plants are reported to set seed only after 100 years (Arya et al 1999).…”

Section: Resultsmentioning

confidence: 99%

“…With the view of its importance and irregular availability of the source materials, recent researchers are emphasizing on micropropagation of D. aper using different explants. Seeds (Arya et al 1999) and rhizomes (Nirmala et al 2011) were successfully used for mass propagation of this species in vitro. However, both seeds and rhizome is scarce; on the other hand, to date, no such report is available which combines both in vitro and in vivo multiplication of D. asper to enhance the rate of multiplication and evaluate the performance of the offsprings in the field.…”

Section: Introductionmentioning

confidence: 99%

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A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field

Banerjee

Gantait

Pramanik

2011

Physiol Mol Biol Plants

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An accelerated protocol for large-scale propagation of Dendrocalamus asper, an edible bamboo, has been described. Seven axillary shoots were induced in vitro from each excised tender node (15-20 mm in length) containing single axillary bud when nodal segments were inoculated in semisolid Murashige and Skoog (MS) medium fortified with 5 mg/l 6-benzylaminopurine (BAP). Maximum multiple shoot formation (14) was observed when in vitro generated axillary shoots were transferred to liquid MS medium containing 5 mg/l BAP and 40 mg/l adenine sulphate. A maximum of 93.33 % shoots were effectively rooted when transferred to liquid MS medium supplemented with 1 mg/l indole-3-biutyric acid (IBA). A simple acclimatization procedure of 55 days, primarily in cocopeat for 20 days and finally in a blend of sand, soil and farm yard manure (1:1:1 v/v), ensured a very high survival rate within next 35 days. After acclimatization, rooted plantlets were further multiplied by splitting of rhizomes, formed in vivo within 90 days of growth. After 90 and 180 days of acclimatization, plants were successfully transferred to the field and maintained in an unirrigated condition with the initial supplementation of farm yard manure @ 10 kg/pit; where around 85 % survivability with 25 culms per bush attaining an average height of 4.5 m was recorded up to four years.

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Micropropagation of Bamboo Species Through Axillary Shoot Proliferation

Jiménez

Guevara

2007

Protocols for Micropropagation of Woody Trees and Fruits

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Micropropagation of Dendrocalamus asper by shoot proliferation using seeds

Cited by 50 publications

References 10 publications

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field

Micropropagation of Bamboo Species Through Axillary Shoot Proliferation

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