Micropropagation of Gladiolus × Hybridus Hort. ‘Пермский Сувенир ’, Which Was Bred in Botanical Garden of Psu

Abstract

The article presents data on micropropagation of new Gladiolus × hybridus cultivar ‘Пермский Сувенир’. It is found that for the sterilization of corms and cormels better use the 7% sodium hypochlorite solution (15 min or 20-25 min) and then 96% ethanol (30 sec or 60 sec). Sterile culture was more than 56% for corms and 76% for cormels. At the micropropagation good results were obtained when vitamins (0.1 mg/l thiamine, 0.5 mg/l pyridoxine, 0.5 mg/l nicotinic acid) and β-indolylacetic acid (0.1-1 mg/l) were added to the Murashige and Skoog medium. The breeding rate for cormels was 2-3, for corm fragments was 2–4. The survival rate of plants transferred from in vitro to in vivo conditions was 70%. The diameter of the obtained juvenile corms from in vitro culture and the number of cormels formed on them did not have statistically significant differences from those planted in vivo (t = [1.16; 1.22] <t05 = 1.96).