Micropropagation of Kaempferia galanga L. ? a medicinal plant

Vincent, Kate; Mathew, K. M.; Hariharan, Molly

doi:10.1007/bf00055522

Cited by 57 publications

(23 citation statements)

References 8 publications

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“…The successful use of BAP for shoot production may be accredited to the capability of plant tissues to metabolize BAP more efficiently than other synthetic PGRs or to the capacity of BAP to encourage the production of natural hormones, such as zeatin, within the tissues (Malik et al, 2005). Kukreja et al (1990) and Vincent et al (1992) also reported a decline in the number of shoots with higher BAP levels. Waseem et al (2009) showed that the use of higher than optimal concentrations of PGRs may lead to poor performance.…”

Section: Effects Of Cytokinins On Shoot Developmentmentioning

confidence: 99%

Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

Abbasi

Pervaiz

Hafiz

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl 2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.

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Section: Effects Of Cytokinins On Shoot Developmentmentioning

confidence: 99%

Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

Abbasi

Pervaiz

Hafiz

et al. 2013

J. Zhejiang Univ. Sci. B

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“…There is increasing interest in using these techniques for rapid and large-scale propagation of medicinal and aromatic plants (Bajaj et al, 1988;Vincent et al, 1992;Krishnan and Seeni, 1994;Purohit et al, 1994;Sudha and Seeni, 1994;Bhat et al, 1995;Patnaik and Debata, 1996;Sahoo et al, 1997). Many in vitro studies have been conducted on Lamiaceae species, including Ocimum basilicum L. (Ahuja et al, 1982;Sahoo et al, 1997;Begum et al, 2002;, Ocimum gratissimum (Gopi et al, 2006) and Ocimum sanctum (Singh et al, 1999;Begum et al, 2000;Banu et al, 2007), but to our knowledge there are no reports on in vitro propagation of Ocimum kilimandscharicum through nodal explants.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Ocimum kilimandscharicum Guerke (Labiatae)

Saha¹,

Dey²,

Ghosh³

2010

Acta Biologica Cracoviensia Series Botanica

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An efficient plant regeneration protocol has been developed from nodal explants of Ocimum kilimandscharicum Guerke, a medicinally important herbaceous plant species belonging to the family Lamiaceae. Axillary shoot bud proliferation was initiated from nodal explants cultured on MS medium supplemented with various concentrations of 6-benzyladenine (BA) (0.5-3.0 mg/l), kinetin (KN) (0.5-3.0 mg/l) and 2-isoPentenyladenine (2-iP) (0.5-3.0 mg/l). The maximum number of shoots (6.09±0.05), with average length 3.83±0.11 cm, was achieved with medium containing 1.0 mg/l BA. Shoot culture was established by repeated subculturing of the original nodal explants on shoot multiplication medium after each harvest of newly formed shoots. In this way, 20-30 shoots were obtained from a single nodal explant after 5 months. Rooting of shoots was achieved on half-strength MS medium supplemented with 1.5 mg/1 Indole-3-butyric acid (IBA) and 2% sucrose. Well-developed plantlets transferred to plastic pots containing soil and vermiculite (1:1) showed 81.13% survival. The genetic fidelity of in-vitro-raised field-grown plants to the donor plant was ascertained from random amplified polymorphic DNA (RAPD) markers. This protocol can be used for commercial propagation and for future genetic improvement studies.K Ke ey y w wo or rd ds s: : Ocimum kilimandscharicum, nodal segments, in vitro propagation, genetic fidelity.

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“…many species (Rout et al 2002;Martins et al 2004;Venkatachalam et al 2007). The few reports so far available on tissue culture studies in K. galanga (Vincent et al 1992;Shirin et al 2000;Rahman et al 2005) do not include any work on in vitro production of extractable leaf biomass for commercial purposes and assessment of genetic stability of micropropagated plants. The present paper deals with a suitable protocol for rapid propagation of genetically uniform plants and a novel method of in vitro production of leaf biomass in K. galanga for the first time.…”

mentioning

confidence: 99%

Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture

Parida¹,

Mohanty²,

Kuanar³

et al. 2010

Electron. J. Biotechnol.

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An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of leaves after 2 months of culture under sterile condition, the base of each plantlet was transferred to fresh media which could produce the same leaf biomass within another 2 months in a 50 ml culture tube containing 20 ml and 250 ml conical flasks *Corresponding author containing 30 ml Murashige and Skoog medium. The rate of multiplication and leaf biomass production remained unchanged in subsequent subcultures. The regenerated plantlets were acclimatized in greenhouse and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 95 percent. Genetic fidelity of the regenerants was confirmed using random amplified polymorphic DNA (RAPD) marker. The protocol could be commercially utilized for large scale production of true-to-type plantlets and as an alternative method of leaf biomass production in Kaempferia galanga.Kaempferia galanga L. (K. galanga) is a rare Indian medicinal herb of family Zingiberaceae. It is distributed in the tropics and subtropics of Asia and Africa. The plant is extensively used in preparation of ayurvedic drugs, in perfumery, cosmetics and as spice ingredients (Rahman et al. 2004). The plant is used for treatment of diarrhea,

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Micropropagation of Kaempferia galanga L. ? a medicinal plant

Cited by 57 publications

References 8 publications

Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

Micropropagation of Ocimum kilimandscharicum Guerke (Labiatae)

Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture

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