Micropropagation of Karonda (Carissa carandas) through shoot multiplication

Rai, Ratna; Misra, K. K.

doi:10.1016/j.scienta.2003.09.005

Cited by 12 publications

(7 citation statements)

References 4 publications

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“…For these reasons, surface sterilization with 70% ethanol for one minute followed by 20% Clorox for 20 min was the preferred method in reducing microbial contamination of sweet shoot. This method of sterilization has been reported in tissue cultures of Jamaican yams, sugarcane and Carissa carandas (Mitchell et al, 1995;Moutia and Dookun, 1999;Rai and Misra, 2005).…”

Section: Discussionmentioning

confidence: 93%

Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

Wee¹,

Alderson²,

Yap³

2015

Asian J. of Biotechnology

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Propagation of Sauropus androgynus (SA) by tissue culture technology provides a better alternative than conventional propagation, as it ensures uniformity in production of sustainable plantlets with desirable traits. The objective was to establish an efficient plant regeneration protocol using leaf, nodal and internodal explants of SA, starting from the establishment of aseptic cultures to the acclimatization stage of plantlets. The effects of plant growth regulators on the growth performance of SA were studied. Results showed in vitro mass multiplication of SA was achieved via indirect organogenesis and shoot regeneration pathway. After 30 days of culture inoculation, high percentage of aseptic cultures were established from nodal (85%), internodal (85%) and leaf (91%) explants sterilized with 70% (v/v) ethanol for 1 min followed by 20% (v/v) Clorox for 20 min. MS medium supplemented with 2.0 mg LG 1 BAP (6-benzylaminopurine) and 0.5 mg LG 1 IAA (indole-3-acetic acid) proved to be effective in inducing adventitious shoots from internodal (95%), nodal (96%) and leaf (90%) explants, after 60 days of culture. High frequency of callus formation (38%) was obtained from leaf explants cultured on MS medium containing 2.0 mg LG 1 IAA and 0.5 mg LG 1 BAP. Rooting response of 90% was achieved from cultured shoots using half-strength MS medium enriched with 1.0 mg LG 1 IAA and from these, 85% survived acclimatization and grew vigorously after 4 weeks. This optimized protocol established a complete in vitro plant regeneration of SA and has never been reported previously. This may form the basis for future research in germplasm conservation and genetic transformation for sustainable production of high quality SA.

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Section: Discussionmentioning

confidence: 93%

Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

Wee¹,

Alderson²,

Yap³

2015

Asian J. of Biotechnology

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“…Plant growth regulators (PGRs) employed were 6-Benzylaminopurine (BAP), kinetin, 1-Naphthaleneacetic acid (NAA), Thidiazuron (TDZ), and adenine sulphate for multiple shoot induction from nodal cuttings of the wild plant. Similar attempts have been made for the propagation of Carissa carandas using shoot apex and nodal part (Hasmah, Bhatt, & Keng, 2013;Imran, Begum, Sujatha, & Mallaiah, 2012;Rai & Misra, 2005). Some reports are available on in vitro propagation of Rauvolfia serpentina (Sarker, Islam, Islam, Hoque, & Joarder, 1996) and…”

Section: Micropropagation: a Strategy For Conservation Of Apocynacementioning

confidence: 83%

Ethnopharmacology, phytochemistry, and biotechnological advances of family Apocynaceae: A review

Bhadane¹,

Patil²,

Maheshwari

et al. 2018

Phytotherapy Research

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The family Apocynaceae is one of the largest and important families in angiosperm. Several members of this family have medicinal properties and have been in the treatment of various ailments. Most of them are consumed as food by tribal people whereas a few plants are used as source of poison. Members of family Apocynaceae are rich in alkaloids, terpenoids, steroids, flavonoids, glycosides, simple phenols, lactones, and hydrocarbons. Other compounds such as sterols, lignans, sugars, lignans, and lactones have been isolated and systematically studied. Few studies have reported antioxidant, anti-inflammatory, antimicrobial, and cytotoxic activities of crude extracts as well as single compound(s) isolated from various members of the family Apocynaceae. Holarrhena antidysenterica, Rauvolfia serpentina, Carissa carandas, and Tabernaemontana divaricata are the extensively studied plants in this family. The present review provides a detailed outlook on ethnopharmacology, phytochemistry, and biological activities of selected members of this family. Moreover, it also covers the biotechnological advances used for large-scale production of bioactive compounds of therapeutic interest along with plant tissue culture-based approaches for conservation of this medicinally valuable family.

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“…Previously, 46.8% clean cultures was obtained through disinfection with Sodium Hypochlorite in experiment conducted by Moutia and Dookun (1998) and later 74.3% explants survived the culture even with the aid of ethanol, Mercuric Chloride and Sodium Hypochlorite (Rai and Misra, 2003). Present study has combined several sterilizing agents' concentration which was considered effective to develop a surface sterilization method for B. rotunda shoot bud explants.…”

Section: 30 Results and Discussionmentioning

confidence: 93%

A Non Mercuric Shoots Bud Sterilization Technique for Boesenbergia rotunda (L.) Mansf. Kulturpfl.

et al. 2013

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Boesenbergia rotunda, a medicinal herb under the Zingiberaceae family, has been proven to be the most prominent anticancer remedies. The conventional breeding of this plant is inapplicable as it is susceptible to rhizome soft rot and leaf spot diseases. The yellow rhizome also produces limited buds. Therefore it is necessary to propagate this plant through in vitro propagation to obtain abundant uniform planting materials. Unfortunately, high cost is incurred due to the high shoot bud explants contamination level. Hence, it is addressed in the present study to solve the contamination problem. Mercuric Chloride is well known to solve this problem, but it is not advisable to use, because of its poisoning and other hazardous effects. Moreover, explant sterilization technique should also accelerate the shoot response. To find an alternative, 6 different surface sterilization methods (SSM) were designed and evaluated on the explants where different combinations of sodium hypochlorite and ethanol (instead of mercuric chloride) were applied. The sterilized shoot bud explants were then cultured on Murashige & Skoog (MS) media with no additional vitamins or plant growth regulator under the light below 25oC. The contamination was recorded for 3 consecutive weeks along with visible shoot responses. SSM 5 showed minimum contamination and maximum visible shoot response, compared to other SSMs. Therefore, it is suggested that SSM5 could be used to conduct surface sterilization to avoid contamination problem.

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Micropropagation of Karonda (Carissa carandas) through shoot multiplication

Cited by 12 publications

References 4 publications

Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

Ethnopharmacology, phytochemistry, and biotechnological advances of family Apocynaceae: A review

A Non Mercuric Shoots Bud Sterilization Technique for Boesenbergia rotunda (L.) Mansf. Kulturpfl.

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