Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants

Abstract

Axillary buds from adult field-grown plants of Lavandula dentata L. were used to evaluate the effect of growth regulators and culture media on the in vitro shoot proliferation and growth. The highest multiplication rate was obtained using Murashige and Skoog (MS) medium supplemented with a combination of 2.2 µM of benzyladenine and 2.5 µM indole-3-butyric acid. The best condition for rooting was MS medium plus 2.5 µM naphthaleneacetic acid. Rooted plantlets were successfully transferred to soil. Short-term culture derived plants (6 month) exhibited a normal development, but a low frequency of not heritable morphological changes were detected in long term culture derived plants (more than 1 year).Additional key words: lavender, in vitro propagation, bud breaking.

Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation

“…Under high concentrations of BAP (4.44 and 8.88 µM), shoots were stunted due to hyperhydricity. Hyperhydricity was also a limiting factor in the propagation of other Lamiaceae species, such as Lavandula dentata (Echeverrigaray et al, 2005) and Salvia miltiorrhiza (Chen et al, 2005). As observed in other Salvia species (Cuenca andAmo-Marco, 2000, Misic et al, 2006), S. guaranitica exhibited a strong apical dominance that resulted in slow growth of axillary buds and no production of adventitious buds, consequently, a low multiplication rate.…”

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

“…Therefore, vegetative propagation is the most effective to produce a large number of uniform individuals displaying a genotype of interest. Tissue culture techniques have been applied to Lavandula plants (Quazi 1980;Calvo and Segura 1989;Jordan et al 1990;Gras and Calvo 1996;Andrade et al 1999;Echeverrigaray et al 2005;Zuzarte et al 2010;Machado et al 2011;). However, the performance and survival of in vitro cultures of many plant species are often hampered by the phenomenon of hyperhydricity (Debergh et al 1992), including lavender species.…”

A Rapid and Efficient Protocol for Clonal Propagation of Phenolic-Rich Lavandula multifida

“…The highest multiplication rate (6.14 shoots per explant) was obtained using MS supplemented with 1.0 mg/L ZEA ( Table 2). The efficiency of cytokinins was also shown on shoot multiplication of other lavenders: L. dentata (Jordan et al, 1998;Echeverrigaray et al, 2005), L. officinalis x L. latifolia (Panizza & Tognoni, 1992), L. pedunculata (Zuzarte et al, 2010), L. stoechas (Mesquita, Guimarães, & Santos Dias, 1990;Nobre, 1996) and L. vera (Andrade et al, 1999).…”

“…For instance, axillary shoot proliferation was reported for L. dentata (Jordan, Calvo, & Segura, 1998;Sudriá et al, 1999Sudriá et al, , 2001Echeverrigaray, Basso, & Andrade, 2005;Machado, Silva, & Biasi, 2012), L. latifolia (Sánchez-Gras & Calvo, 1996), L. stoechas (Nobre, 1996), L. vera (Andrade, Echeverrigaray, Fracaro, Pauletti, & Rota, 1999) and L. viridis (Dias, Almeida, & Romano, 2002;Nogueira & Romano, 2002). Direct shoot formation from different tissue explants was achieved in L. latifolia (Calvo & Segura, 1989a) and shoot formation from callus was reported for L. angustifolia (Quazi, 1980;Ghiorghita et al, 2009), L. x intermedia (Dronne, Jullien, Caissard, & Faure, 1999), L. latifolia (Calvo & Segura, 1988, 1989bJordan, Calvo, & Segura, 1990), L. officinalis x L. latifolia (Panizza & Tognoni, 1988) and L. vera (Tsuro, Koda, & Inoue, 1999, 2000.…”