Micropropagation of mature Terminalia catappa (Indian Almond), a medicinally important forest tree

Abstract

We report an efficient in vitro propagation method for Terminalia catappa using nodal segments of a 15-year-old mature tree. The nodal segments were cultured on MS medium supplemented with 6-benzyladenine (BA; 0.5-3.0 mg l -1 ) or Kinetin (Kn; 0.5-3.0 mg l -1 ) for bud breaking and multiple shoot induction. About 85% of the explant responded (2.8 ± 0.41 shoots per node with 2.7 ± 0.14 cm length) within 15 days of inoculation in Murashige and Skoog medium fortified with 2.0 mg l -1 of BA. Further shoot multiplication was achieved by repeated transfer of mother explants and subculturing of in vitroproduced shoots on medium supplemented with various concentrations of BA (0.25-1.5 mg l -1 ) or Kn (0.25-1.5 mg l -1 ) or on their combinations. Optimal number of shoots and shoot length were recorded on MS medium supplemented with 0.25 mg l -1 of BA and 0.25 mg l -1 of Kn. The multiplied shoots were used for ex vitro rooting after treatment for 4 min with indole-3-butyric acid (IBA; 50-500 mg l -1 ) or a-naphthalene acetic acid (NAA; 50-500 mg l -1 ). About 80% of the shoots treated with 200 mg l -1 of IBA produced ex vitro roots with an average of 2.8 roots per shoot. Nearly 75% of these plantlets could be acclimatized within 5 weeks and successfully established in the field. This is the first report on micropropagation of T. catappa, which can be applied for further genetic transformation assays and pharmaceutical purposes.

Efficient micropropagation and assessment of genetic fidelity of Boerhaavia diffusa L- High trade medicinal plant

“…In the present study, frequency of shoot induction, MNS/E and MLS/E were decreased with increasing concentrations of BAP. Similar to our observation, the favorable response of nodal segments was also reported in a number of plants like Salvadora persica (Phulwaria et al 2011), Salvadora oleoides (Shekhawat et al 2012), Terminalia bellirica (Phulwaria et al 2012a) and Terminalia catappa (Phulwaria et al 2012b). Our observations were superior to earlier report of Sudarshana et al (2008) and Roy (2008) in terms of frequency of shoot induction from nodal explants of Boerhaavia diffusa.…”

“…Many investigators have reported genetic stability of several micropropagated plants viz. Sapindus trifoliatus L (Asthana et al 2011), Andrographis paniculata (Dandin and Murthy 2012), Rauvolfia tetraphylla L. (Faisal et al 2012), Terminalia catappa (Phulwaria et al 2012b).…”

A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

“…1 B). Repeated transfer of the original explants was suggested as an efficient technique for rejuvenation and reinvigoration of in vitro cultures ( Sanchez et al 1997 ), which was further supported by subsequent reports on different plants ( Abraham et al 2010 ; Ram and Shekhawat 2011 ; Phulwaria et al . 2012 b ).…”

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

“…Ex vitro rooted plants were successfully acclimatized with 96.6±2.8 % of transplantation survival rate. The ex vitro rooting is not only helpful in reducing resources, time, and labor costs but also simplifies the protocol by eliminating the rooting step under sterile conditions (Preece and Shutter 1991;Dinkel-Meier et al 1993;Pospisilova et al 1999;Yan et al 2010;Phulwaria et al 2012b). Ex vitro rooting of shoots is advantageous in terms of better root system, time reduction, and ease of acclimatization (Yan et al 2010;Benmahioul et al 2012).…”