Micropropagation of Narcissus (Daffodils)

Harvey, B. M. R.; Selby, Christopher P.

doi:10.1007/978-3-662-03354-8_17

Cited by 4 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several wild species of this genus are also endemic to restricted territories and threatened in the Iberian Peninsula, according to the International Union for the Conservation of Nature (https://www.iucnredlist.org/) and national (Bañares et al, 2010) or regional (DOCM, 1998) checklists. Although micropropagation of Narcissus cultivars has been quite extensively studied (Hussey, 1982;Squires et al, 1991;Chow et al, 1992;Harvey and Selby, 1997;Langen-Gerrits and de Klerk, 1999;Sage et al, 1999;Sochacki and Orlikowska, 2005;de Klerk, 2012;Bach, 2016, 2017), information on micropropagation of wild species is rather limited. Successful in vitro production has been achieved for N. bulbocodium (Santos et al, 1998), N. triandrus (Santos and Salema, 2000), N. asturiensis (Santos et al, 2002) and N. tazetta (Rahimi Khonakdari et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Acta Biologica Cracoviensia s. Botanica

Vicedo¹,

Pavlov²,

Ríos³

et al. 2020

View full text Add to dashboard Cite

The genus Narcissus has several endemic, rare and/or threatened species in the Iberian Peninsula and North Africa. In vitro propagation is a useful tool for threatened plants conservation used in ex situ strategies. Thus, the aim of this work was to study the propagation in vitro of bulb scale explants of five endemic, rare and/or endangered Narcissus species from the Iberian Peninsula, treated with different PGR combinations. Initiation was achieved in half-strength Murashige and Skoog (MS) basal salts and vitamins, 10 g/L sucrose, 500 mg/L casein hydrolysate, 2 mg/L adenine, 10 mg/L glutathione and 5.5 g/L plant agar. In the multiplication phase, the highest bulblet proliferation was obtained in MS medium supplemented with 30 g/L sucrose and the combination of 10 μM 6-Benzylaminopurine (BAP) + 5 μM α-Naphthaleneacetic acid (NAA) in N. alcaracensis, N. eugeniae and N. hedraeanthus; 20 μM BAP + 5 μM NAA in N. jonquilla and N. yepesii. The highest rooting was obtained with 5 μM NAA + 1 μM Indole-3-butyric acid (IBA) for all species (>75%) and more than 80% of the produced bulblets were successfully acclimatized.

show abstract