Mass propagation of Cyrtanthus clavatus and Cyrtanthus spiralis using liquid medium culture

Morán, G.P; Colque, R.; Viladomat, Françesc; Bastida, Jaume; Codina, Carles

doi:10.1016/s0304-4238(02)00142-5

Cited by 13 publications

(3 citation statements)

References 24 publications

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“…The highest number of shoots as well as the percentage of explants producing shoots in Cyrtanthus loddigesianus and Cyrtanthus speciosus was achieved with high doses of NAA and BA (5.37 and 8.8 μM, respectively; Angulo et al 2003). These results are consistent with those observed by others researching Cyrtanthus species (McAlister et al 1998;Morán et al 2003;Hong and Lee 2012), other members of the Amaryllidaceae (Slabbert et al 1993;Vishnevetsky et al 2003), and bulbous species from other families (Cheesman et al 2010). While a number of research findings are consistent with this observed trend on bulb explants, several others have yielded results in sharp contrast.…”

Section: Resultssupporting

confidence: 90%

“…The combination resulted in the highest shoot frequency for this species, although it was not significantly different from the 5.0 μM TDZ treatment. The synergistic effect of auxins with CKs on shoot multiplication has been reported in studies of other Cyrtanthus species (Angulo et al 2003;Morán et al 2003). However, none of these studies investigated the effects of different CKs on shoot morphogenesis of Cyrtanthus species.…”

Section: Resultsmentioning

confidence: 97%

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In vitro regeneration of Cyrtanthus species: ornamental plants with medicinal benefits

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Finnie

Staden

2014

In Vitro Cell.Dev.Biol.-Plant

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Cyrtanthus (Amaryllidaceae) is a genus of perennial geophytes, endemic to the southern African region. Destructive and indiscriminate harvesting of bulbs for medicinal and ornamental purposes has led to intensive decimation of the populations of most of these species in their natural habitats. The aim of this study was to develop in vitro regeneration systems for Cyrtanthus contractus, Cyrtanthus guthrieae, and Cyrtanthus obliquus using twin-scale explants from mature bulbs. Twin scales from the three species were cultured on solid Murashige and Skoog (MS) medium with different concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA) under 16/8-h light/dark conditions at 25±2°C. The best shoot induction responses were obtained on MS medium containing 4.4 μM BA+1.1 μM NAA (3.1 shoots/explant) for C. contractus and C. guthrieae and 6.7 μM BA+2.7 μM NAA for C. obliquus. When the best shoot induction medium for each species was investigated for the effect of cytokinins on shoot organogenesis, using different concentrations of BA, kinetin (Kin), meta-topolin (mT), zeatin (ZT), and thidiazuron (TDZ), the medium that produced the best shoot induction for C. guthrieae also produced the highest number of shoots per explant, whereas the highest numbers of shoots per explant were obtained with 10 μM TDZ for C. contractus and 10 μM BA for C. obliquus. Superior quality shoots in all three species were obtained from MS medium supplemented with Kin and mT. Regenerated shoots were rooted successfully on half-and full-strength MS medium without plant growth regulators, transferred to organic soil mix, and successfully acclimatized in the greenhouse. The micropropagation protocols described provide rapid and cost-effective methods for in vitro propagation for application to the conservation and domestication of Cyrtanthus species.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 97%

In vitro regeneration of Cyrtanthus species: ornamental plants with medicinal benefits

Ncube

Finnie

Staden

2014

In Vitro Cell.Dev.Biol.-Plant

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“…R.A. Dyer and Cyrtanthus spiralis Burch. ex Ker Gawl., the twin scales were cultured in a liquid medium to increase the bulb production rate under aseptic conditions (Morán et al, 2003). However, Angulo et al (2003) followed the protocols for the micropropagation of Cyrtanthus loddigesianus (Herb.)…”

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confidence: 99%

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

et al. 2014

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K. Paredes, C. Delaveau, P. Carrasco, C. Baeza, Freddy Mora, and M.E. Uribe. 2014. In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile. Cien. Inv. Agr. 41(2): 207-214. Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant, respectively. The results showed high contamination of the bulbs. There were no significant differences between the treatments with plant growth regulators. A multiplication rate of 1.3 -2.2 for bulbils was achieved, with an average of 28 bulbils per mother bulb. The natural regeneration rate is approximately 1 -4 bulbils per mother bulb once a year. T. modesta is a plant characterized by its small size. It measures 15-20 cm in height, with linear or slightly swollen leaves, which are dry or non present at the time of blooming. Flowers measure 15-20 mm in length and are pure white or white with purple veins near towards to the base, with a purple line along the back of the tepals. The fruits are capsules that contain black-shiny seeds that are flattened, rounded and with membranous edges. In its natural habitat in Chile, T. modesta blooms from January to May (Baeza et al., 2009).Studies related to vegetative propagation of T. modesta are unknown; however, it is known that geophytes from Amaryllidaceae have a low natural propagation rate that ranges from approximately zero to eight bulbs per mother bulb (Santos et al., 1998; Schiappacasse et al., 2002;Angulo et al., 2003). Conventional propagation techniques for ornamental purposes have been used for the recovery of other species of endangered bulbous plants. For example, Herbertia lahue (Mol.) Goldbl. is a species from which Morales et al. (2007) obtained 1.13 bulbils per bulb, which is within the expected range found for other Chilean geophyte species such as Calydorea xiphioides Poepp. and Leucocoryne coquimbensis F. Phil (Kim et al., 1998), which produced 1.5 and 1.1 to 1.6 bulbils per mother bulb, respectively (Morales et al., 2007).In studies performed keep carried out by Schiappacasse et al. (2002), 18 species of Chilean geophytes with ornamental potential were propagated. In these studies, a maximum of 8.6 bulbils per initial bulb were obtained by using bulb division as the method for vegetative propagation. When performing the twin-scaling separation technique with Rhodophiala bagnoldii (Herb.) Traub, the number increased to 30 bulbils per mother bulb, demonstrating the potential of these techniques.To compare the karyotype of T. modesta with a species of the same family, Baeza et al. (2009) conduct...

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In vitro propagation via organogenesis and embryogenesis of Cyrtanthus mackenii: a valuable threatened medicinal plant

Kumari

Baskaran

Staden

2017

Plant Cell Tiss Organ Cult

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Mass propagation of Cyrtanthus clavatus and Cyrtanthus spiralis using liquid medium culture

Cited by 13 publications

References 24 publications

In vitro regeneration of Cyrtanthus species: ornamental plants with medicinal benefits

In vitro regeneration of Cyrtanthus species: ornamental plants with medicinal benefits

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

In vitro propagation via organogenesis and embryogenesis of Cyrtanthus mackenii: a valuable threatened medicinal plant

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