Micropropagation of Philodendron selloum: Influence of copper sulfate on endophytic bacterial contamination, antioxidant enzyme activity, electrolyte leakage, and plant survival

Seliem, Mayada Kadry; El-Mahrouk, Mohammed E.; El-Banna, Antar; Hafez, Y. M.; Dewir, Yaser Hassan

doi:10.1016/j.sajb.2021.01.024

Cited by 16 publications

(28 citation statements)

References 70 publications

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“…After 4 days, WT and transgenic lines all showed obvious wilting, but the growth status of the transgenic lines was slightly better than that of WT ( Figure 6 a). Electrolyte leakage and malondialdehyde (MDA) are reference indicators of the degree of plant damage [ 48 , 49 ]. Electrolyte leakage of the three transgenic lines was 65.24%, 68.76%, and 69.35%, respectively, all significantly lower than that of WT (85.27%) ( Figure 6 b).…”

Section: Resultsmentioning

confidence: 99%

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CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

Wang

Cao

et al. 2021

Genes

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Zinc-finger proteins are important transcription factors in plants, responding to adversity and regulating the growth and development of plants. However, the roles of the BBX gene family of zinc-finger proteins in wintersweet (Chimonanthus praecox) have yet to be elucidated. In this study, a group IV subfamily BBX gene, CpBBX19, was identified and isolated from wintersweet. Quantitative real-time PCR (qRT-PCR) analyses revealed that CpBBX19 was expressed in all tissues and that expression was highest in cotyledons and inner petals. CpBBX19 was also expressed in all flower development stages, with the highest expression detected in early initiating bloom, followed by late initiating bloom and bloom. In addition, the expression of CpBBX19 was induced by different abiotic stress (cold, heat, NaCl, and drought) and hormone (ABA and MeJA) treatments. Heterologous expression of CpBBX19 in Arabidopsis thaliana (Arabidopsis) enhanced the tolerance of this plant to salt and drought stress as electrolyte leakage and malondialdehyde (MDA) concentrations in transgenic Arabidopsis after stress treatments were significantly lower than those in wild-type (WT) plants. In conclusion, this research demonstrated that CpBBX19 plays a role in the abiotic stress tolerance of wintersweet. These findings lay a foundation for future studies on the BBX gene family of wintersweet and enrich understanding of the molecular mechanism of stress resistance in wintersweet.

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Section: Resultsmentioning

confidence: 99%

“…MDA content and electrolyte leakage of transgenic Arabidopsis were detected as indicators of stress tolerance. MDA content was determined as described by Heath et al [ 48 ], while the method of electrolyte leakage was stated by Seliem et al [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

Wang

Cao

et al. 2021

Genes

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“…Banana plants are commercially propagated through the tissue culture technique [ 2 ], which can provide mass propagation, rejuvenation of older varieties, disease elimination, conservation of genetic resources, and the management of abiotic and biotic stresses [ 3 , 4 ]. Microbial contamination is one of the most important obstacles, as it prevents the successful micropropagation of in vitro plants [ 5 ]. Therefore, several methods have been used to obtain aseptic cultures of explants, such as ultraviolet, autoclaving of media [ 6 ] or the use of chemical antiseptic agents like sodium hypochlorite and mercuric chloride, whereas, antibiotics and copper sulphate can be used for eliminating the endogenous contamination [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Microbial contamination is one of the most important obstacles, as it prevents the successful micropropagation of in vitro plants [ 5 ]. Therefore, several methods have been used to obtain aseptic cultures of explants, such as ultraviolet, autoclaving of media [ 6 ] or the use of chemical antiseptic agents like sodium hypochlorite and mercuric chloride, whereas, antibiotics and copper sulphate can be used for eliminating the endogenous contamination [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…As an essential micronutrient for plant growth, copper (Cu) is needed for several physiological functions, including photosynthesis, respiration, oxidative stress response, electron transport, plant hormone signaling, and cell wall biosynthesis and its lignification [ 7 ]. As a cofactor in many enzymes, Cu has a crucial role in many plant enzymes such as amino oxidase, laccase, superoxide dismutase (SOD), and polyphenol oxidase [ 5 ]. Copper and its compounds are important ingredients in several antibacterial and antifungal compounds, which have been applied against a wide range of pathogens including viruses, fungi and bacteria [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Elyazid

Salama

Zanaty

et al. 2021

Plants

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Developing a successful protocol for banana in vitro culture is a guarantee for the mass propagation of pathogen-free, high-quality, true-to-type planting materials with low production costs. The current work aimed to investigate the influence of increasing copper levels in an MS medium on endophytic bacterial contamination; shoot multiplication; rooting and the acclimatization of in vitro cultured banana; minerals and chlorophyll content; antioxidant enzymes activity; electrolyte leakage; and the genetic stability of banana regenerants. Four different concentrations of copper sulphate (0.025 as a control, and 30, 60, and 120 mg L−1) were examined. The growth of the endophytic bacteria was inhibited at 60 mg L−1 of copper sulphate which recorded zero contamination, without a significant difference at 120 mg L−1. However, 0.025 mg L−1 of copper sulphate was optimal for the maximum shoot number and shoot length (10 shoots and 6 cm, respectively) without significant differences at 30 mg L−1. The root length of banana plantlets was significantly enhanced at 30 mg L−1 of copper sulphate but without significant differences to the control, regarding the number of roots (9.92 cm and 3.80 roots, respectively). In vitro plants were acclimatized successfully at 30 mg L−1 of copper sulphate with 100% survival. The uptake of minerals, antioxidant enzyme activity and electrolyte leakage was improved because of the copper sulphate, but the chlorophyll level decreased. RAPD profiling showed polymorphism in only one plant treated with 60 mg L−1 of copper sulphate, with an average of 1.8%. The genome template stability percentage was almost 100% for all treated plants.

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Application of antimicrobial properties of copper

Yu,

Huang,

Ren

et al. 2024

Applied Organom Chemis

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The antimicrobial properties of copper ions can reduce the overgrowth of microorganisms to a certain extent. That reduces the amount of antibiotics used, to avoid superbugs and potential harm. The authors reviewed the antimicrobial mechanisms of copper and copper‐containing nanoparticles (NPs), focusing on the factors influencing their antimicrobial properties. They concluded that the antimicrobial properties were better in environments conducive to the precipitation of copper ions and exposure to microorganisms. Comparing copper with other metal ions leads to the question of why copper is such an important antimicrobial metal ion. In recent years, with the development of science and technology and the understanding of the microscopic world, scientists have skillfully applied the antimicrobial properties of copper in various settings, from direct contact with food to breeding and hygienic environments, and have confirmed the safety of the application. However, because of the toxic effect of NPs, the future goal is to synthesize a copper‐containing NP with good biocompatibility and antibacterial efficiency to benefit human society.

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Micropropagation of Philodendron selloum: Influence of copper sulfate on endophytic bacterial contamination, antioxidant enzyme activity, electrolyte leakage, and plant survival

Cited by 16 publications

References 70 publications

CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

CpBBX19, a B-Box Transcription Factor Gene of Chimonanthus praecox, Improves Salt and Drought Tolerance in Arabidopsis

In Vitro Propagation and Acclimatization of Banana Plants: Antioxidant Enzymes, Chemical Assessments and Genetic Stability of Regenerates as a Response to Copper Sulphate

Application of antimicrobial properties of copper

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