Micropropagation of Piper crassinervium: an improved protocol for faster growth and augmented production of phenolic compounds

Ríos-Ríos, Anyela Marcela; Silva, José Victor Siqueira da; Fernandes, João Victor Marçal; Batista, Diego Silva; Silva, Tatiane Dulcineia; Chagas, Kristhiano; Pinheiro, Marcos Vinícius Marques; Faria, Daniele Vidal; Otoni, Wagner Campos; Fernandes, Sérgio Antônio

doi:10.1007/s11240-019-01585-8

Cited by 5 publications

(4 citation statements)

References 81 publications

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“…These changes are due to improved gas exchange efficiency which stimulates photosynthesis while positively influencing plant performance both when in vitro and in the ex vitro acclimatization (Kozai and Kubota 2001;Xiao et al 2011). The use of gas-permeable membranes during the in vitro stage not only leads to more vigorous plants with improved biomass accumulation, higher growth and photosynthetic rates, and larger, better-developed leaves and roots, but also stimulates nutrient absorption and promotes secondary metabolism (Ribeiro et al 2009;Arigita et al 2010;Kozai 2010;Badr et al 2011;Iarema et al 2012;Saldanha et al 2013Saldanha et al , 2014Ríos-Ríos et al 2019). Some of these benefits were confirmed here in plants under 1000 µmol mol −1 CO 2 , gas-permeable membranes, and sucrose in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

“…To achieve this, gaspermeable membranes (Kozai 2010;Saldanha et al 2012) have been shown to enable greater gas exchange, decrease ethylene accumulation into the flask, and facilitate plant acclimatization (Xiao et al 2011). Moreover, they have a positive effect on culture establishment, improving shoot quantity and quality, while reducing the occurrence of chlorosis and leaf senescence (Rodrigues et al 2012;Ríos-Ríos et al 2019). Finally, they can increase plant survival during acclimatization by hardening in vitro-grown plants (Batista et al 2017a, b).…”

Section: Introductionmentioning

confidence: 99%

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CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

et al. 2021

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Conventional in vitro propagation may affect normal morphophysiological characteristics and survival during the acclimatization of commercial plants, such as Etlingera Elatior (Jack) R.M. Smith. CO 2 enrichment, gas-permeable membranes, and reduced carbohydrate concentration in culture media can be used to mitigate these effects. Here, we aimed to determine the impact of photomixotrophic and photoautotrophic growth on morphophysiological responses of E. elatior and its survival during ex vitro acclimatization. In vitro-established plants were placed in flasks containing semisolid MS medium with 0.54 µM α-naphthalene acetic acid and sucrose (0 or 15 g L −1 ). The flasks were sealed with lids with or without orifices covered by gas-permeable membranes and kept for 45 days under forced-air ventilation (360 or 1000 µmol −1 CO 2 ). Growth variables, anatomical and physiological characteristics, and survival rate were evaluated. Overall, 1000 µmol mol −1 CO 2 , gas-permeable membranes, and 15 g L −1 sucrose resulted in plants with higher fresh and dry mass, higher level of photosynthetic pigments, as well as more developed secondary ribs in the leaves, prominent midribs, highly organized and differentiated chlorenchyma cells (palisade and spongy parenchyma), more developed vascular bundles, and intercellular gaps were reduced, compared to conventionally in vitro-propagated plants. Moreover, these conditions promote morphophysiological responses and favored survival of E. elatior plants (75%) when transferred to ex vitro conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

et al. 2021

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“…BA and other cytokinins were reported to increase phenolic content of in vitro-grown tissues [30,31]. 2-iPA increased the total flavonoid content in the culture of Piper crassinervium [32]. Experiments performed by Sidkey showed that cytokinins markedly enhanced the accumulation of alkaloids in C. roseus cultures by an increased expression of geranil 10-hydroxlase gene [33].…”

Section: Discussionmentioning

confidence: 99%

Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

Sarkar

Banerjee

2021

Planta Medica International Open

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Steroid alkaloid solasodine is a nitrogen analogue of diosgenin and has great importance in the production of steroidal medicines. Solanum erianthum D. Don (Solanaceae) is a good source of solasodine. The aim of this study was to evaluate the effect of different cytokinins on the production of secondary metabolites, especially solasodine in the in vitro culture of S. erianthum. For solasodine estimation, field-grown plant parts and in vitro tissues were extracted thrice and subjected to high-performance liquid Chromatography. Quantitative analysis of different secondary metabolites showed that the amount was higher in the in vitro regenerated plantlets compared to callus and field-grown plants. The present study critically evaluates the effect of the type of cytokinin used in the culture medium on solasodine accumulation in regenerated plants. The highest solasodine content (46.78±3.23 mg g-1) was recorded in leaf extracts of the in vitro grown plantlets in the presence of 6-γ,γ-dimethylallylamino purine in the culture medium and the content was 3.8-fold higher compared to the mother plant.

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“…The complexity of phenolic compounds hinders their artificial production, and biotechnological manipulation of plant tissue culture conditions is a safe alternative to increase their production (Dias et al, 2016). Gas exchange and the reduction of sugar in the culture medium force the plantlets to develop a photomixotrophic metabolism, which stimulates the production of phenolic compounds (Ríos-Ríos et al, 2019). This increase in the production of phenolic compounds is supported by the physiological improvements resulting from photomixotrophic propagation, such as reduced stress signaling caused by excess sugar (Badr et al, 2011;Horacio & Martinez-Noel, 2013) and reduced inhibition of photosynthesis (Couto et al, 2014).…”

Section: Analysis Of Total Phenolic Compounds (Tpc)mentioning

confidence: 99%

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

Coelho

Leite²,

Santos³

et al. 2022

RSD

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The objective of this study was to evaluate the effect of the use of different natural ventilation systems on the growth and accumulation of total phenolic compounds in Aloysia gratissima. Nodal and apical segments with 1 pair of leaves were inoculated in flasks containing ½ MS culture medium without sucrose and supplemented with 0.1 mg L-1 indole-butyric acid. The cultivation systems included a conventional system (NMS) and alternative membrane systems with 1 (AMS1), 2 (AMS2) and 4 (AMS4) low-cost membranes. After 30 days, the in vitro growth, leaf area, dry weight and accumulation of phenolic compounds were evaluated. Plantlets from apical segments showed superior results in all variables analyzed. The use of the NMS compromised plantlet growth. However, improved results were obtained with the use of porous membranes, with the best growth observed in AMS4. The leaf area of plantlets originating from apical segments was 3.03 times greater than that of plantlets from nodal segments. Plantlets in the NMS had the lowest values of leaf area, root length, number of leaves and dry weight. However, the use of membranes allowed higher growth. The AMS4 treatment increased the leaf dry weight accumulation by 6.13-fold compared to that obtained with the NMS treatment. The accumulation of total phenolic compounds increased with the use of greater numbers of porous membranes. The use of apical segments and lids with 4 porous membranes is recommended for micropropagation of the species. The use of an alternative membrane system positively influences the accumulation of total phenolic compounds.

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Micropropagation of Piper crassinervium: an improved protocol for faster growth and augmented production of phenolic compounds

Cited by 5 publications

References 81 publications

CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

CO2 enrichment alters morphophysiology and improves growth and acclimatization in Etlingera Elatior (Jack) R.M. Smith micropropagated plants

Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

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