Micropropagation of Potato (Solanum tuberosum L.)

Dodds, John H.; Silva-Rodriguez, D.; Tovar, P.

doi:10.1007/978-3-662-07770-2_6

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…Alternatively, in more efficient and common systems the potato plant is first micropropagated in a low sucrose, hormone-free medium, and then induced to form tubers on the node explants by increasing sucrose content and supplying growth regulators to the medium, usually gibberellin synthesis inhibitors and cytokinins (Dodds et al, 1992). In these systems micropropagation is usually under long photoperiod (Ewing, 1995), while short photoperiod or continuous darkness are preferred for tuber induction (Dodds et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…In these systems micropropagation is usually under long photoperiod (Ewing, 1995), while short photoperiod or continuous darkness are preferred for tuber induction (Dodds et al, 1992). Although the systems are able to produce a high quantity of microtubers, their variations in size and in subsequent performance cause difficulties which vary depending, among others, on the cultivar and on the exact protocols (Jones, 1988;Leclerc et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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In vitro induction of potato tuberization by organic acids

1999

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SummaryPotato microtubers are usually induced in media containing growth regulators, typically cytokinins and growth retardants. However, since these substances may imbalance the physiology of the cultured explants and cause adverse carry-over effects on the subsequent performance of the microtubers, the potential acid-induction of in vitro-mass tuberization was investigated as an alternative. For this purpose, a range of explant types (stolons, single-node or apex-containing multinodal sections), organic acids (acetic, propionic, ascorbic, acetylsalicylic or salicylic acid), and photoperiods (continuous dark, 8 or 16 h photoperiod) were established. Gellified medium or raft membranes on liquid medium were also tested.Although variations due to explant type, photoperiod, organic acid supplemented, and medium type were found, all organic acids tested under the different experiments caused tuberization. Multiple tuber formation or bigger tubers did not develop on multinodal explants. In terms of tuberization rate and mean tuber weight, gellified medium performed better than liquid medium with rafts. The recently established role of the salicylic and acetylsalicylic acids on tuberization is corroborated by our system. The results with the acetic, propionic and ascorbic acids show that in vitro hormone-free tuberization can be easily and rapidly achieved.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro induction of potato tuberization by organic acids

1999

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“…To ensure maximum rate of microtuberization dur ing experimental research, media are routinely sup plemented with a cytokinin and an inhibitor of GA biosynthesis in addition to a high sucrose level (Estrada et al, 1986;Dodds et al, 1992;Kumar et al, 1995). However, as plant growth regulators in tissue culture media tend to affect subsequent microtuber dormancy and sprouting quality after outplanting to the soil, commercial micropropagation laboratories prefer to utilize media with a high sucrose content and devoid of such growth substances.…”

Section: The Roles Of Carbohydrates Supplementing Tissue Culture Mediamentioning

confidence: 99%

“…However, it is presented here specifically as a means to learn about storage organ regeneration in culture. The methodologies of microtuber regeneration in culture have been found to be valid for a range of Solarium species (Kumar et al, 1995), and a wide spectrum of Solarium tuberosum L. cultivars (Estrada, Tovar, and Dodds, 1986;Dodds, Silva-Rodriguez, and Tovar, 1992). The procedures are simple, reliable, and inexpen sive.…”

Section: Introductionmentioning

confidence: 98%

An exercise in the formation of potato microtubers in tissue culture

Steinitz

1997

Journal of Biological Education

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“…Several protocols to induce in vitro tuberization have already been developed, such as that of Wang and Hu (1982), which is adopted by the International Potato Center (Centro Internacional de la Papa -CIP) (TOVAR et al, 1985;SILVA-RODRIGUEZ;TOVAR, 1992). This protocol consists of adding 6benzylaminopurine, chlorocholine chloride and sucrose to the liquid culture medium.…”

Section: Tuberizationmentioning

confidence: 99%

Application of tissue culture techniques in potato

Morais¹,

Asmar²,

Silva³

et al. 2018

Biosci. J.

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Potato is the world's most important non-cereal food crop, and therefore, it is considered one of the major food sources for humankind. Its conventional propagation is asexual, by using the tuber, which allows the accumulation and dissemination of pathogens to new cultivation areas. This fact not only impairs the yield of this solanaceous plant, but also threatens the maintenance of genotypes for commercial or breeding purposes. Due to the impossibility of using botanical seed, conservation and exchange of germplasm of this species by means of conventional methods are not feasible. In all potato-producing regions, the demand for high-quality tubers has been paramount to ensure crops production. Thus, biotechnological techniques based on tissue culture are very important. Plant tissue culture offers alternative methods of propagation by in vitro techniques that provide production and multiplication of material with high sanity. Thus, this literature review summarizes the history and current situation of tissue culture techniques applied to potato crop. Besides clonal multiplication, this biotechnological tool makes available initial indexed material to breeding programs and certified seed potato, and facilitates the exchange and conservation of germplasm. For all these reasons, the use of these techniques in potato production chain directly benefits producers by providing high-quality propagules.

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Micropropagation of Potato (Solanum tuberosum L.)

Cited by 14 publications

References 12 publications

In vitro induction of potato tuberization by organic acids

In vitro induction of potato tuberization by organic acids

An exercise in the formation of potato microtubers in tissue culture

Application of tissue culture techniques in potato

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