Micropropagation of Stemona Curtisii Hook F., a Thai Medicinal Plant

Montri, N.; Wawrosch, Christoph; Kopp, B.

doi:10.17660/actahortic.2006.725.43

Cited by 2 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, incubation in MS medium without PGRs was needed for shoot elongation, consistent with our previous findings in Curcuma longa (Prathanturarug et al 2003;Prathanturarug et al 2005), Gagnepainia godefroyi, and G. thoreliana (Prathanturarug et al 2007). TDZ has also been used for shoot induction in Stemona (Montri et al 2006;Singlaw et al 2008). Singlaw et al (2008) reported a shoot induction rate of 3.1 shoots/explant in S. tuberosa, using 4.54 μM TDZ.…”

Section: Resultssupporting

confidence: 88%

“…Singlaw et al (2008) reported a shoot induction rate of 3.1 shoots/explant in S. tuberosa, using 4.54 μM TDZ. In S. curtisii, TDZ was shown to induce a number of shoots with stunting and thickening of stems (Montri et al 2006). Rungruchkanont and Pongrat (2008) reported a shoot induction in S. collinsae using 22.22 μM BA in nodal, but not internodal, explants.…”

Section: Resultsmentioning

confidence: 99%

“…All previously published papers used meristematic tissues as initial explants, including terminal bud explants in Stemona sp. (Chotikadachanarong et al 2005), nodal explants in S. collinsae (Rungruchkanont and Pongrat 2008), shoot tip explants in S. tuberosa (Singlaw et al 2008), and shoot explants in S. curtisii (Montri et al 2006). We used TDZ, which has been shown to be one of the most potent plant growth regulators for woody species (Huetteman and Preece 1993).…”

Section: Resultsmentioning

confidence: 99%

“…Root induction appeared to be difficult to achieve in S. hutanguriana compared to other Stemona spp. (Montri et al 2006;Rungruchkanont and Pongrat 2008). There-fore, a two-step protocol which increased cuture period and time taken to produce plantlets was needed for S. hutanguriana.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, sexual propagation by seeds is very poor (Rungrojsakul 2001). Therefore, a number of in vitro propagation protocols based on organogenesis have been established for Stemona spp., including those for S. collinsae (Rungruchkanont and Pongrat 2008), S. curtisii (Montri et al 2006), S. tuberosa (Singlaw et al 2008), and Stemona sp. (Chotikadachanarong et al 2005).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

Prathanturarug

Pheakkoet

Jenjittikul

et al. 2012

Physiol Mol Biol Plants

View full text Add to dashboard Cite

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.

show abstract