Micropropagation of the Endangered Shrub Pondberry (Lindera melissifolia [Walt.] Blume)

Abstract

<jats:p>A micropropagation protocol using shoot cultures is described for <jats:italic>Lindera melissifolia</jats:italic>, a federally listed endangered shrub endemic to the southeastern United States. Stock plants were harvested from native <jats:italic>L</jats:italic>. <jats:italic>melissifolia</jats:italic> populations growing in the lower Mississippi Alluvial Valley. In vitro proliferation was on woody plant medium supplemented with 1 μ<jats:sc>m</jats:sc> zeatin. After 6 weeks, zeatin level was increased to 5 μ<jats:sc>m</jats:sc>. Treatment of micropropagated shoots with a liquid auxin (2 indole-3-butyric acid : 1 1-naphthalenacetic acid) resulted in a low mean rooting percentage (≤44%) compared with rooting in the absence of auxins and on a pure peat medium ex vitro, which increased rooting to ≥80%. Time to rooting was 8 weeks. Plants were acclimatized for 2 weeks, then potted in a 2 peat : 1 perlite medium supplemented with superphosphate, 10N–10P–10K, and Milorganite. Micropropagated <jats:italic>L</jats:italic>. <jats:italic>melissifolia</jats:italic> stecklings have been successfully outplanted in both controlled and field studies at the Center for Bottomland Hardwoods Research (Stoneville, Miss.).</jats:p>

Micropropagation of the endangered Chilean tree, Gomortega keule

“…Zeatin merely stimulated callus production in G. keule (Table 1). Hawkins et al (2007) used zeatin to micropropagate Lindera melissifolia, another endangered woody plant of the Lauraceae family, but shoot proliferation was accompanied by callus production.…”

Modeling the relationship between extractable chlorophyll and SPAD-502 readings for endangered plant species research

“…Source plants used to develop the regression originated from micropropagation of Lindera melissifolia stockplants (20 genotypes) collected from selected bottomland forests in the Lower Mississippi Alluvial Valley (Hawkins et al 2007). Micropropagules were grown for approximately six months in a climate controlled greenhouse with a 14 h light (X300 mmol m À2 s À1 ):10 h dark photoperiod and mean temperature of 7876 1C.…”

Micropropagation of Spigelia marilandica L.

“…The mist-plus-dome protocol provided an environment of high humidity without saturating the rooting medium while maintaining microcutting quality as nutrients were retained rather than washed away due to the misting action (Wainwright and Scrace 1989). Others having success rooting microcuttings ex vitro have used flats covered with domes (Hawkins et al 2007) and flats enclosed in clear plastic bags (Kataoka and Inoue 1992). Rooting rates of S. marilandica microcuttings ranged from 100% (cold storage study), 97 to 100% (Table 3), and 88 to 97% (microcutting size study) after 2, 4, or 6 wk under mist in flats covered with propagation domes, respectively.…”