Micropropagation of the therapeutic-honey plants

Darby, Ian D.; Bai, Shahla Hosseini; Wallace, Helen M.; Trueman, Stephen J.

doi:10.1071/bt21047

Cited by 7 publications

(4 citation statements)

References 46 publications

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“…Indeed, the same authors mentioned a significant decrease in bud break rate between the second and third distal nodes probably due to the negative morphogenetic gradient. However, Darby et al [16] found that the original position of the explant in in vitro culture did not influence shoot regeneration in Leptospermum polygalifolium and Leptospermum scoparium. The addition of 0.1mg/L NAA to medium containing 2mg/L BAP was able to stimulate bud break of seeded internodes [17].…”

Section: Discussionmentioning

confidence: 99%

Topophysis and Growth Regulators Effects on Buds and Zygotic Embryos Regeneration of African Bush Mango Tree Cultured In vitro

Agbidinoukoun

Noumon

Quenum

et al. 2022

ARRB

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Background: African bush mango tree is an important fruit plant with high nutritional, medicinal, and commercial values. However, its seedling system remained a deep understanding. This study aimed to evaluate the effect of topophysis and growth regulators on the reactivity of different types of buds and zygotic embryos of wild mango. Methodology: Ripe fruits from two local varieties (Wossro and Sissro) were pulped. The nuts were extracted and dried for one week under greenhouse. Zygotic embryos were excised from nuts and disinfected with the bleach solution (NaClO 10% with 8% active chloride) for 10 min following by three successive rinsing using sterilized water. The second set of nuts was cultivated for under greenhouse in the polybags containing the sand. One month later, buds from different positions (apical, axillary and cotyledonary) were excised and disinfected with NaClO 10% for 10 min follow by the immersion in a mercuric chloride 0.1% added with two drops of Tween 20 especially for axillary and cotyledonary buds for 5 min while 0.01% of mercuric chloride was used for apical buds. The explants were cultured on ¼MS and ½WPM media supplemented with BAP, KIN at different concentrations (0.2 mg/L and 3.5 mg/L respectively) and NAA (0.05 mg/L). Results: The best sprouting rate was obtained with the variety Wossro which showed a high bud break rate (26.47%) against (9.88%) for the variety Sissro. The topophysis significantly influenced the response of the buds in tissue culture. 24.48% of axillary buds were sprouted on ¼ MS medium + 3.5 mg/L BAP+ 0.05mg/L NAA. The in vitro germination of embryos was significantly (p≤ 0.05) influenced by the genotype of the variety. 50.76% of buds were germinated for Wossro while 18.32% were germinated for Sissro. ¼ MS + 0.2 mg/L KIN has significant influenced the plants growth and development. Implication: The findings will help to improve the regeneration rate and plantlets production of African bush mango tree.

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Section: Discussionmentioning

confidence: 99%

Topophysis and Growth Regulators Effects on Buds and Zygotic Embryos Regeneration of African Bush Mango Tree Cultured In vitro

Agbidinoukoun

Noumon

Quenum

et al. 2022

ARRB

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“…In some clones, this dose resulted in extremely high shoot numbers; explants frequently produced extensive callus and multiple short shoots. PGRs play an important role in cytodifferentiation and morphogenesis, regulating the complex processes of cell proliferation, xylogenetic differentiation, and root-shoot differentiation [41].…”

Section: Multiplication Of L Suaveolensmentioning

confidence: 99%

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitations

Putri¹,

Kartikawati²,

Nirsatmanto³

et al. 2022

Preprint

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Peatlands in Indonesia are one of the world's largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a plant found in Papua's endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland re-habilitation. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. The Murashige and Skoog (MS) medium was sup-plemented with single and double kinds of PGRs (BAP and NAA). The concentration used in this study is based on the best findings of earlier preliminary research on single PGR treatment (0.5 ml/l BAP and 0.1 ml/l NAA) for the initiation stage. After 3 months of in vitro axillary shoot initiation, elongated shoots from plant-lets were separated from the clumps and multiplied. Until 6 months incubation, the potential multiplication of axillary shoots was determined by counting the number of nodule multiplication coefficients (NMC), shoot length, root length, and number of leaves (6 successive subculture). The highest average per plantlet of NMC (8.4 0.13) was observed in MS medium with a combination of 0.5 ml/l BAP and 0.1 ml/l NAA (double PGRs), with the longest shoots (5.91 0.17 cm), the longest root length (8.83 0.69 cm), and the most leaves (32 0.69). They were cultured in a pH 5 environment with a light photoperiod (40 mol/m2/s intensity) for 16 hours. When BAP and NAA were used together after acclimatization, seedling survival was highest. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale.

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Section: Multiplication Of L Suaveolensmentioning

confidence: 99%

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitation

et al. 2022

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Peatlands in Indonesia are one of the world’s largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a relatively unexplored plant found in Papua’s endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland rehabilitation. Seed is one of materials for the reproduction of L. suaveolens. However, the difficulty in seed collection and the limitation in seed production has become a current problem for its cultivation. Seed multiplication by using an in vitro method would be one of the mechanisms to overcome the problem. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. After 3 months of the culture initiation stage, the elongated axillary shoots were separated from the clumps and further multiplied using Murashige and Skoog (MS) media supplemented with (1) BAP (0.5 mL/L) as single PGR, (2) NAA (0.1 mL/L) as a single PGR, and (3) a combination of two types of PGR BAP (0.5 mL/L) and NAA (0.1 mL/L). Up to an incubation period of 6 months, the efficiency of leaf axillary shoot propagation was determined by counting the number of nodule multiplication coefficient (NMC), shoot length, root length, and number of leaves (six consecutive subcultures). The higher the NMC, the higher the plantlets obtained, increasing shoot regeneration from nodules physiologically increasing evapotranspiration in vitro. The highest of NMC (8.4) was observed in MS medium with a combination of 0.5 mL/L BAP and 0.1 mL/L NAA (double PGRs), with the longest shoots (5.91 cm), the longest root length (8.83 cm), and the most leaves (32). When a combination of BAP and NAA were used simultaneously, the plantlets during acclimatization were the highest survived. It was concluded that MS in combination with 0.5 mL/L BAP and 0.1 mL/L NAA is the most appropriate protocol for the success of in vitro multiplication of L. suaveolens. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale. The authors acknowledge the limitations of the experimental work and recommend further work to increase the sample size and complete the field-testing phase to help verify the initial findings presented in this paper.

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Micropropagation of the therapeutic-honey plants

Cited by 7 publications

References 46 publications

Topophysis and Growth Regulators Effects on Buds and Zygotic Embryos Regeneration of African Bush Mango Tree Cultured In vitro

Topophysis and Growth Regulators Effects on Buds and Zygotic Embryos Regeneration of African Bush Mango Tree Cultured In vitro

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitations

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitation

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