Micropropagation of Three Citrus Rootstocks by Tissue Culture

Abstract

The experiments were conducted in the Laboratory of Tissue culture/Department of Forestry/College of Agriculture and Forestry/the University of Mosul. To propagate three citrus rootstocks (Citrus aurantium L., Citrumelo swingle, and Cariso 35 (C35))In vitro. During the Initiation stage, the single nodes were planted on a solid MS medium supplemented with five concentrations of BA (0, 1, 2, 3, and 4 mg L-1). After four weeks, the best significant percentage of response for Cariso35 and Citrumelo swingle, while the highest value of BA effect was at 2 mg BA L-1(96.67%). The No. of shoots there are nonsignificant differences, While the best No. of the shoot (1.41) obtained at 2 mg BA L-1, The highest significant shoot length (2.14cm) was obtained at Cariso35 compared with other rootstocks, so that, 1mg BA L-1 was achieved (2.01 cm). At the multiplication stage, Citrumelo swingle achieved the highest significant value (2.68shoots), whereas adding 4 mg BA L-1 gave the best significant No. shoots (3.10). as soon as, the shoot length (1.03cm) was obtained for Cariso35, while control treatment achieved a significant increase in shoot length (1.30cm) which differ significantly from other treatments. The highest significant number of leaves was (11.50 leaves) for Citrumelo swingle, while BA at 2 mgL-1 was dominated value achieved at 11.00 leaves. In the rooting stages, the best rooting percentage was 60% for Cariso 35 cultured on MT solid medium, but a non-significant difference between all IBA concentrations. While the significant No. of roots was 3.28 obtained from Citrumelo swingle, and the concentration 1.5 and 2 mg IBA L-1 achieved the highest significant values (2.90 and 2.24 root) respectively. Citrumelo swingle and the 1.5 mg IBA L-1 gave the shortest significant duration to formed roots which were 21.56 and 18.60 days respectively.

Comprehensive study on in vitro propagation of some imported peach rootstocks: in vitro explant surface sterilization and bud proliferation

“…These results are in agreement with those observed for the peach tree 35 and plum tree 29,32 . The superiority in the length of the shoots in the media that contained low concentrations of BAP (0.00 mg/L), compared to the other treatments could be attributed to the decrease in the number of shoots in these treatments, and thus the opportunity for them to obtain the nutrient from the medium increased compared to the treatments that contained high concentrations (3 and 5 mg/L) of BAP, which formed more shoots [56][57][58] .…”

Comprehensive Study on In vitro Propagation of Some Imported Peach Rootstocks a. In vitro Explant Surface Sterilization and Bud Proliferation

“…These results are in agreement with those observed for the peach tree [33] and plum tree ( [27]; [30]. The superiority in the length of the shoots in the media that contained low concentrations of BAP (0.00 mg/L), compared to the other treatments could be attributed to the decrease in the number of shoots in these treatments, and thus the opportunity for them to obtain the nutrient from the medium increased compared to the treatments that contained high concentrations (3 and 5 mg/L) of BAP, which formed more shoots ( [54]; [55]; [56]).…”