Micropropagation of Three Varieties of Caralluma adscendens via Nodal Explants

Aruna, Varimadugu; Chadipiralla, Kiranmai; Karuppusamy, S.; Pullaiah, T.

doi:10.1007/bf03263309

Cited by 24 publications

(20 citation statements)

References 9 publications

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“…The reason for the reduced survival in higher concentrations of NAA treatments may be due to poor vascular connection of the root with the stem because on the intervention of callus. The positive response of rooting in the present study is similar to observations of other species of Caralluma such as Caralluma adscendens (Aruna et al, 2009), Caralluma sarkariae (Sreelatha et al, 2009), Caralluma bhupenderiana (Ugraiah et al, 2011) and Caralluma pauciflora (Kiranmai et al, 2015).…”

Section: In Vitro Rooting Of In Vitro Regenerated Shoots and Acclimatsupporting

confidence: 89%

“…There are no cultivation techniques for this endemic succulent medicinal plant till now. For conservation of medicinal Caralluma species, in vitro protocol are already developed for Caralluma edulis (Kaur et al, 1992), Caralluma adscendens (Aruna et al, 2009), Caralluma sarkariae (Sreelatha et al, 2009), Caralluma bhupenderiana (Ugraiah et al, 2011), Caralluma tuberculata (Rehman et al, 2014) and Caralluma pauciflora (Kiranmai et al, 2015). We earlier described micropropagation method using nodal explants of Caralluma lasiantha (Aruna et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.

2016

IJSR

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Section: In Vitro Rooting Of In Vitro Regenerated Shoots and Acclimatsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.

2016

IJSR

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“…More than 70% calli produced shoots when cultured on BA containing medium while it was little less (62%) in case of Kin containing medium. The same was also found effective for induction of multiple shoots from nodal segments of C. adscendens (Aruna et al 2009) and C. bhupenderiana sarkaria (Ugraiah et al 2011). However, Naima & Taoufik (2006) reported high concentration of Kin with NAA for organogenic response form hypocotyl explants of C. arabica.…”

Section: Callogenesis and Organogenesismentioning

confidence: 78%

“…Caralluma species could not attain much attention because of difficulties in culturing, release of phenolic compounds and latex that hinder callus induction and shoot regeneration (Riaz et al 2007). Recently protocols for direct shoot regeneration from shoot tip explants of C. sarkariae (Sreelatha et al 2009;Ugrahiah et al 2011) and C. adscendens (Aruna et al 2009) were developed using MS medium enriched with plant growth regulators. There is no report of regeneration of C. tuberculata through callogenesis.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Rehman

Chaudhary

Khawar³

et al. 2014

Biologia

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Present study describes rapid in vitro propagation of Caralluma tuberculata, a traditional medicinal plant, and antioxidant potential of calli and plants extracts. The highest callus induction rate (93.3%) with maximum weight of calli 5.2 g was achieved from shoot tip explants on MS medium supplemented with 9.04 µM 2,4-D and 4.44 µM BA. The maximum shoot induction rate (71.1%) with mean number of shoots 3.66 ± 1.53 and 4.6 cm average shoot length was observed on 13.32 µM BA, 4.52 µM 2,4-D and 2.89 µM GA3 appended in MS medium. The developed shoots were best rooted in the presence of 5.07 µM IAA with 3.0 ± 0.15 roots per plantlet. The plants were successfully acclimatized under in vivo conditions. The plants and calli extracts exhibited good antioxidant activities, however, plant extract activities were more pronounced. The phenolic compounds in plant and calli extracts were 0.16% and 0.057%, respectively. While the flavonoids were 0.092% in plant and 0.039% in calli extract. Total Phenolics, flavonoids; DPPH radical scavenging activity and reducing power potential distributed among different fractions depending upon polarity of the solvent. The highest DPPH scavenging activity and reducing power was exhibited by water fractions; 4.95 mg/mL and 0.729 OD at 10 mg/mL, respectively. The micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant. Furthermore, antioxidant value describes importance of this valuable plant as food and medicine.

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“…Advantage of excellent tools of biotechnological methods for culturing plant cells and tissues offers a useful means for conservation and propagating valuable, rare and endangered medicinal species. Large numbers of medicinal plants have been successfully employed for in vitro culture technique are Fabiana imbricate [8], Thapsia garganica [9], three varieties of Caralluma adscendense [10], Caralluma stalagmifera [11] and Caralluma edulis [12]. BA is a synthetic cytokinin that deduce plant growth and development responses by stimulating cell division.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Caralluma adscendens var. fimbriata-An Indigenous Medicinal Plant of India

M¹,

J²,

Nagaraja³

et al. 2017

Nat Prod Chem Res

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Present study describes micro-propagation of traditionally important medicinal plant in northern interior part of Karnataka, Caralluma adscendens var. fimbriata, for its conservation. Results showed that the survival ability of regenerated plants was 84 per cent. Proliferation of C. adscendens var. fimbriata shoots was achieved on medium supplemented with various concentrations of different hormones. The effective combination of 6-Benzylaminopurine (BA) 2.0 mg/l along with 0.5 mg/l kinetin (Kn) and 0.2 mg/l Indole-3-acetic acid (IBA) produced highest number of shoot sprouting frequency (91%) with a mean of 2.60 ± 0.17 shoot number per explants and a mean shoot length of 3.88 ± 0.20 cm from the mature nodal explants. A good frequency of callus was induced in 1.5 to 2.0 mg/l of 1-Naphthaleneacetic acid (NAA) and in 2.0 mg/l of 2,4-dichlorophenoxy acetic acid (2, 4-D) were transferred to root induction media. The NAA showed positive response of rooting when compared with IAA and IBA in the present study. The best root and shoot sprouted plants were successfully acclimatized in the greenhouse and transferred to open field conditions. This micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant.

show abstract

Micropropagation of Three Varieties of Caralluma adscendens via Nodal Explants

Cited by 24 publications

References 9 publications

Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.

Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.

In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Micropropagation of Caralluma adscendens var. fimbriata-An Indigenous Medicinal Plant of India

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