Micropropagation of Thymus piperella

Sáez, Francisco Alberto Ruay; Sánchez, Pablo; Piqueras, Abel

doi:10.1007/bf00035981

Cited by 27 publications

(32 citation statements)

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“…Such a favorable effect of KIN was also found when three different cytokinins (BA, KIN, and 2-isopentenyl adenine) were tested for efficiency in promoting in vitro regeneration of T. vulgaris (Furmanowa and Olszowska 1992), although the authors reported that the optimum concentration was lower (0.1 mg L −1 ) than that reported here. In contrast, BA seemed to be more suitable when working with T. piperella (Sáez et al 1994), T. mastichina L. (Mendes and Romano 1999;Fraternale et al 2003) and T. sipyleus Boiss. (Baba Erdag and Yurekli 2000).…”

Section: Resultsmentioning

confidence: 68%

“…(Baba Erdag and Yurekli 2000). However, the situation was apparently different when working with T. piperella (Sáez et al 1994) and T. mastichina L. (Mendes and Romano 1999;Fraternale et al 2003), where supplementing the medium with only BA promoted better shoot growth. In the present study, the only exception to the beneficiary effect of auxins was observed when the medium was supplemented with 2, 4-D.…”

Section: Resultsmentioning

confidence: 93%

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In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots

Özüdoğru

Kaya

Kirdok

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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An efficient in vitro propagation protocol, applicable both to young and mature explants of two Thymus spp., results in genetically stable plantlets. In vitro-grown shoot tips of Thymus vulgaris L. were exposed to cytokinins (6-benzyladenine, kinetin, and thidiazuron) alone or in combination with auxins, gibberellic acid (GA 3 ) and/or silver nitrate in order to optimize in vitro shoot proliferation. Optimum shoot proliferation (97% regeneration rate, with 8.6 shoots produced per explant) was obtained when semi-solid Murashige and Skoog (MS) medium was supplemented with 1 mg L −1 kinetin and 0.3 mg L −1 GA 3 . Rooting of the shoots was easily obtained on semi-solid MS medium that was either hormone-free or supplemented with auxins. However, the best root apparatus (92.5% rooting rate, with 19 adventitious roots per shoot) developed on MS medium supplemented with 0.05 mg L −1 2,4-dichlorophenoxyacetic acid. Genetic stability was confirmed in the in vitro-germinated mother plant as well as the shoots that underwent two, four, six, eight, or ten cycles of in vitro subculturing by random amplified polymorphic DNA (RAPD) analysis. When applied to the micropropagation of mature shoot tips of T. longicaulis C. Presl subsp. longicaulis var. subisophyllus (Borbás) Jalas, the optimized in vitro propagation protocol resulted in a 97.5% shoot regeneration rate, with five shoots formed per explant, and 100% rooting. Rooted plantlets of both species were transferred to 250-mL plastic pots and successfully acclimatized by gradually reducing the relative humidity.

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 93%

In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots

Özüdoğru

Kaya

Kirdok

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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“…This circumstance has already been reported in other Lamiaceae species such as T. vulgaris (Lê, 1989, in Mendes andRomano 1999), Salvia blancoana and S. mariolensis (Cuenca and Amo-Marco 2000). T. piperella (Sáez et al 1994) required the addition of auxins alone while the combination with BA produced a negative effect. In accordance with the results of Pan and Van Staden (1998), our experiments with DPS also gave a 100 % of rooted explants, and with a better root quality than in controls, thus revealing DPS as a very interesting system for the propagation of this species.…”

Section: Rootingmentioning

confidence: 73%

“…In several species of Lamiaceae this has been the case, although not always with the same ratio. For instance, Thymus caespititius was optimally multiplied in the presence of 1 mg L -1 BA plus 0.25 mg L -1 IBA (Mendes et al 2013); in Melissa officinalis the best ratio was 1.5 mg L -1 BA and 1 mg L -1 IAA (Mészáros et al 1999); for Thymus pipperella, 1.5 mg L -1 BA and 0.5 mg L -1 IAA (Sáez et al 1994). However, Origanum vulgare required more auxin than cytokinin (0.06 mg L -1 BA and 0.1 mg L -1 NAA) for maximum multiplication (Goleniowski et al 2003).…”

Section: Shoot Proliferationmentioning

confidence: 98%

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In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Marco-Medina

Casas

2014

Plant Cell Tiss Organ Cult

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Thymus moroderi Pau ex Martínez (Lamiaceae) is a species endemic to South-eastern Spain valuable for its use in traditional medicine and in the production of a liqueur called cantueso. We describe a rapid and reliable in vitro propagation protocol for this species, starting from wild adult vegetative explants. Sterilization and establishment of in vitro culture were shown to be extremely difficult because of the high presence of contamination and the inability of non-contaminated explants to restore growth. In just two cases we were able to obtain non-contaminated explants with ability to grow: combining a fungicide dip with the presence of kanamycin in culture medium, and when mother plants were maintained for a week in a growth chamber with occasional fungicide spray. Once established in vitro, explants showed rapid and vigorous growth with spontaneous rooting. The presence of cytokinin alone or in combination with auxin gave worse results than untreated controls, thus revealing Thymus moroderi as a cytokinin-sensitive species. Better multiplication rates were however obtained when double phase system was included in the protocol. Fully acclimatized micropropagated plants were phenotypically indistinguishable from their wild relatives. Essential oils of micropropagated and wild plants were analyzed revealing a slightly different overall terpene profile. Micropropagated plants were abundant in monoterpene and sesquiterpene hydrocarbons but less in oxygenated sesquiterpenes with respect to wild plants. Major compounds were in both cases 1,8-cineole and camphor, although at different relative abundances. Despite these dissimilarities micropropagated plants produced a clearly recognizable and characteristic thyme odor.

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Correlation between the Chemical and Genetic Relationships among Thymus saturejoides Genotypes Cultured under in vitro and in vivo Environments

Nordine

Udupa²,

Iraqi³

et al. 2016

Chemistry & Biodiversity

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In this study, the in vitro and in vivo essential oil (EO) composition and genetic variability in six micropropagated genotypes of Thymus saturejoides Coss., a Mediterranean medicinal and aromatic plant, were analyzed by GC/MS and randomly amplified polymorphic DNA (RAPD). Yield and composition of the EO varied between genotypes. Cluster analysis based on RAPD data and EO grouped the six genotypes in three groups in both culture conditions, thus showing considerable intraspecific genetic and chemical variations. Applying the Mantel test, the result showed a significant correlation between the two proximity matrices RAPD and EO obtained from in vitro genotypes, whereas this correlation was not observed when using the EO obtained from the in vivo genotypes.

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Micropropagation of Thymus piperella

Cited by 27 publications

References 1 publication

In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots

In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Correlation between the Chemical and Genetic Relationships among Thymus saturejoides Genotypes Cultured under in vitro and in vivo Environments

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