Micropropagation of two endemic threatened &lt;i&gt;Cryptocoryne&lt;/i&gt; species of Sri Lanka

Herath, H. M. I.; Krishnarajah, S. A.; Wijesundara, Siril

doi:10.4038/tare.v11i0.1783

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…nana (Kanchanapoom et al, 2012), Cryptocoryne lucens (Kane et al, 1990), Cryptocoryne sp. (Herath et al, 2008), Cryptocoryne wendtii and C. becketti (Sheeja et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Aquarium Plant, Anubias Sp. ‘White’ Using Adenine Sulfate and 6-Benzylaminopurine

Boonmee,

Laohavisuti,

Srisawang

et al. 2024

J. of Law and Sust. Develop.

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Introduction: Anubias sp. “White” is distinguished from other Anubias species by mutation. The leaves are white and green, which is popular and has great market demand. The growth of this aquarium plant is slow. Method: The combination of adenine sulfate (Ads) and 6-benzyl aminopurine (BAP) into Murashige and Skoog (MS) medium. A comparison of three substrate materials, i.e., filter pads (FP), husk ash granules (HAG) and rockwool (RW) were used in ex-vitro acclimatization of the plantlets. Results: Ads and BAP had combined effects on the plantlets, leaves and roots of the apical bud (p<0.05). The treatment with only BAP at 0.5 mg/L in MS medium obtained the highest number of shoots, which was 7.40 shoots/explant (p<0.05). There is an interaction between 25 mg/L Ads and 1-1.5 mg/L BAP to induce callus. The treatments with HAG were better than the other planting materials in terms of growth performance (p<0.05). HAG yielded the best growth (fresh weight of 0.74 g/plant), which was better than FP and RW, respectively, and showed a significant (p<0.05) difference in terms of root number and plant height except root length. Conclusion: There was an interaction between 25 mg/L Ads and 1-1.5 mg/L BAP to induce callus. The treatment with only BAP at 0.5 mg/L in MS medium obtained the highest number of shoots, which was 7.40 shoots/explant (p<0.05). The HAG as a planting medium was the optimal ex-vitro acclimatization of Anubias sp. ‘white’ plantlets.

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“…nana (Kanchanapoom et al, 2012), Cryptocoryne lucens (Kane et al, 1990), Cryptocoryne sp. (Herath et al, 2008), Cryptocoryne wendtii and C. becketti (Sheeja et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Aquarium Plant, Anubias Sp. ‘White’ Using Adenine Sulfate and 6-Benzylaminopurine

Boonmee,

Laohavisuti,

Srisawang

et al. 2024

J. of Law and Sust. Develop.

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“…For in vitro rooting, shoots subjected to 1.0 mg/l NAA treatment produced an average of 36.00 ± 4.63 roots and root length at 26.02 ± 0.70 mm was significantly different from other treatments (Table 2 and Figure 2C). The use of NAA in enhancing the root formation has also been observed in Cryptocoryne beckettii and Cryptocoryne bogneri [21]; Kinnow [22] and Hippeastrum johnsonii [23].…”

Section: Effects Of Naa On Root Induction In Vitromentioning

confidence: 95%

“…The results of this study indicated that plant growth regulator supplementation is essential for the micropropagation of C. wendtii. The effectiveness of BAP in shoot bud differentiation has been documented in the number of plants [15,7]. Wang et al [16] reported that the presence of 3.0 mg/l BAP in the medium effectively increased the number of regenerated shoots of Scipus robustus an emergent hydrophyte.…”

Section: Effects Of Cytokinins On Direct Shoot Organogenesis Induction From Shoot Tip Explants In Vitromentioning

confidence: 99%

Induction of Direct Shoot Organogenesis from Shoot Tip Explants of an Ornamental Aquatic Plant, Cryptocoryne wendtii

Klaocheed

Jehsu

Choojun

et al. 2018

Walailak J Sci & Tech

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Cryptocoryne wendtii is an important amphibious species with a wide range of foliage colors. Although it has a high market demand, the natural propagation of its aquatic species is limited due to the limited production on the number of plants with a long cultivation period, disease, and the requirement for a large space for propagation. Thus, we studied the effects of the plant growth regulators and their concentrations on the induction of direct shoot organogenesis from shoot tip explants of Cryptocoryne wendtii. The shoot tips were sterilized on its surface using 8 % Clorox® (5.25 % sodium hypochlorite, NaOCl) for 15 min followed by rinsing them three times with sterile distilled water. They were again sterilized on the surface for another 4 % Clorox® (5.25 % sodium hypochlorite, NaOCl) for 5 min. Cytokinins played a crucial role in direct shoot organogenesis. Direct shoot organogenesis from shoot tip explants was promoted by incubating these explants on Murashige and Skoog (MS) medium [1] in the presence of two different cytokinins [6-benzyl-aminopurine (BAP) or Kinetin (Kin)], each provided at four different levels. Direct shoot organogenesis was induced in both explants. Shooting occurred 100 % from in vitro shoot tip explants, which was cultured on MS medium and supplemented with 3.0 mg/l BAP. This was significantly different from the other treatments with the highest number of 16.20 shoots per explant and number of leaves at 72.40 leaves per explant after 60 days of culture. Individual shoots, aseptically excised, which produced normal roots within 45 days on the MS medium supplemented with α-Naphthaleneacetic acid (NAA). The highest number of roots per shoot and the longest roots were obtained on MS medium supplemented with 1.0 mg/l NAA (100 % rooting, which was an average of 36 roots per plantlet and root length at 26.02 mm). Rooted plantlets were successfully hardened and established in pots containing a mixture of organic soil and sand (1:1) overlaid with tap water under greenhouse conditions at 90 % survival. This complete study has successfully outlined a rapid, high frequency direct shoot organogenesis induction of an ornamental aquatic plant, Cryptocoryne wendtii from shoot tip explants inclusive of shoot proliferation, rooting and acclimatization. The present in vitro propagation protocol would facilitate an alternative method for rapid, large-scale production and germplasm preservation of this important endangered species C. wendtii.

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Research and development and innovations in floriculture: lessons from the market giants for developing countries like Sri Lanka

Beneragama¹,

Peiris²

2016

Acta Hortic.

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Micropropagation of two endemic threatened <i>Cryptocoryne</i> species of Sri Lanka

Cited by 6 publications

References 7 publications

Micropropagation of Aquarium Plant, Anubias Sp. ‘White’ Using Adenine Sulfate and 6-Benzylaminopurine

Micropropagation of Aquarium Plant, Anubias Sp. ‘White’ Using Adenine Sulfate and 6-Benzylaminopurine

Induction of Direct Shoot Organogenesis from Shoot Tip Explants of an Ornamental Aquatic Plant, Cryptocoryne wendtii

Research and development and innovations in floriculture: lessons from the market giants for developing countries like Sri Lanka

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