Micropropagation of Woody Plants

Rathore, Jitendra Singh; Rathore, Vinod; Shekhawat, N. S.; Singh, Rakhi; Liler, G.; Phulwaria, Mahendra; Dagla, Harchand R.

doi:10.1007/1-4020-3213-7_13

Cited by 20 publications

(15 citation statements)

References 35 publications

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“…Juvenile explants are often used for micropropagation of woody species due to the difficulties encountered when explants from adult plants are used for cloning (Rathore et al 2004). However, juvenile plants are usually of unknown genotype, making it difficult to clone adult selected trees (Hackett 1985).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Gomes

Canhoto

2008

In Vitro Cell.Dev.Biol.-Plant

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Arbutus unedo L. is a species of strawberry tree, widely represented in the Mediterranean climates of southern Europe. Fruits are used to make jellies and a spirit called "medronheira." Shoot apices and nodal segments from epicormic and coppiced shoots of adult plants were used for plant propagation. Shoot apices from epicormic shoots, which were developed in a growth chamber, showed higher rates of in vitro establishment. The results also indicated that shoot apices are more effective for plant establishment than nodal segments, with rates of establishment significantly higher after 12 wk of culture. Of the three basal media used in combination with 9.0 µM benzyladenine and 0.087 M sucrose, the FS medium with the micronutrients of the Murashige and Skoog medium gave the highest rates of multiplication, especially when the parameter analyzed was the number of clusters formed. When shoot apices from selected adult plants (AL01-AL06) were tested, the multiplication rate was not significantly different among the plants. However, in the conditions tested, shoots from the clones AL1, AL2, and AL3 showed better development, whereas shoots from AL4, AL5, and AL6 showed an impaired development and could not be rooted. Rooting was achieved in all the conditions tested, even in the absence of auxin. The inclusion of an auxin significantly increased root formation, whereas the addition of charcoal did not improve root formation. Rooted plantlets were acclimatized, and some of them are now in the field for further study.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Gomes

Canhoto

2008

In Vitro Cell.Dev.Biol.-Plant

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“…The application of tissue culture methods for vegetative propagation of temperate fruit rootstocks started in the mid-70s, and a considerable number of improved protocols were developed ever since. Generally, the goal of micropropagation is obtaining rapid, large-scale and low-cost production of genetically identical, physiologically uniform and pathogen-free plants (Rathore et al 2004). …”

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confidence: 99%

In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks

Vujović¹,

Ružić²,

Cerović³

2012

Hortic. Sci.

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Vujović T., Ružić Dj., Cerović R., 2012. In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks. Hort. Sci. (Prague), 39: 101-107.In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf ) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.

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“…These have more vigor to tolerate stresses experienced during hardening condition (Pospsilovia et al 1999;Yan et al 2010;Shekhawat et al 2012). Effectiveness of IBA as compared to other auxins on ex-vitro rooting has also been reported earlier in many woody tree species (Rathore et al 2005;Arora et al 2010;Phulwaria et al 2012a). After 25-30 days of acclimatization, plantlets were successfully transferred from bottles to black poly-bag containing sand, clay soil, and organic manure.…”

mentioning

confidence: 78%

Use of liquid culture medium and ex vitro rooting for micropropagation of Acacia nilotica (L.) Del. ssp. cupressiformis

Rathore

Phulwaria

Manoj

et al. 2015

Ind J Plant Physiol.

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A micro-propagation method using liquid culture medium has been developed for Acacia nilotica ssp. cupressiformis. Nodal segments obtained from mature tree (15-20 years old) was used as explants for bud breaking and multiple shoot induction. MS liquid medium with 2.0 mg l -1 of 6-benzylaminopurine (BAP) was the best for shoot bud induction. Further shoots multiplication was achieved by subculturing of in vitro produced shoot clumps (2-3 shoots) in liquid medium supplemented with various concentrations of BAP. Maximum number of shoots (12.57 ± 0.37) and shoot length (10.11 ± 0.42) were recorded in MS liquid medium supplemented with 1.0 mg l -1 of BAP. About 90 % shoots rooted ex-vitro on soilrite within 3-4 weeks when base (3-5 mm) of shoots was treated with 300 mg l -1 of indole-3-butyric acid for 5 min. The plantlets were hardened successfully in the green house and transferred to the polybags. To the best of our knowledge, this is the first report of a micro-propagation protocol for A. nilotica ssp. cupressiformis.

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Micropropagation of Woody Plants

Cited by 20 publications

References 35 publications

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

Micropropagation of strawberry tree (Arbutus unedo L.) from adult plants

In vitro shoot multiplication as influenced by repeated subculturing of shoots of contemporary fruit rootstocks

Use of liquid culture medium and ex vitro rooting for micropropagation of Acacia nilotica (L.) Del. ssp. cupressiformis

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