Micropropagation of Yucca Plant by Using Guar and Locust Bean Seed Powder as an Alternative Cheap Gelling Agent

Hassan, Heba A.; Moubarak, Manee

doi:10.21608/sjfop.2020.110364

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…Alawaadh et al (2020) on Philodendron bipinnatifidum stated that adding BAP at 1 mg/l significantly increased shoot multiplication compared with other cytokinins. Hassan and Moubarak (2020) declared that the results of MS medium supplemented with 1 mg/l BA significantly outperformed other tested cytokinins, yucca shoot multiplication, and growth parameters (number of shoots and leaves/shoot, and shoot length).…”

Section: Multiplication Stagementioning

confidence: 99%

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

Sayed¹

2021

Scient. J. of Flowers and Ornament. Plants

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during 2018 and 2019 seasons, with the aim to determine the best protocol of tissue culture technology to propagate Dracaena umbraculifera plant. For the possibility of spreading its cultivation to preserve it from extinction. As this plant is found in very few numbers, and it is difficult to form seeds under the climatic conditions in Egypt. Results obtained stated that using Clorox at 40% for 20 min gave the highest significant survival percentage (86.13%). For multiplication stage, benzyl adenine (BA) proved its superiority over kinetin in the production of a greater number and length of shoots. The highest number of shoots (3.20) was obtained with BA at 1.5 ppm and the highest shoots length (2.23 cm) was recorded with BA at 1.3 ppm. For rooting stage, the use of 1-naphthaleneacetic acid (NAA) at 0.3 ppm produced the highest values of roots number/plantlet (3.13) and root length (5.93 cm), while the lowest values were recorded when using indole-3-butyric acid (IBA) at 0.1 ppm. For acclimatization stage, the type of pot media had a significant effect. Using peat moss only under greenhouse condition gave the highest survival percentage (100%) and the highest values for all the measurements such as plant height (cm), number of leaves/plant, root length (cm), number of roots/plant, fresh and dry weights of leaves and roots (g/plant). Likewise, for the content of the leaves of total chlorophyll, carotenoids and the percentage of total soluble sugars. So, it can be recommended to make a protocol by using the tissue culture technique for propagating of Dracaena umbraculifera plant as follows: use Clorox at 40% for explant sterilization, using 1.5 ppm of BA in the multiplication stage, using 0.3 ppm of NAA in the rooting stage and using peat moss only to grow dracaena plant in the acclimatization stage to get plants with good growth characteristics.

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Section: Multiplication Stagementioning

confidence: 99%

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

Sayed¹

2021

Scient. J. of Flowers and Ornament. Plants

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“…It was expensive and overexploited when agar was predominantly used as the gelling agent for microbial and plant cell culture media (Das et al 2019). Others have voiced concerns regarding agar applicability, especially for its limited resources overuse, and considerations include exorbitant pricing (Hassan and Moubarak 2020;Hegele et al 2021). Previous studies have investigated the use of Vol:.…”

Section: Mechanical Strengthmentioning

confidence: 99%

“…In an effort to reduce the cost of commercial micropropagation, other alternatives, such as methylcellulose, potato and corn starches, alginate, gellan gum, gelatin, pectin, and microcrystal cellulose have been used instead of agar (Bornman and Vogelmann 1984;Calleberg et al 1989;Gorinova et al 1993;Hassan and Moubarak 2020;Mbanaso and Roscoe 1982).…”

Section: Introductionmentioning

confidence: 99%

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

et al. 2022

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Bacterial cellulose (BC) is a natural biodegradable, eco-friendly fiber, lying within the nanoscale range. It is reputable for its various physical and chemical qualities, like high hydrophilicity, immense crystallinity, ease of sterility, being toxin-free, and extremely pure. Adding to its wide applicability in different fields, this study evaluated the applicability of a developed gelling substitute for plant tissue culture media. The BC matrix was characterized under the acronym PLATIBACGEL (PLAnt TIssue Culture BActerial Cellulose GEL), formed by Komagataeibacter hansenii AS.5, preisolated from rotten apple waste. Scanning electron microscope, Fourier-transform infrared, X-ray diffractometer, and tensile strength analyses confirmed the formation of purified, porous, and heterogeneous densely packed multiple network polymers possessing cellulose properties. The water holding capacity (WHC) values of wet and dried BC membranes were 9179% and 226.9%, respectively, and the water absorption rate (WAR) of dry BC membranes was higher than that of wet membranes. Using BC as a tissue culture gelling agent, six genotypes from tomato and wheat seeds were cultured in vitro, for guaranteeing explant genetic diversity, over seven treatments. Treatment 5, included PLATIBACGEL as the main constituent, improved and sustained all in vitro seed germination, root penetration, and plant support. Likewise, repeated tomato micropropagation subcultures were successful. Results demonstrated applying PLATIBACGEL as a promising, reusable, cheap, and reliable alternative plant micropropagation media gelling agent. Wherefore, plant cellular developers and tissue-culturists can utilize bio-polymers like BC for better understanding plant cell response to different in vitro culturing conditions, with expected beneficial returns on gelling agents industry and markets as well. Graphical abstract

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