The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readilyavailable alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters [number of shoots/explant, number of leaves/shoot, and shoot length (cm)] which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v).

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