Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Shoyama, Yukihiro; Zhu, Xuan Xuan; Nakai, Rie; Shiraishi, Shinya; Kohda, Hiroshi

doi:10.1007/bf01092764

Cited by 21 publications

(3 citation statements)

References 23 publications

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“…Moreover, it is possible to study a large number of herbal medicines containing white ginseng for quality control purposes, and also regenerated plantlets cultured in the laboratory. 36,37 Kikyo-to 0+5 -a Composition ratio expresses the contents of ginseng (g) used in each prescription. b The content of G-Rb1 was calculated from the composition of ginseng and the standardized G-Rb1 content (5.49 mg g 21 dry weight) determined by ELISA as indicated in Table 2.…”

Section: Quantitative and Qualitative Analysis Of Ginsenosides By Wes...mentioning

confidence: 99%

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Fukuda

Tanaka

Shoyama

2000

Analyst

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A combination of ELISA, Western blotting and immunoaffinity concentration using an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitative and quantitative surveys of ginsenoside Rb1 and related ginsenosides in roots and traditional Chinese herbal medicines. To improve the low correlation between ELISA and HPLC analysis, the crude extract of roots was immunoaffinity concentrated to make evident the effect of malonyl ginsenoside Rb1. Immunoaffinity column chromatography also concentrated an unknown ginsenoside which had the same cross-reaction with ginsenoside Rb1.

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Section: Quantitative and Qualitative Analysis Of Ginsenosides By Wes...mentioning

confidence: 99%

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Fukuda

Tanaka

Shoyama

2000

Analyst

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“…There is a very high global demand for this species due to its antihypertensive, antithrombotic, anti-atherosclerotic, anti-tumor, anti-oxidant, and hepatoprotective activities ( Zhou et al, 2009 ; Yang et al, 2015 ; Dong et al, 2019 ; Li et al, 2019 ; Liu et al, 2019 ; Zhang et al, 2019a ; Tang et al, 2020 ). Recently, more than 4 million hectares of land are cultivated annually in China for this species ( Shoyama et al, 1997 ; Deborah et al, 2005 ; Wei et al, 2015 ). After 1–3 years of cropping, root rot is prevalent in this species ( Sun et al, 2015 ; Yang et al, 2015 ; Zhang et al, 2019b ).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis on the Structure and Function of the Panax notoginseng Rhizosphere Microbiome

Kui

Chen

et al. 2021

Front. Microbiol.

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Panax notoginseng, an important Chinese medicinal herb, can be mainly cultivated in two planting patterns, cropland planting (DT) and understory planting (LX). We speculate that the rhizosphere microbiome may vary in DT and LX and may play an important role in promoting the growth and health of P. notoginseng. In the present study, culture-independent Illumina HiSeq was employed to investigate the rhizosphere bacteria and fungi under DT and LX planting patterns. Predominant phyla include Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, and Ascomycota in the two planting patterns. DT has higher alpha diversity index than LX. The predominant LX-core genera include Bradyrhizobium, Streptomyces, and Actinomadura, and the predominant DT-core genera include Sphingomonas, Variovorax, and Novosphingobium. Total relative abundance of the disease-suppression phylum (Proteobacteria, Firmicutes, and Actinobacteria) and the potential plant growth-promoting rhizobacteria (PGPR) were both significantly higher in LX than in DT. We also identified over-presented microbial functional traits mediating plant–microbe and microbe–microbe interactions, nutrition acquisition, and plant growth promotion in P. notoginseng rhizosphere. Our findings provide a valuable reference for studying beneficial microbes and pathogens of P. notoginseng planted in DT and LX.

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“…We have succeeded in the isolation of ginsenoside Rb1 from a different plant, Kalopanax pictus Nakai, which was not known previously to contain ginsenosides, using this combination of methods (data not shown). This combination procedure has also been used in a breeding program of P. ginseng using tissue culture techniques, , resulting in a plant yielding higher ginsenoside Rb1 concentrations.…”

mentioning

confidence: 99%

Isolation of the Pharmacologically Active Saponin Ginsenoside Rb1 from Ginseng by Immunoaffinity Column Chromatography

2000

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Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Cited by 21 publications

References 23 publications

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

A Comparative Analysis on the Structure and Function of the Panax notoginseng Rhizosphere Microbiome

Isolation of the Pharmacologically Active Saponin Ginsenoside Rb1 from Ginseng by Immunoaffinity Column Chromatography

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