Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

Abstract

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

Effect of Cutting Size on the Performance of Exogenous Auxin IBA in Vegetative Propagation of Rubus erythroclados Mart. ex Hook. f.

“…Currently, this species is described as an unconventional food plant due to its green fruits with high sugar levels and pleasant taste [3,4]. R. erythroclados has the potential to be among the main small fruits grown in Brazil together with blackberry and raspberry because of the quality of its fruits [5].…”

Micropropagation on multiplication of native greenberries in South of Brazil

“…are commonly known as whiteberries or greenberries, alluding to the color of the fruits that remain green even when ripe (Bueno, 2015). The fruits have high levels of sugar and a pleasant peculiar flavor, which gives market potential compared to other small fruits (Bueno, Biasi, & Tofanelli, 2018). Information about the ethno-knowledge of these species in the Santa Catarina South Plateau indicates that although they are much appreciated for their sweet taste and were less acidity than blackberries their consumption is made only in fresh fruits mode, when the fruits are found at random, without the habit of collection for processing (Couto Waltrich, Boff & Boff, 2017).…”

“…For greenberries, vegetative propagation studies indicate that multiplication via cuttings is inefficient, whereas in vitro cultivation requires adjustments and further studies (Bueno, 2015). The in vitro propagation of R. erythroclados was evaluated by Bueno et al (2018) who found low rooting rates and paralyzed studies, which highlights the necessity of retake work on development of specific protocols for these species. Micropropagation can provide better seedling uniformity and vigor, increased mass production and better health (Dutra, et al, 2010), in addition to allowing molecular biology work to conserve plant genetic resources (Bakhtiar, Mirjalili, & Sonboli, 2016).…”

Regeneración y enraizamiento de brotes adventicios etiolados de cultivares de zarzamora (Rubus sp)

“…Asimismo, los reguladores de crecimiento empleados para inducir una respuesta morfogénica en explantes de Rubus incluye a citocininas y auxinas (Bueno et al, 2018), citocininas y giberelinas (Cancino-Escalante et al, 2015;Millones, 2018;Pérez-Martínez Castañeda-Garzón, 2016). Además, para la elongación de los brotes de R. ideaus ha sido empleado brasinolido, hormona vegetal de tipo esteroidal (Robres-Torres et al, 2015).…”