Micropropagation Technology in Early Phases of Commercial Seed Potato Production

Abstract

^MoS^SO^ Propositions 1. The seed potato industry in the province of Alberta (Canada) will be enhanced by the introduction of microtubers in the seed production system. (this thesis)2. Jasmonates stimulate the production of potato microtubers and their performance in the greenhouse production of minitubers. (this thesis)3. Genotypic responses to in vitro conditions may determine the success or failure of the introduction of the method to a new cultivar.4. GMO potato can be a public enemy or a friend.5. The Dutch seed potato system helped the potato to colonize the world.6. The small fruit industry in Alberta should be linked closer to the seed potato industry for mutual economic benefits.7. Micropropagation is blooming in China and can significantly change the economy of the Three Gorges Region on Yangtse River in China.8. Organic agriculture began as an ideology, but it cannot meet today's needs.Propositions belonging to the PhD thesis of Krzysztof Pruski Micropropagation technology in early phases of commercial seed potato production Wageningen, 31 October 2001. AbstractPruski, Krzysztof, 2001. Micropropagation technology in early phases of commercial seed potato production. PhD thesis, Wageningen University and Research Centre, Wageningen, The Netherlands, 166 pp., English and Dutch summaries.Micropropagation (in vitro propagation) has been introduced to seed potato production programmes more than two decades ago. The research reported in this thesis studied possibilities of improvements to micropropagation methods commonly used in commercial laboratories. The focus was placed on seed potato growers in Western Canada who either produce the tissue culture plant material themselves and use it for the production of minitubers (nuclear tubers) in their own operations, or acquire it prior to planting. The autotrophic micropropagation (in C0 2 (1500 ppm) enriched atmosphere, no sucrose in the medium) of Russet Burbank variety showed its usefulness for the small commercial laboratories where often the full sterility is difficult to maintain. Autotrophically grown cultures produced stems very similar to conventionally grown in vitro cultures (no significant differences in length, number of nodes and dry weight). Also, the conventionally propagated cultures benefited from CO2 enrichment during the 4 week growing period by a 20% increase in the number of nodes per stem and a 50% increase in stem dry weight (doubled stem length).The use of continuous low red light (690 nm) at 3 uxnol nf 2 s~l PPFD and 30 g f~' sucrose in the medium during the in vitro low temperature (4°C) storage of potato cultures were beneficial for maintenance of vigorous, high quality cultures with a high re-grow capacity. In small tissue culture laboratories, installation of a simple low light device in the regular refrigerator would improve maintenance of the high quality of the stored cultures.Significantly better production of microtubers (number of tubers and weight) was observed on solid (agar) than on liquid media, and under the 8 h photoper...

Agricultural Interventions to Enhance Climate Change Adaptation of Underutilized Root and Tuber Crops

“…This method is very important for plant propagation for example the use of this method in seed production has ensued in mass production of clean potato seeds within a short period of time. In support of this, Pruski (2001) revealed that the method is characterized by its flexibility in terms of the rapid multiplication of seedlings. This method has been adopted in several countries like Vietnam where it revolutionized production of potato seeds.…”

In vivo performance of hi-tech planting materials for seed potato production: A review

“…Use of tissue culture technique in seed production has resulted in mass production of potato in a very short period of time. The system is characterized by very flexible and rapid multiplication giving a higher planting propogules (Beukema and Van de Zaag 1990 [4] and Pruski, 2001 [36] , Muthuraj et al, 2016 [26] ). Slowly, micro propagation is replacing conventional propagation of potatoes (Naik and Karihaloo, 2007 [29] , Moeini et al, 2011 [25] ).…”

Evalution of possibilities to increase efficiency of potato minituber production = Kartupeļu sīkbumbuļu audzēšanas efektivitātes paaugstināšanas iespēju izvērtējums

“…Sīkbumbuļi. Sākotnējās sēklaudzēšanas mērķis ir pēc iespējas ātrāk un ekonomiski izdevīgāk saražot izejmateriālu sēklaudzēšanas saimniecībām un sīkbumbuļu audzēšana ļauj samazināt lauka paaudžu skaitu (Struik, Lommen, 1999;Struik, Wiersema, 1999 (Kotkas, Rosenberg, 1999;Pruski, 2001;Haapala, 2005;Kawakami, Oohori, Tajima, 2015). Tomēr literatūrā atrodami atšķirīgi uzskati par to, vai mātesaugi kā izejmateriāls sēklaudzēšanai būtu jāiegūst katru gadu no jauna (no meristēmām, dzinumu galotnēm vai citādi), vai tomēr var izmantot gēnu bankās un repozitorijos esošo materiālu, kur tas tiek ilgstoši uzglabāts.…”

“…Kartupeļu mikropavairošanā tiek lietoti arī lielāki in vitro augu audzēšanas trauki, īpaši pēdējā pavairošanas fāzē. Literatūrā minētas 300 mL tilpuma kolbas (Correa et al, 2008), plastmasas konteineri, kuros iepilda 100 mL barotnes (Milinkovic et al, 2012), 5 cm augsti plastmasas trauki, kuru diametrs ir 10 cm un kuros iepildīti 75 mL barotnes , Magenta korporācijā ASV ražotie trauki GA7 (Pruski, 2001) un minēti arī "piemēroti trauki" (Dodds, 1988). Vienā traukā audzēti no 10 līdz pat 25 kartupeļu mikroaugiem.…”

“…Sīkbumbuļu uzglabāšana. Sīkbumbuļus parasti uzglabā 2-4 °C temperatūrā (Pruski, 2001;Lommen, 1993a,b;Struik, Lommen, 1999;Rykaczewska, 2016a), ko sasniedz, temperatūru pakāpeniski pazeminot. Ja glabātuvē nodrošināti labvēlīgi apstākļi (relatīvais mitrums ~80%), tad sīkbumbuļus var uzglabāt līdz pat 18 mēnešiem, tomēr daļai genotipu var pasliktināties dīdzība un arī sekojošā raža.…”