Micropterus salmoides rhabdovirus (MSRV) infection induced apoptosis and activated interferon signaling pathway in largemouth bass skin cells

Gao, E-Bin; Chen, Guifang

doi:10.1016/j.fsi.2018.03.008

Cited by 50 publications

(16 citation statements)

References 44 publications

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“…The MSRV was not restricted to a specific cell line. In addition to causing CPE in epithelioma papillosum of carp (EPC), Chinese perch brain (CPB), fathead minnow (FHM), and largemouth bass skin (LBS) cell lines (Dorson et al, 1984;Fu et al, 2017;Gao and Chen, 2018), MSRV-YH01 successfully infected the GCO cell line and resulted in obvious CPE. Unfortunately, we did not see the representative bullet-shaped morphology of rhabdovirus by TEM.…”

Section: Discussionmentioning

confidence: 99%

“…As a commercial freshwater species, largemouth bass (Micropterus salmoides) has been introduced to various countries including China and cultured widely (Lorenzoni et al, 2002;Gao and Chen, 2018;Zhang LJ et al, 2018). In recent years, the annual production of M. salmoides has increased dramati-cally to reach 456 888 metric tons in China in 2017 .…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, both wild and cultured M. salmoides encounter lethal and epidemic diseases caused by viruses, parasites, and bacteria due to immoderate culture and environmental contamination (Bauer, 2013;Ma et al, 2013;Ho et al, 2018). Among these diseases, the frequent outbreak of M. salmoides rhabdovirus (MSRV) is of particular concern because it is lethal to juveniles (Gao and Chen, 2018). MSRV was first discovered by Ma in April 2011 at a farm in Zhongshan City, Guangdong Province, China (Ma et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Lyu

Yuan

Zhang

et al. 2019

J. Zhejiang Univ. Sci. B

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As one of the most important aquatic fish, Micropterus salmoides suffers lethal and epidemic disease caused by rhabdovirus at the juvenile stage. In this study, a new strain of M. salmoides rhabdovirus (MSRV) was isolated from Yuhang, Zhejiang Province, China, and named MSRV-YH01. The virus infected the grass carp ovary (GCO) cell line and displayed virion particles with atypical bullet shape, 300-500 nm in length and 100-200 nm in diameter under transmission electron microscopy. The complete genome sequence of this isolate was determined to include 11 526 nucleotides and to encode five classical structural proteins. The construction of the phylogenetic tree indicated that this new isolate is clustered into the Vesiculovirus genus and most closely related to the Siniperca chuatsi rhabdovirus. To explore the potential for a vaccine against MSRV, a glycoprotein (1-458 amino acid residues) of MSRV-YH01 was successfully amplified and cloned into the plasmid pFastBac1. The high-purity recombinant bacmid-glycoprotein was obtained from DH10Bac through screening and identification. Based on polymerase chain reaction (PCR), western blot, and immunofluorescence assay, recombinant virus, including the MSRV-YH01 glycoprotein gene, was produced by transfection of SF9 cells using the pFastBac1-gE2, and then repeatedly amplified to express the glycoprotein protein. We anticipate that this recombinant bacmid system could be used to challenge the silkworm and develop a corresponding oral vaccine for fish.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Lyu

Yuan

Zhang

et al. 2019

J. Zhejiang Univ. Sci. B

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“…The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, United States) and then sequenced on the Illumina sequencing platform (HiSeqTM 2500). After the initial assembly, the differentially expressed genes (DEGs) related to UPS was analyzed as described previously ( Gao and Chen, 2018 ). The changes of target genes were analyzed using the expression levels in SGIV-infected cells compared to those in mock-infected cells at indicated time points.…”

Section: Methodsmentioning

confidence: 99%

Ubiquitin–Proteasome System Is Required for Efficient Replication of Singapore Grouper Iridovirus

et al. 2018

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The ubiquitin–proteasome system (UPS) serves as the major intracellular pathway for protein degradation and plays crucial roles in several cellular processes. However, little is known about the potential actions of the UPS during fish virus infection. In this study, we elucidated the possible roles of UPS in the life cycle of Singapore grouper iridovirus (SGIV); a large DNA virus that usually causes serious systemic diseases with high mortality in groupers. Data from transcriptomic analysis of differentially expressed genes illustrated that expression of 65 genes within the UPS pathway, including ubiquitin encoding, ubiquitination, deubiquitination, and proteasome, were up- or down-regulated during SGIV infection. Using different proteasome inhibitors, inhibition of the proteasome decreased SGIV replication in vitro, accompanied by inhibition of virus assembly site formation, and viral gene transcription and protein transportation. Over-expression of ubiquitin partly rescued the inhibitory effect of ubiquitin inhibitor on SGIV replication, suggesting that UPS was required for fish iridovirus infection in vitro. Viral or host proteins regulated by proteasome inhibition during SGIV infection were investigated with two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sixty-two differentially expressed proteins, including 15 viral and 47 host proteins, were identified after SGIV infection. The host proteins were involved in ubiquitin-mediated protein degradation, metabolism, cytoskeleton, macromolecular biosynthesis, and signal transduction. Among them, 11 proteins were negatively regulated upon MG132 treatment during SGIV infection. This is believed to be the first study to provide evidence that UPS was essential for fish virus infection and replication.

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“…As one kind of important viral pathogens, fish rhabdoviruses could infect several kinds of fish and activate the typical programmed cellular death pathway of fish cells [ 10 ]. Present studies indicated that fish rhabdoviruses could induce apoptosis and autophagy by activating several signaling pathways in different cell types [ 11 , 12 , 13 ]. Du et al [ 14 ] found that one strain of fish rhabdovirus, Scophthalmus maximus rhabdovirus (SMRV), could activate caspase-mediated apoptosis in carp leucocyte cells, while another fish rhabdovirus, spring viraemia of carp virus (SVCV), can utilize the autophagy pathway activated by its glycoprotein to facilitate own genomic RNA replication and enhance its titers in the supernatants of epithelioma papulosum cyprini (EPC) cells [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Autophagy Delays Apoptotic Cell Death Induced by Siniperca chuatsi Rhabdovirus in Epithelioma Papulosum Cyprinid Cells

Zhou

Sun

et al. 2021

Viruses

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Autophagy and apoptosis are two key cell fate determination pathways, which play vital roles in the interaction between viruses and host cells. Previous research had confirmed that one strain of fish rhabdoviruses, Siniperca chuatsi rhabdovirus (SCRV), could induce apoptosis and autophagy after infection. In the current study, we continued to analyze the interaction of autophagy and apoptosis in SCRV-infected EPC cell lines after treatment with different autophagy or apoptosis inhibitors. We found that SCRV infection could activate the mitochondrial apoptotic pathway by the detection of the activities of the caspase-3 and caspase-9 and by flow cytometry analysis in JC-1-stained cells, respectively. Furthermore, no significant autophagy-related factors were disturbed in SCRV-infected cell after apoptosis inhibitor Z-VAD-FMK treatment, while autophagy inducer rapamycin could obviously delay the occurrence of CPE and cell death. Meanwhile, rapamycin was able to reduce the proportion of apoptotic cells. Besides that, rapamycin could disturb the expression of p62 and LC3B-II, and the transcription level of SCRV nucleoprotein mRNA. The progeny virus titers did not show a big difference between the rapamycin treatment or without it. Collectively, our data preliminarily confirmed that SCRV-activated autophagy could delay apoptosis in EPC cells and may not affect virus production. Further study may need to focus on the crosstalk regulation and its roles on the SCRV infection.

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Micropterus salmoides rhabdovirus (MSRV) infection induced apoptosis and activated interferon signaling pathway in largemouth bass skin cells

Cited by 50 publications

References 44 publications

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Isolation and characterization of a novel strain (YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

Ubiquitin–Proteasome System Is Required for Efficient Replication of Singapore Grouper Iridovirus

Autophagy Delays Apoptotic Cell Death Induced by Siniperca chuatsi Rhabdovirus in Epithelioma Papulosum Cyprinid Cells

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