MicroRNA-101, mitogen-activated protein kinases and mitogen-activated protein kinases phosphatase-1 in systemic lupus erythematosus

Yang, Juan; Yw, Lu; Mm, Lu; Rx, Leng; Hf, Pan; Dq, Ye

doi:10.1177/0961203312465779

Cited by 13 publications

(15 citation statements)

References 43 publications

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“…17,18 Previous studies demonstrated that expression of miR-101a is modulated by the PI3K/Akt signaling pathway, which is activated in response to IL-1β. 19,20 Wei et al 21 have demonstrated that miR-30b is positively regulated by NF-κB. Therefore, we believe that IL-1β alone is capable of inducing alterations in miR-101a and miR-30b expression, and that IL-1β-mediated signaling pathways participate in the induction of miR-101a and miR-30b in β-cells, though the latter hypothesis requires further investigation.…”

Section: Discussionmentioning

confidence: 75%

miR-101a and miR-30b contribute to inflammatory cytokine-mediated β-cell dysfunction

Zheng

Wang

et al. 2015

Laboratory Investigation

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Inflammatory cytokines have a critical role in the progressive deterioration of pancreatic β-cell function and development of type 1 diabetes. Prolonged exposure of β-cells to inflammatory cytokines results in gene expression modifications, leading to loss of β-cell function. MicroRNAs (miRNAs) are small non-coding RNAs acting as key regulators of gene expression. Here, we demonstrate that miR-101a and miR-30b are key players in cytokine-mediated β-cell dysfunction. We found that IL-1β induces an increase in miR-101a and miR-30b in MIN6 cells, and that the two miRNAs participate in β-cell dysfunction, including decreased insulin content, gene expression, and increased β-cell death. miR-101a and miR-30b reduce proinsulin expression and insulin content by directly targeting the transcriptional factor Neurod1. In addition, β-cell apoptosis mediated by miR-101a and miR-30b is associated with diminished expression level of the antiapoptotic protein Bcl2. Moreover, we show that miR-101a causes an impairment in glucose-induced insulin secretion by decreasing the expression of the transcription factor Onecut2. Taken together, our findings suggest that changes in the levels of miR-101a and miR-30b contribute to cytokine-mediated β-cell dysfunction occurring during the development and progression of type 1 diabetes.

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Section: Discussionmentioning

confidence: 75%

miR-101a and miR-30b contribute to inflammatory cytokine-mediated β-cell dysfunction

Zheng

Wang

et al. 2015

Laboratory Investigation

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“…KEGG analysis suggests that seven of nine genes of the MAPK signaling pathway are target genes of miRNA-101-3p: CACNB2, DUSP1, FOS, NLK, RAC1, RAP1B and TGFBR1. Regulation of the miR-101/MAPK pathway plays an important role in proliferation and metastasis of gallbladder carcinoma [47], in LPS-activated Cellular Physiology and Biochemistry Cellular Physiology and Biochemistry macrophages [48], in systemic lupus erythematosus [49] and in proliferation and migration of cardiac fibroblast [50]. Wang et al found that inhibition of miR-101could attenuate H9C2 apoptosis induced by hydrogen peroxide [41].…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine

Yang

Chen

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine.

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“…A number of studies have indicated that miR-101 is involved in a variety of diseases [ 40 , 41 , 42 , 43 , 44 ]. Recently, we investigated the miRNA expression profiling in the MG-infected lungs vs. the non-infected lungs of specific-pathogen-free (SPF) chicken embryos by Solexa deep sequencing (lab unpublished data).…”

Section: Introductionmentioning

confidence: 99%

gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken

Chen

Wang

et al. 2015

IJMS

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Mycoplasma gallisepticum (MG), one of the most pathogenic Mycoplasma, has caused tremendous economic loss in the poultry industry. Recently, increasing evidence has suggested that micro ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about potential roles of miRNAs in MG infection of chicken. In the present study, using miRNA Solexa sequencing we have found that gga-miR-101-3p was up-regulated in the lungs of MG-infected chicken embryos. Moreover, gga-miR-101-3p regulated expression of the host enhancer of zeste homolog 2 (EZH2) through binding to the 3’ un-translated region (3’-UTR) of EZH2 gene. Over-expression of gga-miR-101-3p significantly inhibited EZH2 expression and hence inhibited proliferation of chicken embryonic fibroblast (DF-1 cells) by blocking the G1-to-S phase transition. Similar results were obtained in MG-infected chicken embryos and DF-1 cells, where gga-miR-101-3p was significantly up-regulated, while EZH2 was significantly down-regulated. This study reveals that gga-miR-101-3p plays an important role in MG infection through regulation of EZH2 expression and provides a new insight into the mechanisms of MG pathogenesis.

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MicroRNA-101, mitogen-activated protein kinases and mitogen-activated protein kinases phosphatase-1 in systemic lupus erythematosus

Cited by 13 publications

References 43 publications

miR-101a and miR-30b contribute to inflammatory cytokine-mediated β-cell dysfunction

miR-101a and miR-30b contribute to inflammatory cytokine-mediated β-cell dysfunction

Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine

gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken

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