MicroRNA-1275 suppresses cell growth, and retards G1/S transition in human nasopharyngeal carcinoma by down-regulation of HOXB5

Sun, Ke; Peng, Tao; Chen, Zhe; Huang, Jing; Zhou, Xuhong

doi:10.1007/s12079-016-0351-9

Cited by 24 publications

(21 citation statements)

References 36 publications

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“…24 It was reported that miR-1275 exhibited its tumour suppressive role on nasopharyngeal carcinoma cells by down-regulating oncogenic HOXB5, whose expression was negatively related to miR-1275. 25 In our study, we found that miR-1275 was lowly expressed in glioma and its up-regulation could suppress glioma cell function including proliferation, migration and invasion, as well as induce apoptosis. Our discovery was consistent with the generally reported conclusion that miR-1275 works as a tumour suppressor.…”

Section: Discussionmentioning

confidence: 62%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

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Section: Discussionmentioning

confidence: 62%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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“…Over the past decades, the emerging role of ncRNAs, including miRNAs, have been reported in the development of EC radioresistance. [28][29][30][31] In this study, we proved that miR-1275, which has been revealed to exert paradoxical functions in different cancer types, 12,[32][33][34] was down-regulated in radioresistant EC cells. Meanwhile, we illustrated that miR-1275 could increase the sensitivity of EC cells to radiotherapy both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 65%

MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

Xie

You-yi

Fei

et al. 2019

J Cellular Molecular Medi

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Acquired radioresistance is one of the main obstacles for the anti‐tumour efficacy of radiotherapy in oesophageal cancer (EC). Recent studies have proposed microRNAs (miRNAs) as important participators in the development of radioresistance in various cancers. Here, we investigated the role of miR‐1275 in acquired radioresistance and epithelial‐mesenchymal transition (EMT) in EC. Firstly, a radioresistant cell line KYSE‐150R was established, with an interesting discovery was observed that miR‐1275 was down‐regulated in KYSE‐150R cells compared to the parental cells. Functionally, miR‐1275 inhibition elevated radioresistance in KYSE‐150 cells via promoting EMT, whereas enforced expression of miR‐1275 increased radiosensitivity in KYSE‐150R cells by inhibiting EMT. Mechanically, we demonstrated that miR‐1275 directly targeted WNT1 and therefore inactivated Wnt/β‐catenin signalling pathway in EC cells. Furthermore, WNT1 depletion countervailed the promoting effect of miR‐1275 suppression on KYSE‐150 cell radioresistance through hampering EMT, whereas WNT1 overexpression rescued miR‐1275 up‐regulation‐impaired EMT to reduce the sensitivity of KYSE‐150R cells to radiation. Collectively, our findings suggested that miR‐1275 suppressed EMT to encourage radiosensitivity in EC cells via targeting WNT1‐activated Wnt/β‐catenin signalling, providing a new therapeutic outlet for overcoming radioresistance of patients with EC.

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“…Liu et al have shown that miR-1275 accelerates the proliferation and migration of head and neck squamous cell carcinoma cells [11]. miR-1275 modulates the proliferation of nasopharyngeal cancer cells by targeting HOXB5 [12]. However, the function of miR-1275 in prostate cancer remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The intercommunication between different cells via exosomal miRNAs is suggested to be an important way of interaction between tumor cells and the tumor microenvironment [9]. Multiple studies have been demonstrated that miR-1275 plays an important role in cancer progression [10][11][12]. Using the whole miRNome analysis, Nam et al have shown that miR-1275 is a potential candidate associated with PCa metastasis [13].…”

Section: Introductionmentioning

confidence: 99%

Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade

Zou¹,

Dai²,

Deng³

et al. 2020

Preprint

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BackgroundCancer-derived exosomes can promote tumor metastasis by delivering miRNAs from cancer cells to recipient cells. miR-1275 is suggested to be an important regulator involved in prostate cancer (PCa) metastasis; however, the association of miR-1275 with the microenvironment of PCa bone metastasis is unclear.Materials and methodsThe level of relative genes and proteins were detected by qPCR and western-blot, cell ability were examined by CCK-8 , the identification of target gene was analyzed by dual-luciferase reporter assay,exosomes were isolated from the PC3-derived conditioned medium by ultracentrifugation.Resultwe found that miR-1275 could be transferred from PCa cells to osteoblasts via exosomes. Exosomal miR-1275 significantly accelerated the proliferation of osteoblasts and the expression levels of osteoblast-specific genes, such as osteocalcin (OCN), type I collagen (COL-1), and osteopontin (OPN). Moreover, exosomal miR-1275 increased the expression of RUNX2, a master modulator of osteoblast differentiation, by down-regulation of SIRT2, which in turn influenced the proliferation and differentiation of osteoblasts.ConclusionsOur findings indicate that exosomal miR-1275 is a promoter of osteoblast activity, which may contribute to PCa bone metastasis.

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MicroRNA-1275 suppresses cell growth, and retards G1/S transition in human nasopharyngeal carcinoma by down-regulation of HOXB5

Cited by 24 publications

References 36 publications

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

MicroRNA‐1275 induces radiosensitization in oesophageal cancer by regulating epithelial‐to‐mesenchymal transition via Wnt/β‐catenin pathway

Prostate Cancer-Secreted Exosomal miR-1275 Modulates Osteoblast Proliferation and Differentiation by Targeting SIRT2/RUNX2 Cascade

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