MicroRNA-128 coordinately targets Polycomb Repressor Complexes in glioma stem cells

Abstract

MiR-128 is an important suppressor of PRC activity, and its absence is an early event in gliomagenesis.

miR-128 downregulation promotes growth and metastasis of bladder cancer cells and involves VEGF-C upregulation

“…Western blot analysis was performed as described previously (13). Protein levels were quantified by Bradford assay (30).…”

“…In addition, miR-128 inhibits tumor growth and angiogenesis by targeting p70S6K1 (10), promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2 (11) and modulates glioma progression by regulating the SNAI1, miR-128 and SP1 axis (12). miR-128 has been demonstrated to target polycomb repressor complexes in glioma stem cells, mediating cancer stem cell maintenance and radioresistance (13). miR-128 has also been demonstrated to serve a role in other types of cancer.…”

MicroRNA-128-3p is a novel oncomiR targeting PHF6 in T-cell acute lymphoblastic leukemia

“…However, miR-128-3p overexpression did not significantly suppress the expression levels of these four genes in T-ALL cell lines (Online Supplementary Figure S4). In addition, we tested other validated miR-128-3p target tumor suppressor genes in other tumor entities including BAX 13 and E2F5 14 (and SUZ12 15 and NF1…”

MicroRNA and extracellular vesicles in glioblastoma: small but powerful

“…We have shown that miR-128 coordinately targets both PRC components PRC1 by targeting B lymphoma Mo-MLV insertion region 1 homolog (BMI1) and PRC2 by targeting suppressor of Zeste 12 Homolog (SUZ12), epigenetic regulators of chromatin status. MiR-128 mediated inhibition of both PRCs blocks their partially redundant function [52] and associated chromatin modifications. Stable overexpression of miR-128 in GSCs changed their molecular landscape by downregulating signaling pathways related to the maintenance of stemness properties.…”