microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1

Dai, Yang; Zhang, Wei Ran; Wang, Yi Min; Liu, Xin Feng; Li, Xin; Xiang, Dongxi; Guo, Hong

doi:10.1007/s11010-016-2656-7

Cited by 39 publications

(30 citation statements)

References 31 publications

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“…Recent research have investigated the functional mechanism of miRNAs in influencing bovine skeletal muscle cell proliferation and differentiation (Dai et al, 2016;Song et al, 2018;Tong, Jiang, Zhang, & Yan, 2017;, but merely a few miRNAs in regulation of muscle cells apoptosis (Wei et al, 2017). In present study, we investigated the expression characteristic of miR-148a-3p…”

Section: Discussionmentioning

confidence: 98%

miR‐148a‐3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6

Yang

Jiang

Song

et al. 2019

Journal Cellular Physiology

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Skeletal muscle development is regulated by a series of regulatory factors, and also including noncoding RNA, especially microRNAs (miRNAs). Recently, miR‐148a has been found to be involved in murine C2C12 differentiation by targeting ROCK1. However, the function of miR‐148a‐3p for the proliferation and apoptosis of bovine muscle cells has not been determined. In this study, we found that miR‐148a‐3p was highly expressed in fetal bovine skeletal muscle and exhibited a decreasing trend in muscle cells during its growth phase. Functional studies indicated that gain of miR‐148a‐3p inhibited the proliferation of bovine muscle cells and promoted apoptosis. Conversely, interference with miR‐148a‐3p inhibitor promoted muscle cell proliferation and inhibited its apoptosis. Mechanistically, KLF6 was confirmed as a new potential target gene of miR‐148a‐3p by TargetScan software prediction and the dual‐luciferase assay verification. Additionally, after a gain or loss of KLF6, the function of KLF6 for muscle cell proliferation and apoptosis was opposite to that of miR‐148a‐3p. Collectively, these findings proposed a novel avenue whereby miR‐148a‐3p impeded bovine myoblast cell proliferation and promoted apoptosis through the posttranscriptional downregulation of KLF6.

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Section: Discussionmentioning

confidence: 98%

miR‐148a‐3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6

Yang

Jiang

Song

et al. 2019

Journal Cellular Physiology

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“…Migliore and colleagues support the idea of post‐transcriptional regulation of MET by showing that also miR‐34b, miR‐34c, and miR‐199a‐5p negatively regulate MET expression and thereby inhibit invasive growth in different cancer cell lines (Migliore et al ., 2008). In bovine skeletal muscle satellite cells, miR‐128‐3p directly target SP1 through this inhibiting cell proliferation (Dai et al ., 2016). As SP1 is involved in TGFβ1‐induced MET expression (Zhang et al ., 2005) in renal epithelial cells, MET might be also regulated by miR‐128‐3p via SP1 in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

TGFβ1 regulates HGF‐induced cell migration and hepatocyte growth factor receptor MET expression via C‐ets‐1 and miR‐128‐3p in basal‐like breast cancer

et al. 2018

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Breast cancer is the most common cancer in women worldwide. The tumor microenvironment contributes to tumor progression by inducing cell dissemination from the primary tumor and metastasis. TGFβ signaling is involved in breast cancer progression and is specifically elevated during metastatic transformation in aggressive breast cancer. In this study, we performed genomewide correlation analysis of TGFBR2 expression in a panel of 51 breast cancer cell lines and identified that MET is coregulated with TGFBR2. This correlation was confirmed at the protein level in breast cancer cell lines and human tumor tissues. Flow cytometric analysis of luminal and basal‐like breast cancer cell lines and examination of 801 tumor specimens from a prospective cohort of breast cancer patients using reverse phase protein arrays revealed that expression of TGFBR2 and MET is increased in basal‐like breast cancer cell lines, as well as in triple‐negative breast cancer tumor tissues, compared to other subtypes. Using real‐time cell analysis technology, we demonstrated that TGFβ1 triggered hepatocyte growth factor (HGF)‐induced and MET‐dependent migration in vitro. Bioinformatic analysis predicted that TGFβ1 induces expression of C‐ets‐1 as a candidate transcription factor regulating MET expression. Indeed, TGFβ1‐induced expression of ETS1 and breast cancer cell migration was blocked by knockdown of ETS1. Further, we identified that MET is a direct target of miR‐128‐3p and that this miRNA is negatively regulated by TGFβ1. Overexpression of miR‐128‐3p reduced MET expression and abrogated HGF‐induced cell migration of invasive breast cancer cells. In conclusion, we have identified that TGFβ1 regulates HGF‐induced and MET‐mediated cell migration, through positive regulation of C‐ets‐1 and negative regulation of miR‐128‐3p expression in basal‐like breast cancer cell lines and in triple‐negative breast cancer tissue.

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“…Gene expression tools are often used to study metabolic pathways in farm animals, particularly of IMF. These works are highly valuable for clean meat research, as they are not aimed for regenerative medicine, but rather focus on meat traits, such as marbling (Lee et al, 2010;Sadkowski et al, 2014;Chen et al, 2015;Hudson et al, 2015;Meadus et al, 2018), myogenesis (Wang et al, 2005(Wang et al, , 2015Lee et al, 2014;Dai et al, 2016), and sensory qualities (Bernard et al, 2007;Hocquette et al, 2007;Bongiorni et al, 2016;Tizioto et al, 2016). Research on correlations between gene expression and meat quality has been recently summarized in several publications.…”

Section: Gene Expressionmentioning

confidence: 99%

“…miRNA, short RNA molecules that inhibit genes with complementary mRNA, play a crucial role in the regulation of muscle tissue development, including myogenesis (Wang et al, 2015;Dai et al, 2016;Zhang et al, 2017;Sadkowski et al, 2018) and adipogenesis (Yan et al, 2013). Several naturally occurring miRNA molecules have been shown to regulate myogenesis and adipogenesis in farm animals, including regulation of PPARG and C/EBPα expression (Romao et al, 2011) and lipid metabolism (Wilfred et al, 2007).…”

Section: Gene Expressionmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

201

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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microRNA-128 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells by repressing Sp1

Cited by 39 publications

References 31 publications

miR‐148a‐3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6

miR‐148a‐3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6

TGFβ1 regulates HGF‐induced cell migration and hepatocyte growth factor receptor MET expression via C‐ets‐1 and miR‐128‐3p in basal‐like breast cancer

Tissue Engineering for Clean Meat Production

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