MicroRNA‐130a inhibits spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells

Li, Cailing; Yang, Bo; Pan, Peng; Ma, Qian; Wu, Yong; Zeng, Zhen; Guo, Xin; Ye, Jing; Gui, Yaoting

doi:10.1002/mrd.23058

Cited by 19 publications

(11 citation statements)

References 38 publications

(57 reference statements)

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“…We established AR, ADIPOQ and TNFα as miR-130a target genes in luciferase reporter assays. While TNFα regulation via miR-130a already has been shown in cervical cancer cells by EGFP reporter assays and Western blotting [38], only indirect hints on a miR-130a regulation of AR existed so far in prostate carcinoma cell lines [37] and mouse sertoli cells [39]. Another putative target gene of miR-130a -PPARγexhibited only a trend towards significance in our experiments for a regulation by miR-130a.…”

Section: Discussioncontrasting

confidence: 59%

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation

et al. 2020

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Objectives: Adipogenesis is the differentiation process generating mature adipocytes from undifferentiated mesenchymal stem cells. The differentiation can be inhibited by androgens, although knowledge about intracellular effectors of this inhibition is scarce. Recently, androgen-regulated microRNAs were detected as interesting candidates in this context. In this study, we analyse the role of miR-130a and miR-301 in the adipogenesis of human SGBS preadipocytes and whether they are prone to androgen regulation. Materials and Methods: microRNA expression during adipogenic differentiation with or without androgen stimulation was measured by qPCR. Putative target genes of miR-130a and miR-301 were identified by target database search and validated in luciferase reporter assays. Results: miR-130a and miR-301 are both significantly downregulated on day 3 and day 5 of adipogenic differentiation in comparison to day 0. Under androgen stimulation, a significant upregulation of miR-130a was detected after 7 days of adipogenesis lasting to day 14, while miR-301 did not change significantly until day 14. Luciferase reporter assays revealed the androgen receptor (AR), adiponectin (ADIPOQ) and tumour necrosis factor alpha (TNFα) as miR-130a target genes. Conclusions: miR-130a is an androgen-regulated microRNA that is downregulated during the early phase of adipogenesis and exerts its functions by regulating AR and ADIPOQ translation. These data may help to identify new signalling pathways associated with the androgen-mediated inhibition of adipogenesis.

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Section: Discussioncontrasting

confidence: 59%

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation

et al. 2020

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“…The DEmiRNAs identified in the soft-shelled turtle belong to 439 families after mapping on the genome, including let-7, miR-10, miR-130, miR-133, miR-138, miR-145, miR-143, miR-202, miR-224, and miR-378. In the majority of cases, the miRNAs and their targets were correlated with animal reproduction [36][37][38][39]. MiR-202-3p could regulate human Sertoli cell proliferation, apoptosis, and synthesis functions by targeting LRP6 and cyclin D1, which belong to the Wnt/β-catenin signalling pathway [40].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

Cen

Wang

et al. 2020

BMC Genomics

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Background: The gonad is the major factor affecting animal reproduction. The regulatory mechanism of the expression of protein-coding genes involved in reproduction still remains to be elucidated. Increasing evidence has shown that ncRNAs play key regulatory roles in gene expression in many life processes. The roles of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in reproduction have been investigated in some species. However, the regulatory patterns of miRNA and lncRNA in the sex biased expression of protein coding genes remains to be elucidated. In this study, we performed an integrated analysis of miRNA, messenger RNA (mRNA), and lncRNA expression profiles to explore their regulatory patterns in the female ovary and male testis of Pelodiscus sinensis. Results: We identified 10,446 mature miRNAs, 20,414 mRNAs and 28,500 lncRNAs in the ovaries and testes, and 633 miRNAs, 11,319 mRNAs, and 10,495 lncRNAs showed differential expression. A total of 2814 target genes were identified for miRNAs. The predicted target genes of these differentially expressed (DE) miRNAs and lncRNAs included abundant genes related to reproductive regulation. Furthermore, we found that 189 DEmiRNAs and 5408 DElncRNAs showed sex-specific expression. Of these, 3 DEmiRNAs and 917 DElncRNAs were testis-specific, and 186 DEmiRNAs and 4491 DElncRNAs were ovary-specific. We further constructed complete endogenous lncRNA-miRNA-mRNA networks using bioinformatics, including 103 DEmiRNAs, 636 DEmRNAs, and 1622 DElncRNAs. The target genes for the differentially expressed miRNAs and lncRNAs included abundant genes involved in gonadal development, including Wt1,

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“…The DEmiRNAs identi ed in the soft-shelled turtle belong to 439 families after mapping on the genome, including let-7, miR-10, miR-130, miR-133, miR-138, miR-145, miR-143, miR-202, miR-224, and miR-378. In the majority of cases, the miRNAs and their targets were correlated with animal reproduction [36][37][38][39]. MiR-202-3p could regulate human Sertoli cell proliferation, apoptosis, and synthesis functions by targeting LRP6 and cyclin D1, which belong to the Wnt/β-catenin signalling pathway [40].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

Cen

Wang

et al. 2020

Preprint

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Abstract Background: The gonad is the major factor affecting animal reproduction. The regulatory mechanism of the expression of protein-coding genes involved in reproduction still remains to be elucidated. Increasing evidence has shown that ncRNAs play key regulatory roles in gene expression in many life processes. The roles of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) in reproduction have been investigated in some species. However, the regulatory patterns of miRNA and lncRNA in the sex biased expression of protein coding genes remains to be elucidated. In this study, we performed an integrated analysis of miRNA, messenger RNA (mRNA), and lncRNA expression profiles to explore their regulatory patterns in the female ovary and male testis of Pelodiscus sinensis.Results: We identified 10 446 mature miRNAs, 20 414 mRNAs and 28 500 lncRNAs in the ovaries and testes, and 633 miRNAs, 11 319 mRNAs, and 10 495 lncRNAs showed differential expression. A total of 2 814 target genes were identified for miRNAs. The predicted target genes of these differentially expressed (DE) miRNAs and lncRNAs included abundant genes related to reproductive regulation. Furthermore, we found that 189 DEmiRNAs and 5 408 DElncRNAs showed sex-specific expression. Of these, 3 DEmiRNAs and 917 DElncRNAs were testis-specific, and 186 DEmiRNAs and 4 491 DElncRNAs were ovary-specific. We further constructed complete endogenous lncRNA-miRNA-mRNA networks using bioinformatics, including 103 DEmiRNAs, 636 DEmRNAs, and 1 622 DElncRNAs. The target genes for the differentially expressed miRNAs and lncRNAs included abundant genes involved in gonadal development, including Wt1, Creb3l2, Gata4, Wnt2, Nr5a1, Hsd17, Igf2r, H2afz, Lin52, Trim71, Zar1, and Jazf1.Conclusions: In animals, miRNA and lncRNA as master regulators regulate reproductive processes by controlling the expression of mRNAs. Considering their importance, the identified miRNAs, lncRNAs, and their targets in P. sinensis might be useful for studying the molecular processes involved in sexual reproduction and genome editing to produce higher quality aquaculture animals. A thorough understanding of ncRNA-based cellular regulatory networks will aid in the improvement of P. sinensis reproductive traits for aquaculture.

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MicroRNA‐130a inhibits spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells

Cited by 19 publications

References 38 publications

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation

MiR-130a in the adipogenesis of human SGBS preadipocytes and its susceptibility to androgen regulation

Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

Genome-wide identification and comparison of differentially expressed profiles of miRNAs and lncRNAs with associated ceRNA networks in the gonads of Chinese soft-shelled turtle, Pelodiscus sinensis

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