MicroRNA‑132‑3p regulates cell proliferation, apoptosis, migration and invasion of liver cancer by targeting Sox4

Abstract

The present study investigated whether microRNA (miR)-132-3p targeted transcription factor SOX-4 (Sox4) for the inhibition of proliferation, migration, invasion and promotion of apoptosis in liver cancer (LC) cells. The expression of miR132-3p and Sox4 mRNA was evaluated by quantitative PCR and protein expression was determined by western blot analysis. Cell proliferation, apoptosis, migration, and invasion were assessed at different time points by the MTT assay, flow cytometry analysis, wound healing assay and Transwell migration assay, respectively. Bioinformatics prediction and luciferase assays were performed to validate and confirm Sox4as a potential target of miR-132p. There was a reduced expression of miR-132-3p in HepG2 and Huh7 cell lines compared with HccLM3 cells. Overexpression of miR-132-3p resulted in significant inhibition of proliferation and induction of apoptosis in LC cells. Moreover, migration and invasion of HepG2 cells were suppressed by over expressing miR-132-3p. However, downregulation of miR-132-3p in Hep-G2 cells promoted cell growth, invasion and migration and inhibited apoptosis. Bioinformatics analysis predicted Sox4 as a potential target of miR-132-3p, which was further confirmed by the luciferase reporter assay. In addition, an inverse association was observed between miR-132-3p and Sox4 expression. miR-132-3p may regulate the proliferation, apoptosis, migration and invasion of HepG2 cells by targeting Sox4.

Circ-sirt1 inhibits growth and invasion of gastric cancer by sponging miR-132-3p/miR-212-3p and upregulating sirt1 expression

“…Overexpression of miR-132-3p resulted in significant inhibition of proliferation and induction of apoptosis in Langerhans cells (LC) [26]. Additionally, miR-132-3p was identified by integrated analysis of miRNAs and DNA methylation to be a tumor suppressor in lung adenocarcinoma [27].…”

SOX4 as biomarker in hepatitis B virus-associated hepatocellular carcinoma

“…Multiple studies have reported the action of SOX4 as an oncogene in solid tumors [7,38,39]. It has been reported that SOX4 is upregulated in various malignancies, including HCC, pancreatic cancer, bladder carcinoma, prostate cancer, breast cancer, colorectal cancer, gastric cancer and melanoma [11,12,14,32,[40][41][42][43][44], raising the potential of this gene as a diagnostic marker. The Mas and Wurmbch liver cancer datasets have reported that SOX4 is highly expressed in hepatitis C virus-associated HCC [26,27], which is consistent with our results.…”

The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells

“…In the present study, DEmiRNAs, such as miR-132, were found to exhibit apoptotic effects and regulate multiple cellular pathways. miR-132 has been shown to be involved in the death of HccLM3 cells, which act as a liver cancer cell model, and the expression of miR-132 progressively increases in HccLM3 cells [ 63 ]. The results of the current study motivate us to speculate that RBP4 overexpression causes cellular and molecular changes in the ovary, where miRNAs may play a central role.…”