microRNA-133b downregulation and inhibition of cell proliferation, migration and invasion by targeting matrix metallopeptidase-9 in renal cell carcinoma

Abstract

microRNA (miRNA)-133b has been revealed to be downregulated in head and neck/oral, bladder, human non-small cell lung, colorectal and esophageal squamous cell cancer. The present study examined the expression of miR-133b in renal cell carcinoma (RCC) cell lines and the effects of miRNA-133b on RCC cell proliferation, migration and invasion. Quantitative polymerase chain reaction was used to detect the expression of miR-133b in RCC cell lines. Following transfection of miR-133b, the expression of miR-133b was examined and a cell viability assay, cell migration assay, cell invasion assay, western blot analysis and luciferase assay were conducted in RCC cell lines. The present study revealed that miRNA‑133b was downregulated and inhibited cell proliferation, migration and invasion in 786-O and A498 cells. In addition, to the best of our knowledge, the present study provided the first evidence that miRNA-133b may directly target matrix metallopeptidase 9 (MMP-9) in RCC. The present study also provided evidence that miRNA-133b suppresses cell proliferation, migration and invasion by targeting MMP-9 in RCC cell lines. These results suggested that miRNA-133b may be used for the development of novel molecular markers and therapeutic approaches to inhibit the metastasis of RCC.

Downregulation of miR‐133b predict progression and poor prognosis in patients with urothelial carcinoma of bladder

“…Downregulation of miR‐133b had also been observed in other cancers, including colorectal cancer, gastric cancer, and renal cell carcinoma 19, 20, 21. Furthermore, we found downregulation of miR‐133b associates with aggressive clinicopathological features of UCB.…”

Clinical verification of a novel urinary microRNA panal: 133b, -342 and -30 as biomarkers for diabetic nephropathy identified by bioinformatics analysis

“…miR-133b was reported as a kidney tumor suppressor [23] and was dysregulated in polycystic kidney disease [24]. Wang groups found that the miR-133b-3p which was downregulated in bone marrow mesenchymal stem cell-derived microvesicles of older rats, remarkably inhibited TGF-b1-mediated EMT in HK2 cells, suggesting that these may play a role in the fibrosis of aging renal tissues [25].…”

miR-133b inhibits glioma cell proliferation and invasion by targeting Sirt1

“…Zhao and colleagues reported that miR-133b was downregulated in osteosarcoma tissues, inhibited proliferation, migration and invasion, and promoted apoptosis of osteosarcoma cells [22]. Moreover, Wu et al showed that miR-133b was downregulated in renal cell carcinoma, and suppressed the cell proliferation, migration and invasion by targeting MMP9 [23]. …”