MicroRNA-139 inhibits hepatocellular carcinoma cell growth through down-regulating karyopherin alpha 2

Zhang, Ying; Wang, Baofeng; Liang, Liang; Deng, Yao; Tian, Tian; Dai, Zhijun; Liu, Dong

doi:10.1186/s13046-019-1175-2

Cited by 43 publications

(35 citation statements)

References 43 publications

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“…WONG CARMEN CHAK-LUI et al demonstrated that miR-139 is downregulated in HCC and played as a tumor suppressing role in inhibiting [12]. Moreover, Zan Ying et al reported that miR-139 overexpression led to decreased c-myc in HCC [32]. Our results also showed that miR-139 expression is negatively related with the expression of E2F, Myc, CDK1 and Cyclin B1 detected by western blot.…”

Section: Discussionsupporting

confidence: 66%

CBX3 Regulated by miR-139 Promoted the Development of HCC by Regulating Cell Cycle Progression

Zhang

Yang

Zha

et al. 2020

Preprint

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Background Hepatocellular carcinoma (HCC), comprises of the major primary liver cancer, is one of the most lethal malignancies in the world [1]. Increasing evidence has demonstrated that chromobox protein homolog 3 (CBX3) functioned as an oncogene in different cancers. However, its expression profiles and biological functions in HCC remain exactly unknown. Methods Data of CBX3 expression in HCC acquired from TCGA and GEO databases were analyzed. The biological functions of CBX3 in HCC were examined by in vitro experiments. Bioinformatics analysis, qRT-PCR and western blot were performed to explpore the mechanism of CBX3 involved in HCC. Results CBX3 mRNA was upregulated in HCC tissues, and overexpression of CBX3 mRNA was negatively correlated with malignancies and poor prognosis in HCC patients. Knocking down of CBX3 induced slower growth, less migration and fewer invasions of the HCC cells in vitro. Moreover, bioinformatics analysis and experimental observation indicated that CBX3 expression was correlated with cell cycle regulation proteins of HCC cells. Finally, Starbase predicted that the miR-139 could directly target CBX3 in HCC; Confirmatory experiments verified that miR-139 overexpression attenuated the HCC cells proliferation and migration, which could be reversed by overexpressing CBX3 concurrently. Conclusion Our results concluded that miR-139/CBX3 axis may involve in the HCC development through regulating cell cycle progression and may be a promising target in the treatment of HCC.

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Section: Discussionsupporting

confidence: 66%

CBX3 Regulated by miR-139 Promoted the Development of HCC by Regulating Cell Cycle Progression

Zhang

Yang

Zha

et al. 2020

Preprint

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“…7G ). Among this network, KPNA2 and TOP2A had been experimentally validated as the targeted genes of hsa-miR-139-5p ( Hua et al, 2015 ; Pei, Yin & Liu, 2018 ; Zan et al, 2019 ). However, there was no report about these two circRNAs acting on hsa-miR-139-5p.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have demonstrated that some miRNA-mRNA pairs of the hub subnetwork participate in the regulation of tumor progression. For instance, miR-139-5p is down-regulated in HCC tissues and overexpression of miR-139-5p inhibits HCC cell growth through suppressing KPNA2 ( Zan et al, 2019 ). It was reported that miR-139-5p inhibits cell proliferation and migration by targeting TOP2A in pancreatic cancer and breast cancer cells ( Hua et al, 2015 ; Pei, Yin & Liu, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis of macrophage infiltration related circRNA-miRNA-mRNA regulatory networks in hepatocellular carcinoma

Chen

Zheng

et al. 2020

PeerJ

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Background Macrophage play a crucial role in regulating tumor progression. This study intended to investigate the circular RNA (circRNA) regulatory network associated with macrophage infiltration in hepatocellular carcinoma (HCC). Methods The immune cell fractions of HCC from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium were calculated by Estimation of the Proportion of Immune and Cancer cells algorithm. The differentially expressed mRNAs (DEmRNAs), microRNAs (DEmiRNAs) and circRNAs (DEcircRNAs) were identified from HCC and adjacent non-tumor cases of TCGA or Gene Expression Omnibus database. The DEmRNAs related to macrophage were selected by weighted gene co-expression network analysis and then utilized to generate the circRNA-miRNA-mRNA network. A hub circRNA regulatory network was established based on the co-expressed DEmiRNAs and DEmRNAs owning contrary correlation with the clinical characteristics, survival and macrophage infiltration level. A gene signature based on the DEmRNAs in hub network was also generated for further evaluation. The circRNA binding bite for miRNA was detected by luciferase assay. Results High macrophage fraction predicted good survival for HCC. A circRNA-miRNA-mRNA network was constructed by 27 macrophage related DEmRNAs, 21 DEmiRNAs, and 15 DEcircRNAs. Among this network, the expression of hsa-miR-139-5p was negatively correlated with CDCA8, KPNA2, PRC1 or TOP2A. Hsa-miR-139-5p low or targeted DEmRNA high expression was associated with low macrophage infiltration, high grade, advanced stage and poor prognosis of HCC. Additionally, the risk score generated by 4-DEmRNA signature could reflect the macrophage infiltration status and function as an independent prognostic factor for HCC. Finally, hsa_circ_0007456 acting on hsa-miR-139-5p related network was viewed as the hub circRNA regulatory network. Taken together, some circRNA regulatory networks may be associated with macrophage infiltration, which provides clues for mechanism study and therapeutic strategies of HCC.

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“…Using microarray analysis, we uncovered that NEAT1 might target miR-139 directly and dual luciferase reporter gene assay validated the findings. It has been uncovered that miR-139 dysregulation was connected with onset and progression of assorted malignancies such as prostate [27], hepatocellular [28], and hepatocellular cancer [29]. Besides, miR-139 was also found to partake in the inhibitory function of monomethyl fumarate which exerted on oxidative stress and inflammatory response in intracerebral hemorrhageinduced rat brain damage [30].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA NEAT1 aggravates epilepsy and seizure-induced neuronal damage by regulating microRNA-139/ROCK1 axis

Hao

Tao

et al. 2020

Preprint

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Background Both long non-coding RNA (lncRNA) NEAT1 and microRNA (miR)-139 are crucial gene regulators in various disorders. This study aims to investigate their role in epilepsy and seizure-induced neuronal damage. Methods In this research, rat model of epilepsy was established by pilocarpine induction. The RNA and protein expression in hippocampal tissues and neurons were determined by RT-qPCR and Western blot analysis, respectively. Microarray analysis was used to predict the relationship between NEAT1 and miR-139 or between miR-139 and ROCK1, and dual luciferase reporter gene assay was performed to verify the interaction. The endogenous expression of related genes was modulated by recombinant plasmids and cell transfection. The cell apoptosis, levels of inflammatory factors and cell proliferation were detected by flow cytometry, ELISA and EdU assay. Results LncRNA NEAT1 and ROCK1 was upregulated, while the miR-139 was downregulated in hippocampal tissues and neurons of epileptic rats. Overexpression of NEAT1 decreased the activity of neurons, increased cell apoptosis, and increased the level of inflammatory factors. NEAT1 negatively targeted miR-139 to upregulate ROCK1. The RhoA/ROCK1 signaling pathway was activated by NEAT1 overexpression and miR-139 downregulation. Conclusion LncRNA NEAT1 suppressed pilocarpine-induced epilepsy by inhibiting apoptosis of hippocampal neurons through miR-139/RhoA/ROCK1 axis, and thereby inhibiting neuronal injury induced by seizure.

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MicroRNA-139 inhibits hepatocellular carcinoma cell growth through down-regulating karyopherin alpha 2

Cited by 43 publications

References 43 publications

CBX3 Regulated by miR-139 Promoted the Development of HCC by Regulating Cell Cycle Progression

CBX3 Regulated by miR-139 Promoted the Development of HCC by Regulating Cell Cycle Progression

Construction and analysis of macrophage infiltration related circRNA-miRNA-mRNA regulatory networks in hepatocellular carcinoma

Long non-coding RNA NEAT1 aggravates epilepsy and seizure-induced neuronal damage by regulating microRNA-139/ROCK1 axis

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