MicroRNA‐145 transcriptionally regulates Semaphorin 3A expression in prostate cancer cells

Barlak, Neslişah; Capik, Ozel; Kilic, Ahsen; Şanli, Fatma; Aytatli, Abdülmelik; Yazıcı, Ayşenur; Karataş, Elanur Aydın; Örtücü, Serkan; Karataş, Ömer Faruk

doi:10.1002/cbin.11554

Cited by 8 publications

(3 citation statements)

References 46 publications

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“…Interestingly, nuclear mature miRNAs can activate rather than repress the transcription of target mRNAs through a new mechanism termed RNA activation (RNAa) [ 6 ]. Briefly, nuclear mature miRNAs act as an endogenous small activating RNA (saRNA) directly interacting with MRE motifs in the promoter region of target mRNAs by base pairing in order to alter histone modifications and recruit RNA polymerase II, finally affecting the transcriptional activity of target mRNAs [ 6 , 7 , 8 ]. However, only a few miRNAs have been identified as controlling the transcription of target mRNAs through an RNAa mechanism.…”

Section: Introductionmentioning

confidence: 99%

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

Wang

Zeng

et al. 2022

Antioxidants

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Phenotypes are the result of the interaction between the gene and the environment, so the response of individuals with different genotypes to an environment is variable. Here, we reported that a mutation in miR-23a influences granulosa cells (GCs) response to oxidative stress, a common mechanism of environmental factors affecting female reproduction. We showed that nuclear miR-23a is a pro-apoptotic miRNA in porcine GCs through the activation of the transcription and function of NORHA, a long non-coding RNA (lncRNA) induces GC apoptosis and responses to oxidative stress. Mechanistically, miR-23a acts as an endogenous small activating RNA (saRNA) to alter histone modifications of the NORHA promoter through the direct binding to its core promoter. A C > T mutation was identified at −398 nt of the miR-23a core promoter, which created a novel binding site for the transcription factor SMAD4 and recruited the transcription repressor SMAD4 to inhibit miR-23a transcription and function in GCs. Notably, g.−398C > T mutation in the miR-23a promoter reduced GCs response to oxidative stress. In addition, g.−398C > T mutation was significantly associated with sow fertility traits. In short, our findings preliminarily revealed the genetic basis of individual differences in the response to oxidative stress from the perspective of a single mutation and identified miR-23a as a candidate gene for the environmental adaptation to oxidative stress.

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Section: Introductionmentioning

confidence: 99%

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

Wang

Zeng

et al. 2022

Antioxidants

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“…However, further research is required to determine how well these findings reflect endogenous miRNA functions in vivo. Emerging evidence regarding miRNAs has demonstrated the significance of the regulatory mechanisms of nuclear miRNA expression in cancer (93,94). The critical role of nuclear miRNAs in tumor initiation, growth and metastasis is discussed below.…”

Section: Transcriptional Regulation By Nuclear Mirna-mediated Epigene...mentioning

confidence: 99%

Transcriptional regulation of nuclear miRNAs in tumorigenesis (Review)

et al. 2022

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MicroRNAs (miRNAs/miRs) are a type of endogenous non-coding small RNA that regulates gene expression. miRNAs regulate gene expression at the post-transcriptional level by targeting the 3'-untranslated region (3'UTR) of cytoplasmic messenger RNAs (mRNAs). Recent research has confirmed the presence of mature miRNAs in the nucleus, which bind nascent RNA transcripts, gene promoter or enhancer regions, and regulate gene expression via epigenetic pathways. Some miRNAs have been shown to function as oncogenes or tumor suppressor genes by modulating molecular pathways involved in human cancers. Notably, a novel molecular mechanism underlying the dysregulation of miRNA expression in cancer has recently been discovered, indicating that miRNAs may be involved in tumorigenesis via a nuclear function that influences gene transcription and epigenetic states, elucidating their potential therapeutic implications. The present review article discusses the import of nuclear miRNAs, nucleus-cytoplasm transport mechanisms and the nuclear functions of miRNAs in cancer. In addition, some software tools for predicting miRNA binding sites are also discussed. Nuclear miRNAs supplement miRNA regulatory networks in cancer as a non-canonical aspect of miRNA action. Further research into this aspect may be critical for understanding the role of nuclear miRNAs in the development of human cancers. Contents 1. Introduction 2. The process of miRNAs entering the nuclei 3. Argonaute protein family and its structural characteristics 4. Identification and prediction of nuclear miRNAs 5. Transcriptional regulation by nuclear miRNA-mediated epigenetic modification 6. Role of nuclear miRNAs in the transcriptional regulation of tumor cells 7. conclusions and future perspectives

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“…The progression of prostate cancer involves several molecular mechanisms, underlying a complex regulation of autophagy [113][114][115][116][117], micro-RNA [118][119][120][121][122], p53 [123][124][125][126], Bcl-2 [127,128] family and interleukins [31,32,129,130] able to also impinge on the above-mentioned metabolisms.…”

Section: One-carbon and Serine Metabolism Impact On Prostate Cancer Progressionmentioning

confidence: 99%

Serine and one-carbon metabolisms bring new therapeutic venues in prostate cancer

et al. 2021

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Serine and one-carbon unit metabolisms are essential biochemical pathways implicated in fundamental cellular functions such as proliferation, biosynthesis of important anabolic precursors and in general for the availability of methyl groups. These two distinct but interacting pathways are now becoming crucial in cancer, the de novo cytosolic serine pathway and the mitochondrial one-carbon metabolism. Apart from their role in physiological conditions, such as epithelial proliferation, the serine metabolism alterations are associated to several highly neoplastic proliferative pathologies. Accordingly, prostate cancer shows a deep rearrangement of its metabolism, driven by the dependency from the androgenic stimulus. Several new experimental evidence describes the role of a few of the enzymes involved in the serine metabolism in prostate cancer pathogenesis. The aim of this study is to analyze gene and protein expression data publicly available from large cancer specimens dataset, in order to further dissect the potential role of the abovementioned metabolism in the complex reshaping of the anabolic environment in this kind of neoplasm. The data suggest a potential role as biomarkers as well as in cancer therapy for the genes (and enzymes) belonging to the one-carbon metabolism in the context of prostatic cancer.

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MicroRNA‐145 transcriptionally regulates Semaphorin 3A expression in prostate cancer cells

Cited by 8 publications

References 46 publications

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

A Mutation in Endogenous saRNA miR-23a Influences Granulosa Cells Response to Oxidative Stress

Transcriptional regulation of nuclear miRNAs in tumorigenesis (Review)

Serine and one-carbon metabolisms bring new therapeutic venues in prostate cancer

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