MicroRNA-146a Enhances Helicobacter pylori Induced Cell Apoptosis in Human Gastric Cancer Epithelial Cells

Wu, Kai; Liu, Yang; Liu, Cong; Zhu, Chao-Hui; Wang, Xin; Yao, Yi; Jia, Yujie

doi:10.7314/apjcp.2014.15.14.5583

Cited by 19 publications

(14 citation statements)

References 15 publications

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“…An Annexin V-Fluos staining kit (Roche-Boehringer) was used to detect early stages of apoptosis according with previous report (9). The cells were washed with phosphate-buffered saline (PBS) and stained according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Wu et al (9) showed that miR-146a enhances Helicobacter pylori-induced cell apoptosis in human gastric cancer epithelial cells by targeting COX-2. Hao et al (22) demonstrated that enforced expression of miR-101 inhibits prostate cancer cell growth by modulating the COX-2 pathway.…”

Section: A B C Dmentioning

confidence: 99%

“…miRNAs are a class of small noncoding RNAs that are implicated in numerous physiological and pathological responses as post-transcriptional repressors of gene expression. Mature miRNAs can specifically bind to the 3'-untranslated regions (3'-UTRs) of target cellular mRNA, in turn triggering mRNA degradation or inhibition of translation (9). A previous study has reported that miRNA-26a (miR-26a) had decreased expression in prostate cancer (10).…”

Section: Introductionmentioning

confidence: 99%

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Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2

2016

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Abstract. ) is expressed at lower levels in prostate cancer cells compared with normal prostate cells. However, the regulatory mechanism of miR-26a in tumorigenesis and metastasis is not clear. In the present study, the expression profile of cellular miR-26a was analyzed by reverse transcription-quantitative polymerase chain reaction. The potential target of miR-26a was identified by luciferase assay and western blotting. Examination of miR-26a function was performed by transfection with miR-26a mimics and inhibitor. It was found that miR-26a expression was decreased in prostate cancer tissues and cell lines, with androgen-independent prostate cancer (AIPC) showing lower miR-26a expression compared with androgen-dependent prostate cancer (ADPC). Overexpression of miR-26a by transfecting miR-26a mimics could significantly enhance apoptosis, and this upregulation of apoptosis was triggered by cytochrome c oxidase subunit II inhibition. Furthermore, it was found that lower miR-26a density resulted in an evidently poor prognosis. Understanding the important roles of miR-26a in regulating cell apoptosis in human prostate cancer cells may aid the exploration of AIPC transformation mechanisms and contribute to the development of miRNA-based therapy in the future.

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Section: Methodsmentioning

confidence: 99%

Section: A B C Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2

2016

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“…development, cell proliferation, cell cycle, apoptosis, migration, and differentiation (Yin et al, 2012;Chen et al, 2013;Montalban et al, 2014;Wu et al, 2014a;2014b;Zha et al, 2014;Zhong et al, 2014). They have also gained importance as they carry information about the pathophysiological nature of a disease, and thus can serve as ideal predictive biomarkers and therapeutic targets.…”

Section: Introductionmentioning

confidence: 99%

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Tang²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…According to Wu et al (2014), miR-146a density was correlated with GC. Specifically, miR-146a density was positively associated with apoptosis rates in H. pylori-positive gastric tissues, which might provide a link to the gastric carcinogenesis process.…”

Section: Discussionmentioning

confidence: 99%

Association between five common polymorphisms in microRNA genes and the risk of gastric cancer: a meta-analysis

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Gastric cancer (GC) is a prevalent disease with a high mortality rate, especially in developing countries. Accumulating evidence suggests that single nucleotide polymorphisms in microRNA (miRNA) genes might influence the susceptibility to GC; such sequence variation might contribute to the development of disease by altering crucial cellular pathways. In this study, we assessed the correlation between the miR-146a G>C, miR-196a-2 C>T, miR-499 T>C, miRNA27a A>G, and miRNA-149 T>C polymorphisms and the susceptibility to GC. A comprehensive literature search for relevant studies published prior to August 2014 was conducted using PubMed/Medline, Embase, Web of Science, the Cochrane Library, and CNKI databases along with Google Scholar. Meta-analysis was performed using odds ratios (ORs) and 95% confidence intervals (CIs) as effect measures, incorporating 19 studies encompassing 8285 patients and 10,716 controls. Allelic, dominant, recessive, homozygous, and heterozygous genetic models were examined. Pooled results showed that none of the five polymorphisms studied were statistically related to GC. Stratified analyses by ethnicity and source of controls were conducted for miR146a G>C and miR-196a-2 C>T. Subgroup analysis suggested that the miR-146a G allele might increase the risk of GC in hospital-based case-control (HCC) but not in population-based case-control studies (HCC: recessive model: OR = 1.23, 95%CI = 1.10-1.37, P < 0.001; heterozygous model: OR = 1.19, 95%CI = 1.06-1.34, P = 0.004). Overall, this meta-analysis failed to detect an association between five common miR-146a gene polymorphisms and GC susceptibility. However, this does not necessarily completely rule out a correlation between miRNA polymorphisms and GC susceptibility.

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MicroRNA-146a Enhances Helicobacter pylori Induced Cell Apoptosis in Human Gastric Cancer Epithelial Cells

Cited by 19 publications

References 15 publications

Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2

Downregulated microRNA-26a modulates prostate cancer cell proliferation and apoptosis by targeting COX-2

Association of miR-193b Down-regulation and miR-196a up-Regulation with Clinicopathological Features and Prognosis in Gastric Cancer

Association between five common polymorphisms in microRNA genes and the risk of gastric cancer: a meta-analysis

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