MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

Bai, Zhenghai; Sun, Jiangli; Wang, Xiaobo; Wang, Hai; Pei, Honghong; Zhang, Zhengliang

doi:10.3892/or.2015.4051

Cited by 41 publications

(31 citation statements)

References 27 publications

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“…MiR‐153 is proposed as a newfangled tumor‐correlative miRNA, which has been discovered to be dramatically deregulated in tumors (Bai et al, ; Chen et al, ). A research by Liang et al () promulgated that hypoxia management could engender the enhancement of miR‐153, thereby affecting breast cancer angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

Lou

Zhang

et al. 2019

Phytotherapy Research

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Ganoderic Acid A (GAA) is often applied for healing cardiovascular and cerebrovascular ailments, but the influences in cerebral ischemia injury are still hazy. The research delved into the functions of GAA in hypoxia‐triggered impairment in PC12 cells. PC12 cells received hypoxia management for 12 hr, and subsequently, cell viability, migration, apoptosis, and correlative protein levels were assessed. After preprocessing with GAA, above cell behaviors were monitored again. The vector of microRNA (miR)‐153 inhibitor was utilized for PC12 cell transfection to further explore the functions of miR‐153 in hypoxia‐impaired cells. Pathways of phosphatidylinositol 3‐kinase (PI3K)/protein kinase B (AKT) and mammalian target of rapamycin (mTOR) were investigated via executing western blot for uncovering the latent mechanism. Results revealed that hypoxia disposition triggered PC12 cells impairment via restraining cell viability and migration and accelerating apoptosis. However, GAA visibly mollified hypoxia‐provoked impairment in PC12 cells. Interestingly, the enhancement of miR‐153 triggered by GAA was observed in hypoxia‐impaired PC12 cells. After miR‐153 inhibitor transfection, the protective functions of GAA in hypoxia‐impaired PC12 cells were dramatically inversed. Furthermore, GAA caused PI3K/AKT and mTOR activations via enhancement of miR‐153 in hypoxia‐impaired PC12 cells. The findings evinced that GAA exhibited the protective functions in PC12 cells against hypoxia‐evoked impairment through activating PI3K/AKT and mTOR via elevating miR‐153.

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Section: Discussionmentioning

confidence: 99%

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

Lou

Zhang

et al. 2019

Phytotherapy Research

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“…In order to evaluate the downstream target genes of miR-30a which are able to inhibit tumor development in breast cancer tissue, the target gene of miR-30a was screened for using the target gene prediction software TargetScans. Among the numerous potential target genes, due to its function in the development of human cancer (26)(27)(28)(29), Snail was selected as the target gene of interest. Snail is a regulatory factor in the process of EMT in tumor cells (30).…”

Section: Discussionmentioning

confidence: 99%

miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail

2018

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The present study aims to investigate the effect of miR-30a on the proliferative and invasive abilities of breast cancer cells, and to observe the role of miR-30a in the pathogenesis of breast cancer. With the increase of pathological grade and malignant degree of breast cancer cells, the miR-30a expression level gradually decreased (P<0.01). Transfection with miR-30a mimic significantly inhibited the proliferative and invasive ability of SK-BR-3 cells (P<0.01), while transfection with anti-miR-30a significantly improved the proliferative and invasive ability of these cells (P<0.01). It was revealed using bioinformatic methods that Snail was the functional target gene of miR-30a, and this was verified by the results of a luciferase reporter gene assay. The results of analysis of Snail expression in breast cancer tissues and breast cancer cells revealed that with the increase in pathological grade and malignant degree of breast cancer cells, Snail expression levels gradually increased (P<0.01). Western blotting revealed that miR-30a significantly inhibited Snail expression in SK-BR-3 cells, upregulated the expression of EMT-associated E-cadherin, and downregulated the expression of EMT-associated N-cadherin and Vimentin. MiR-30a was able to affect the proliferation and invasion of breast cancer cells by regulating Snail expression, and therefore has a regulatory effect on the occurrence and development of breast cancer.

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“…Certain miRNAs have been implicated in cellular processes involving proliferation, invasiveness, apoptosis, and chemoresistance (10)(11)(12)(13). Moreover, current evidence has demonstrated that miRNAs are critically involved in regulating drug resistance-mediated epithelial to mesenchymal transition (EMT) (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-200b and -301 are associated with gemcitabine response as biomarkers in pancreatic carcinoma cells

et al. 2019

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Chemotherapy resistance (congenital or acquired) is one of the principal challenges for the treatment of pancreatic carcinoma. Recent evidence has demonstrated that epithelial to mesenchymal transition (EMT) is associated with chemoresistance in pancreatic carcinoma cells. However, the molecular mechanism underlying the development of chemoresistance remains unknown, and limited therapeutic options are available. Therefore, to anticipate individual chemosensitivity or acquired chemoresistance for patients with pancreatic carcinoma, predictive biomarkers are urgently required. Extensive evidence suggests that microRNAs (miRNAs) serve a crucial role in regulating EMT. The aim of this study was to examine the potential role of miRNA (miR)-200b and miR-301 in predicting the chemo-responses to treatment for pancreatic carcinoma. The present results demonstrate that miR-200b expression predicted chemo-sensitivity and may have potential as a biomarker. In six different pancreatic carcinoma cell lines (Capan-1, Capan-2, Panc-1, MIAPaCa-2, BxPC-3 and PL45 cells), the expression of miR-200b correlated positively with chemosensitivity. Moreover, the enhanced expression of miR-200b increased chemosensitivity and induced mesenchymal to epithelial transition. Conversely, miR-301 modulated gemcitabine resistance and induced EMT through the downregulation of cadherin 1 expression. In addition, gemcitabine-resistant cells (Capan-2 and Panc-1) exhibited upregulated miR-301 expression and downregulated gemcitabine-induced apoptosis. In summary, these two miRNAs may serve roles as biomarkers in pancreatic carcinoma, miR-200b expression may predict chemosensitivity, and elevated miR-301 expression may have potential applications in the prediction of acquired gemcitabine resistance.

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MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

Cited by 41 publications

References 27 publications

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

miR‑30a regulates the proliferation and invasion of breast cancer cells by targeting Snail

MicroRNA-200b and -301 are associated with gemcitabine response as biomarkers in pancreatic carcinoma cells

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