MicroRNA-155 mediates downregulation of the high-affinity receptor for IgE through Toll-like receptor signaling

Leib, Nicole; Herrmann, Nadine; Koch, Susanne; Stroisch, T. J.; Schnautz, Sylvia; Wilms, Helene; Bieber, Thomas

doi:10.1016/j.jaci.2017.08.014

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…Even though the in vitro IDEC and LC are capable to mature after different stimuli in general, 1,25(OH) 2 -D 3 alone did not induce maturation, as we show by expression analyses of the maturation marker CD83 and costimulatory molecules. Thus, in contrast to the regulation of FcεRI by TLR2 ligands we showed previously (16,17), 1,25(OH) 2 -D 3 can regulate FcεRI expression without induction of maturation. In line, we show that 1,25(OH) 2 -D 3 directly regulates FcεRI expression at the transcriptional level of its a-chain in epidermal DC but independently of the microRNA-155-PU.1-axis.…”

Section: Discussioncontrasting

confidence: 64%

“…3D), indicating that 1,25(OH) 2 -D 3 -mediated reduction of FcεRI occurs at the transcriptional level of the receptor's a-chain. FcεRI is regulated by TLR2 ligands via the upregulation of microRNA-155 and a subsequent downregulation of PU.1, being a main TF for FCER1A in in vitro LC (16,17). However, 1,25(OH) 2 -D 3 treatment of IDEC rather downregulated microRNA-155 and upregulated PU.1 (Fig.…”

Section: 25(oh) 2 -D 3 Directly Reduces Fc«ri At the Transcriptional ...mentioning

confidence: 92%

“…FcεRI on DC is composed of one IgE-binding a-chain and two g-chains, the latter being responsible for surface expression and signaling (15). We have previously shown that in LC, TLR2 ligation induces a strong reduction of FcεRI at the transcriptional level of its a-chain, which is mediated by microRNA-155 upregulation and subsequent downregulation of transcription factor (TF) PU.1 (16,17).…”

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confidence: 99%

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Vitamin D3–Induced Promotor Dissociation of PU.1 and YY1 Results in FcεRI Reduction on Dendritic Cells in Atopic Dermatitis

Herrmann

Nümm

Iwamoto

et al. 2021

The Journal of Immunology

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Atopic dermatitis (AD) is a severe inflammatory skin disease. Langerhans cells and inflammatory dendritic epidermal cells (IDEC) are located in the epidermis of AD patients and contribute to the inflammatory processes. Both express robustly the high-affinity receptor for IgE, Fc«RI, and thereby sense allergens. A beneficial role of vitamin D 3 in AD is discussed to be important especially in patients with allergic sensitization. We hypothesized that vitamin D 3 impacts Fc«RI expression and addressed this in human ex vivo skin, in vitro Langerhans cells, and IDEC models generated from primary human precursor cells. We show in this article that biologically active vitamin D 3 [1,25(OH) 2 -D 3 ] significantly downregulated Fc«RI at the protein and mRNA levels of the receptor's a-chain, analyzed by flow cytometry and quantitative RT-PCR. We also describe the expression of a functional vitamin D receptor in IDEC. 1,25(OH) 2 -D 3 -mediated Fc«RI reduction was direct and resulted in impaired activation of IDEC upon Fc«RI engagement as monitored by CD83 expression. Fc«RI regulation by 1,25(OH) 2 -D 3 was independent of maturation and expression levels of microRNA-155 and PU.1 (as upstream regulatory axis of Fc«RI) and transcription factors Elf-1 and YY1. However, 1,25(OH) 2 -D 3 induced dissociation of PU.1 and YY1 from the FCER1A promotor, evaluated by chromatin immunoprecipitation. We show that vitamin D 3 directly reduces Fc«RI expression on dendritic cells by inhibiting transcription factor binding to its promotor and subsequently impairs IgE-mediated signaling. Thus, vitamin D 3 as an individualized therapeutic supplement for those AD patients with allergic sensitization interferes with IgE-mediated inflammatory processes in AD patients.

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Section: Discussioncontrasting

confidence: 64%

Section: 25(oh) 2 -D 3 Directly Reduces Fc«ri At the Transcriptional ...mentioning

confidence: 92%

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confidence: 99%

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Vitamin D3–Induced Promotor Dissociation of PU.1 and YY1 Results in FcεRI Reduction on Dendritic Cells in Atopic Dermatitis

Herrmann

Nümm

Iwamoto

et al. 2021

The Journal of Immunology

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“…miR‐155 is needed for antibody production, B‐cell maturation, differentiation, and immunoglobulin class switching 11 . miR‐155 has also been reported to downregulate FceRI expression and FcεRI‐mediated mast cell degranulation, 12,13 and its expression is increased in human skin‐derived mast cells following FcεRI crosslinking with antigen 14 . CSU patients have not yet been assessed for miR‐155, but patients with atopic dermatitis exhibit increased expression in mast cells at lesional skin sites 15 .…”

Section: Introductionmentioning

confidence: 99%

In chronic spontaneous urticaria, IgE and C‐reactive protein are linked to distinct microRNAs and interleukin‐31

Karstarli Bakay,

Demir,

Cicek

et al. 2023

Clinical & Translational All

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BackgroundChronic spontaneous urticaria (CSU) is a common and disabling disease. Assessments of IgE and C‐reactive protein (CRP) are recommended in the diagnostic work‐up, but the role and clinical relevance of these biomarkers are not well characterized. Moreover, it remains unknown if elevated levels of IgE or CRP are linked to CSU microRNA (miRNA) signatures or interleukin 31 (IL‐31).MethodsWe measured IgE and CRP serum levels in 47 CSU patients (and 45 healthy controls) and determined CSU disease activity using the urticaria activity score (UAS7). Expression levels of miR‐155 and miR‐221 were assessed by RT‐PCR, and IL‐31 levels were determined by ELISA.ResultsTotal IgE and CRP levels were independently increased in CSU patients. IgE and CRP levels were highest and lowest in patients with high and mild disease activity. IgE levels correlated with miR‐155 levels, whereas CRP levels correlated with miR‐221 levels. miR‐155 and miR‐221 were significantly overexpressed in CSU patients. ROC analyses linked miRNA‐155 and CSU with a sensitivity of 79% and specificity of 87%, and miRNA‐221 and CSU with a sensitivity of 75% and specificity of 91%. High CRP and miR‐221 expression levels were linked to elevated levels of IgG anti‐TPO and IL‐31.ConclusionIgE and CRP are useful biomarkers for disease activity in CSU, with distinct miRNA profiles. High CRP and miR‐221 levels may point to autoimmune CSU and a role for IL‐31.

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MicroRNAs in type 2 immunity

2018

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MicroRNA-155 mediates downregulation of the high-affinity receptor for IgE through Toll-like receptor signaling

Cited by 4 publications

References 9 publications

Vitamin D3–Induced Promotor Dissociation of PU.1 and YY1 Results in FcεRI Reduction on Dendritic Cells in Atopic Dermatitis

Vitamin D3–Induced Promotor Dissociation of PU.1 and YY1 Results in FcεRI Reduction on Dendritic Cells in Atopic Dermatitis

In chronic spontaneous urticaria, IgE and C‐reactive protein are linked to distinct microRNAs and interleukin‐31

MicroRNAs in type 2 immunity

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