MicroRNA-15b-5p Predicts Locoregional Relapse in Head and Neck Carcinoma Patients Treated With Intensity-modulated Radiotherapy

Ahmad, Parwez; Šána, Jiří; Slavík, M; Gurín, Dominik; Radová, Lenka; Gabło, Natalia; Kazda, Tomáš; Smilek, Pavel; Hořáková, Zuzana; Gál, Břetislav; Hermanová, Markéta; Šlampa, Pavel; Slabý, Ondřej

doi:10.21873/cgp.20119

Cited by 24 publications

(26 citation statements)

References 29 publications

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“…Numerous studies have reported that miRNAs perform important functions in the development of liver cancer (17–21). Previous studies have demonstrated abnormal levels of miR-221 and miR-15b in liver cancer tissues, which affect the progression of liver cancer and exhibit oncogenic characteristics (9,22–25). Although the mechanisms of action of these miRNAs in tumors are unclear, their importance is evident; miR-221 and miR-15b may be breakthrough targets for tumor prevention, diagnosis and treatment.…”

Section: Discussionmentioning

confidence: 99%

miR‑221‑3p and miR‑15b‑5p promote cell proliferation and invasion by targeting Axin2 in liver cancer

Dong

Zhang²,

Zhao

et al. 2019

Oncol Lett

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Globally, liver cancer has the third highest mortality rate among all types of cancer due to the invasive and metastatic capacities of liver tumor cells. MicroRNA (miR) is a class of non-coding RNAs that participate in the development of liver cancer. The aim of the present study was to explore the molecular mechanisms by which miR-221-3p and miR-15b-5p promote the proliferation and invasion of liver cancer cells through targeting axis formation inhibitor 2 (Axin2) and to identify suitable targets for the treatment of liver cancer. The expression levels of miR-221-3p and miR-15b-5p were determined in liver cancer tissues and cells by quantitative PCR, and the association between miR-221-3p, miR-15b-5p and Axin2 expression in liver cancer cells was analyzed using cell transfection. The results demonstrated that miR-221-3p and miR-15b-5p levels were upregulated in liver cancer tissues and cell lines, and results from predictive bioinformatic analysis and identification revealed that Axin2 was the common target gene of miR-221-3p and miR-15b-5p. miR-221-3p and miR-15b-5p may be used as prognostic indicators for liver cancer. The miR-221-3p/miR-15b-5p-Axin2 axis may serve as a therapeutic target in patients with liver cancer.

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Section: Discussionmentioning

confidence: 99%

miR‑221‑3p and miR‑15b‑5p promote cell proliferation and invasion by targeting Axin2 in liver cancer

Dong

Zhang²,

Zhao

et al. 2019

Oncol Lett

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“…Levels of miRNAs in the saliva samples might be used for detection of oral SCC both at the time of cancer diagnosis and after resection of the primary tumor [21]. In a retrospective study, Ahmad et al have shown that miR-15b-5p is differentially expressed between patients with short and long time of locoregional control in a way that patients with higher levels of this miRNA had a remarkably longer locoregional relapse-free survival [68]. Further prospective studies are needed to verify whether expression level of this miRNAs might be employed for individualized treatment decisions.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA profile in the squamous cell carcinoma: prognostic and diagnostic roles

Ghafouri‐Fard

Gholipour

Taheri

et al. 2020

Heliyon

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Head and neck squamous cell carcinomas (HNSCCs) are human malignancies associated with both genetic and environmental factors. MicroRNAs (miRNAs) as a group of small non-coding RNAs have prominent roles in the development of this kind of cancer. Expressions of several miRNAs have been demonstrated to be increased in HNSCC samples vs. non-malignant tissues. In silico prediction tools and functional analyses have confirmed the function of some miRNAs in the modulation of cancer-associated targets, thus indicating these miRNAs as onco-miRs. Moreover, numerous miRNAs have been down-regulated in HNSCC samples. Their targets mostly enhance cell proliferation or inhibit apoptosis. miRNAs signature has practical implications in the diagnosis, staging, and management of HNSC. Most notably, numerous miRNAs have been shown to alter response of tumor cells to anticancer drugs such as cisplatin and doxorubicin. Circulating levels of these small transcripts have been suggested as promising biomarkers for diagnosis of HNSCC. In the present manuscript, we sum up the available literature regarding the miRNAs signature in HNSCC and their role as diagnostic/prognostic biomarkers.

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“…luo et al (42) demonstrated that mir-15b-5p reduced proliferation, but induced apoptosis and cytotoxic activities of Pc12 cells. Moreover, ahmad et al (43) reported that mir-15b-5p could be a potential biomarker for the treatment of squamous cell carcinoma. mir-15p-5b expression also exhibits a downward trend in osteosarcoma (44), colon cancer (45) and squamous cell carcinoma (46).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑15b‑5p exerts its tumor repressive role via targeting GDI2: A novel insight into the pathogenesis of thyroid carcinoma

Zou

Qian²,

et al. 2020

Mol Med Rep

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Thyroid carcinoma (THca) is a malignant tumor of the endocrine system. Previous studies have revealed the vital roles of micrornas (mirnas/mirs) in THca procession. The present study aimed to explore the effects of mir-15b-5p on the progression of THca and its targeting mechanism. The data of THCA and healthy samples were firstly collected from starbase2.0 and used to analyze the relationship of mir-15b-5p with THca. dual-luciferase assay was performed to detect the direct interaction between mir-15b-5p and the predicted target gene GdP dissociation inhibitor 2 (Gdi2). The effects of mir-15b-5p and Gdi2 on the overall survival of patients with THca were analyzed using Kaplan-Meier analysis with log rank test. cell counting Kit-8 and Transwell assays were conducted to assess the impacts of mir-15b-5p and Gdi2 on the proliferation and invasion of THca cells. reverse transcription-quantitative Pcr and western blot analyses were performed to analyze the expression levels of the related mirnas and proteins, respectively. mir-15b-5p was found to be downregulated both in THca tissues and cells, and the low expression of mir-15b-5p was associated with the short overall survival time of patients. Moreover, the upregulation or downregulation of mir-15b-5p could inhibit or enhance the proliferation and invasion of THca cells, respectively. mir-15b-5p reduced the protein expression levels of matrix metalloproteinase (MMP)2 and MMP9, which were related to cell invasion. Furthermore, Gdi2, which was enhanced in THca and related to the poor prognosis of patients with THCA, was identified as the target gene of miR-15b-5p and negatively regulated by mir-15b-5p. additional experiments demonstrated that GDI2 overexpression could significantly reduce the antitumor effect of mir-15b-5p and its inhibitory action on the expression levels of MMP2 and MMP9. Thus, the results indicated a potential tumor suppressive role of mir-15b-5p in THca, which was mainly exerted by targeting GDI2 and modulating MMP2 and MMP9. These findings will increase the understanding on the pathogenesis of THca and provide novel candidates for THca therapy.

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MicroRNA-15b-5p Predicts Locoregional Relapse in Head and Neck Carcinoma Patients Treated With Intensity-modulated Radiotherapy

Cited by 24 publications

References 29 publications

miR‑221‑3p and miR‑15b‑5p promote cell proliferation and invasion by targeting Axin2 in liver cancer

miR‑221‑3p and miR‑15b‑5p promote cell proliferation and invasion by targeting Axin2 in liver cancer

MicroRNA profile in the squamous cell carcinoma: prognostic and diagnostic roles

MicroRNA‑15b‑5p exerts its tumor repressive role via targeting GDI2: A novel insight into the pathogenesis of thyroid carcinoma

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