MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers

Luthra, Rajyalakshmi; Singh, Rajesh; Luthra, Madan G.; Li, Y. X.; Hannah, Christina; Romans, Angela; Barkoh, Bedia A.; Chen, S. S.; Ensor, Joe; Maru, Dipen M.; Broaddus, Russell R.; Rashid, Asif; Albarracin, Constance T.

doi:10.1038/onc.2008.256

Cited by 240 publications

(215 citation statements)

References 28 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…Ryan et al (39) suggested that variations in miRNAs may be related to the risk of cancer and reported that the rs11614913 polymorphism located in the hsa-miR-196a2 3' mature sequence affects the maturation and may affect target mRNA. Cell culture experiments also indicated that high hsa-miR-196a levels could suppress the activities of various cancer-related genes, such as ANXA1 (Annexin A1), suppression of which is well documented in various cancer types (40,41). Li et al (23) conducted an analysis of rs11614913 genotypes and the expression of mature miR-196a.…”

Section: Discussionmentioning

confidence: 99%

miR-196a2 polymorphisms and susceptibility to cancer: A meta-analysis involving 24,697 subjects

Wang

Xie

Cui

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. An increasing number of studies have shown that the hsa-miR-196a2 rs11614913 polymorphism occurs in different types of cancer, but the results are generally controversial and inadequate, mainly due to limited statistical power. To resolve this issue, the present meta-analysis was carried out. Databases, including PubMed and Embase, were searched using: (miR-196a2 . Crude odds ratios (ORs) with 95% confidence intervals (CIs) were summarized in forest plots and detailed in tables. A total of 20 studies, including 11,004 cases and 13,693 controls, were included in the meta-analysis. The hsa-miR-196a2 rs11614913 polymorphism was significantly associated with an increased cancer risk in all genetic models (CC vs. TT: OR=1.280, 95% CI 1.131-1.449, P<0.001; CT vs. TT: OR=1.187, 95% CI 1.079-1.306, P<0.001; CC/CT vs. TT: OR=1.216, 95% CI 1.104-1.341, P<0.001; and CC vs. CT/TT: OR=1.115, 95% CI 1.025-1.213, P=0.011). In conclusion, this meta-analysis provides compelling evidence that the hsa-miR196a2 rs11614913 polymorphism plays a crucial role in the development of cancer. Screening of patients for the hsa-miR196a2 rs11614913 polymorphism can prove clinically useful for the prediction and prevention of cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

miR-196a2 polymorphisms and susceptibility to cancer: A meta-analysis involving 24,697 subjects

Wang

Xie

Cui

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…For mRNA expression profile of ISCU and COX10, real-time PCR analysis was performed using TaqMan gene expression assays as described previously (Luthra et al, 2008). GUSB (glucuronidase b) was used as the normalization control.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…The oligonucleotide duplexes were cloned into the PGL3 control vector at the XbaI site in the correct and reverse (negative control) directions. Co-transfection and measurement of luciferase activity were performed as described previously (Luthra et al, 2008).…”

Section: Luciferase Reporter Assaymentioning

confidence: 99%

See 1 more Smart Citation

Hypoxia-regulated microRNA-210 modulates mitochondrial function and decreases ISCU and COX10 expression

et al. 2010

Self Cite

View full text Add to dashboard Cite

The mechanisms of compromised mitochondrial function under various pathological conditions, including hypoxia, remain largely unknown. Recent studies have shown that microRNA-210 (miR-210) is induced by hypoxia under the regulation of hypoxia-inducible factor-1a and has an important role in cell survival under hypoxic microenvironment. Hence, we hypothesized that miR-210 has a role in regulating mitochondrial metabolism and investigated miR-210 effects on mitochondrial function in cancer cell lines under normal and hypoxic conditions. Our results demonstrate that miR-210 decreases mitochondrial function and upregulates the glycolysis, thus make cancer cells more sensitive to glycolysis inhibitor. miR-210 can also activate the generation of reactive oxygen species (ROS). ISCU (iron-sulfur cluster scaffold homolog) and COX10 (cytochrome c oxidase assembly protein), two important factors of the mitochondria electron transport chain and the tricarboxylic acid cycle have been identified as potential targets of miR-210. The unique means by which miR-210 regulates mitochondrial function reveals an miRNAmediated link between microenvironmental stress, oxidative phosphorylation, ROS and iron homeostasis.

show abstract

“…MiRNAs expression profiling differs markedly between esophageal squamous cell carcinoma and esophageal adenocarcima, indicates that these two main forms of esophageal carcinoma have distinct etiologic and pathologic characteristics. The miRNAs which are highly expressed in tumor can work as tumor promoter by targeting and inhibiting diverse tumor suppressor genes that itself were involved in carcinogenesis of esophagus [112][113][114]. Apart from identifying different cancer-related miRNAs, efforts were made to recognize their target genes, messenger RNAs and receptors, which will result into their contribution in cancer treatment.…”

Section: Mirnas and Esophageal Carcinomamentioning

confidence: 99%

MiRNAs-based Gene Therapy on the Horizon: Novel and Effective Therapeutic Advancement

Noori–Daloii¹,

Nejatizadeh²

2011

Gene Therapy - Developments and Future Perspectives

View full text Add to dashboard Cite

MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers

Cited by 240 publications

References 28 publications

miR-196a2 polymorphisms and susceptibility to cancer: A meta-analysis involving 24,697 subjects

miR-196a2 polymorphisms and susceptibility to cancer: A meta-analysis involving 24,697 subjects

Hypoxia-regulated microRNA-210 modulates mitochondrial function and decreases ISCU and COX10 expression

MiRNAs-based Gene Therapy on the Horizon: Novel and Effective Therapeutic Advancement

Contact Info

Product

Resources

About