microRNA-199a may be involved in the pathogenesis of lupus nephritis via modulating the activation of NF-κB by targeting Klotho

Ye, Hong; Su, Bofeng; Ni, Hong-Bo; Li, Linlin; Chen, Xuduan; You, Xin; Zhang, Huidi

doi:10.1016/j.molimm.2018.10.003

Cited by 26 publications

(16 citation statements)

References 21 publications

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“…mTOR is the target gene of miR-199a [76], regulating cell survival, proliferation, migration, and apoptosis. In addition, miR-199a-3p increased TGF-β1-induced renal fibrosis via silencing of SOCS7 and STAT3 [41]. miR-199a has also been reported to be involved in inflammation via modulating the activation of NF-κB by targeting Klotho in lupus nephritis [42].…”

Section: Versatile Msc-evs-derived Mirnas Modulate Renal Injury and Hmentioning

confidence: 99%

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

Tsuji

Kitamura

Wada

2020

IJMS

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Mesenchymal stem cells (MSCs) have immunomodulatory and regenerative effects in many organs, including the kidney. Emerging evidence has shown that the trophic effects from MSCs are mainly mediated by the paracrine mechanism rather than the direct differentiation of MSCs into injured tissues. These secretomes from MSCs include cytokines, growth factors, chemokines and extracellular vesicles (EVs) containing microRNAs, mRNAs, and proteins. Many research studies have revealed that secretomes from MSCs have potential to ameliorate renal injury in renal disease models, including acute kidney injury and chronic kidney disease through a variety of mechanisms. These trophic mechanisms include immunomodulatory and regenerative effects. In addition, accumulating evidence has uncovered the specific factors and therapeutic mechanisms in MSC-derived EVs. In this article, we summarize the recent advances of immunomodulatory and regenerative effects of EVs from MSCs, especially focusing on the microRNAs.

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Section: Versatile Msc-evs-derived Mirnas Modulate Renal Injury and Hmentioning

confidence: 99%

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

Tsuji

Kitamura

Wada

2020

IJMS

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“…Hsa-miR199a may regulate the inhibitory kappa B (IκB) kinase β expression that is needed for NF-κB activation. Increased miR199a and decreased Klotho expression may lead to NFκB activation and accelerate the inflammatory process (Ye et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Data of quantitative RT-PCR were described by nominal CT value (normalized toU6), while fold changes were calculated using ΔΔCt. The expression rate of miR-199 was qualified by using the ΔΔCt (ΔΔCt = ΔC samples−ΔCtcontrol) (Ye et al, 2018).…”

Section: Cdna Synthesis and Quantitative Reverse Transcriptase Polymementioning

confidence: 99%

Evaluation of Micro-RNA199 in systemic lupus erythematosus patients with and without lupus nephritis

Elessawi

Radwan

El-Barbary

2020

Journal of Radiation Research and Applied Sciences

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Skin and kidney are commonly affected in systemic lupus erythematosus (SLE) with similar molecular mechanisms. Although clinical indicators of renal injury in SLE are fairly uncontroversial, few biomarkers are reliable. The role of Micro-RNAs (miRNAs) in lupus nephritis (LN) pathogenesis has been investigated. The aim of work is to evaluate miRNA199 expression in SLE Egyptian patients and their association with LN. This is a case-control study involving 98 SLE patients with and without LN (LN and non-LN groups), and 20 age-and sex-matched healthy controls were carried out to detect miRNA199 with quantitative Real-Time Polymerase chain reaction methods. The SLE disease activity index (SLEDAI) was assessed. The mean disease duration was 5.9 ± 3.4 and 6.4 ± 4.4 months in LN and non-LN, and SLEDAI was 11.98 ± 5.3 and 10.5 ± 3.8 in LN and non-LN. miRNA199 expression was significantly decreased in patient groups compared to controls (1.01 ± 0.03) (P = 0.0001) and in LN (0.41 ± 0.21) compared to non-LN group (0.55 ± 0.15) (P = 0.002). There was no correlation between miRNA199 expression and SLEDAI or other clinical and laboratory data of the patients. In conclusion, miRNA199 may play a role in SLE activity and helping in the early diagnosis of LN.

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“…MiR-214 also promotes kidney fibrosis in experimental animals (Denby et al, 2014), which may be linked to its ability to promote epithelial-to-mesenchymal transition (EMT) in tubular epithelial cells (Liu et al, 2018c). MiR-199a expression is downregulated in human renal cell carcinoma (He et al, 2015;Liu et al, 2018b) and deregulated in rodent genetic models of polycystic kidney disease (Dweep et al, 2013), in lupus nephritis (Ye et al, 2018), and in ischemia/reperfusion injury (Godwin et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The miR-199a/214 Cluster Controls Nephrogenesis and Vascularization in a Human Embryonic Stem Cell Model

et al. 2021

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Summary MicroRNAs (miRNAs) are gene expression regulators and they have been implicated in acquired kidney diseases and in renal development, mostly through animal studies. We hypothesized that the miR-199a/214 cluster regulates human kidney development. We detected its expression in human embryonic kidneys by in situ hybridization. To mechanistically study the cluster, we used 2D and 3D human embryonic stem cell (hESC) models of kidney development. After confirming expression in each model, we inhibited the miRNAs using lentivirally transduced miRNA sponges. This reduced the WT1 + metanephric mesenchyme domain in 2D cultures. Sponges did not prevent the formation of 3D kidney-like organoids. These organoids, however, contained dysmorphic glomeruli, downregulated WT1 , aberrant proximal tubules, and increased interstitial capillaries. Thus, the miR-199a/214 cluster fine-tunes differentiation of both metanephric mesenchymal-derived nephrons and kidney endothelia. While clinical implications require further study, it is noted that patients with heterozygous deletions encompassing this miRNA locus can have malformed kidneys.

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microRNA-199a may be involved in the pathogenesis of lupus nephritis via modulating the activation of NF-κB by targeting Klotho

Cited by 26 publications

References 21 publications

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

Immunomodulatory and Regenerative Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Renal Diseases

Evaluation of Micro-RNA199 in systemic lupus erythematosus patients with and without lupus nephritis

The miR-199a/214 Cluster Controls Nephrogenesis and Vascularization in a Human Embryonic Stem Cell Model

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