MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα

Wang, Yuanchuan; Yin, Xiaohong; Zhao, Long; Li, Shun; Duan, Jie; Kuang, Renzhao; Duan, Jun

doi:10.3892/etm.2017.4681

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…An increasing number of studies have found various miR-200b targets, including ZEB2, PKCα, and Bmi-1, which are expressed in gastric adenocarcinoma, pituitary tumors, and prostate cancer, respectively. 23 , 36 , 37 Therefore, we consider that other miR-200b-targeted proteins may play a role in the functional effects caused by miR-200b on HCC, and also lead to the upregulations of E-cadherin and Snail and vimentin.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein

Wang

Xie

Song

et al. 2018

Technol Cancer Res Treat

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HMGB3 belongs to the high-mobility group box subfamily and has been found to be overexpressed in gastric cancer. However, the expression and the role of HMGB3 in human hepatocellular carcinoma remain unknown. Here, we report that HMGB3, which is suppressed by miR-200b, contributes to cell proliferation and migration in human hepatocellular carcinoma. After analyzing The Cancer Genome Atlas data of 371 patients with hepatocellular carcinoma, we identified HMGB3 to be upregulated in human hepatocellular carcinoma tissue. Knockdown of HMGB3 in the hepatocellular carcinoma cell line suppressed cell proliferation and migration. TargetScan analysis showed miR-200b to be a possible regulator for HMGB3. Subsequent luciferase assays indicated that HMGB3 was a direct target of miR-200b. In addition, upregulation of miR-200b inhibited hepatocellular carcinoma cell growth and migration. HMGB3 overexpression or miR-200b downregulation was associated with poor prognosis. Our findings suggest HMGB3 may serve as an important oncoprotein whose expression is negatively regulated by miR-200b in hepatocellular carcinoma.

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Section: Discussionmentioning

confidence: 99%

Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein

Wang

Xie

Song

et al. 2018

Technol Cancer Res Treat

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“…miR-106b exerted promotive functions on PitNET cell proliferation and invasion via targeting tumor suppressor PTEN 37. By contrast, miR-15a/16,38 miR-183,39 miR-148b,40 miR-200b,41 miR-524-5p42 and etc. could repress PitNET cell proliferation, invasion and migration by target various oncogenic genes.…”

Section: Discussionmentioning

confidence: 99%

<p>LncRNA XIST depletion prevents cancer progression in invasive pituitary neuroendocrine tumor by inhibiting bFGF via upregulation of microRNA-424-5p</p>

Zhou¹,

Li²,

Du³

et al. 2019

OTT

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Background Long noncoding RNAs (lncRNAs) are vital mediators in human cancers including pituitary neuroendocrine tumor (PitNET) and could function as competing endogenous RNAs (ceRNAs) of microRNAs (miRNAs). The main objective of this study is to identify effect of lncRNA X-inactive specific transcript (XIST) and microRNA-424-5p (miR-424-5p) on PitNET. Methods Microarray analysis was employed to identify the PitNET-related differentially expressed lncRNAs. PitNET tissues, including both invasive and non-invasive subtypes in parallel with normal pituitary tissues were collected for the determination of the expression of XIST, miR-424-5p and basic fibroblast growth factor (bFGF) and the interaction among them. Subsequently, the expression of XIST, miR-424-5p and bFGF in PitNET cells was altered to elucidate their biological significance in the aspects of proliferation, migration, invasion, and the apoptosis. Results Both XIST and bFGF exhibited high expression, but miR-424-5p had a low expression in invasive PitNET tissues as compared to non-invasive PitNET normal pituitary tissues. Additionally, XIST competitively bound to miR-424-5p to elevate the expression of bFGF. Furthermore, depleted XIST or bFGF, or elevated miR-424-5p was revealed to suppress the proliferation, migration, invasion, and promote cell cycle arrest and apoptosis of invasive PitNET cells. miR-424-5p repressed the proliferation, migration, invasion of invasive PitNET cells by targeting bFGF. Conclusion In conclusion, the fundamental findings of the present study suggested that the functional suppression of XIST downregulated bFGF to inhibit the development of PitNET by increasing miR-424-5p expression, proposing XIST as a novel therapeutic target for PitNET.

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“…Chen et al found that miR-200b involved in the physiological process of development, growth, and hormone secretion of prolactinoma [ 57 ]. In another study, the expression of miR-200b and protein kinase Cα (PKCα) in pituitary tumors was investigated to determine whether miR-200b may inhibit proliferation and invasion of pituitary tumor cells [ 58 ]. The involvement of miR-429 in humans has been tied to tumorigenesis, particularly in the epithelial-to-mesenchymal transition (EMT) [ 59 ].…”

Section: Circulating Mirnas and Pamentioning

confidence: 99%

MiRNAs as Noninvasive Biomarkers and Therapeutic Agents of Pituitary Adenomas

Beylerli

Beeraka

Gareev

et al. 2020

IJMS

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Pituitary adenoma (PA) accounts for 10–15% of all intracranial neoplasms. Even though most pituitary adenomas are benign, it is known that almost 35% of them exhibit an aggressive clinical course, including rapid proliferative activity and invasion of neighboring tissues. MicroRNAs (miRNAs) are short single-stranded RNA molecules that can influence post-transcriptional regulation by controlling target genes. Based on research data on miRNAs over the past 20 years, more than 60% of genes encoding human proteins are regulated by miRNAs, which ultimately control basic cellular mechanisms, including cell proliferation, differentiation, and apoptosis. Dysregulation of miRNAs has been observed in a number of diseases, especially tumors like PA. A majority of miRNAs are expressed within the cells themselves. However, the circulating miRNAs can be detected in several biological fluids of the human body. The identification of circulating miRNAs as new molecular markers may increase the ability to detect a tumor, predict the course of a disease, plan to choose suitable treatment, and diagnose at the earliest signs of impending neoplastic transformation. Therapy of PAs with aggressive behavior is a complex task. When surgery and chemotherapy fail, radiotherapy becomes the treatment of choice against PAs. Therefore, the possibility of implementing circulating miRNAs as innovative diagnostic and therapeutic agents for PA is one of the main exciting ideas.

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MicroRNA-200b inhibits pituitary tumor cell proliferation and invasion by targeting PKCα

Cited by 8 publications

References 45 publications

Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein

Upregulation of miR-200b Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration by Targeting HMGB3 Protein

<p>LncRNA XIST depletion prevents cancer progression in invasive pituitary neuroendocrine tumor by inhibiting bFGF via upregulation of microRNA-424-5p</p>

MiRNAs as Noninvasive Biomarkers and Therapeutic Agents of Pituitary Adenomas

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