MicroRNA-210 Plays a Critical Role in the Angiogenic Effect of Isoprenaline on Human Umbilical Vein Endothelial Cells via Regulation of Noncoding RNAs

Abstract

Background:β-adrenoceptors play a crucial regulatory role in blood vessel endothelial cells. Isoprenaline (ISO, a β-adrenergic agonist) has been reported to promote angiogenesis through upregulation of vascular endothelial growth factor (VEGF) expression; however, the underlying mechanism remains to be investigated. It is widely accepted that certain noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), can regulate endothelial cell behavior, including their involvement in angiogenesis. Therefore, we aimed to investigate whether noncoding RNAs participate in ISO-mediated angiogenesis using human umbilical vein endothelial cells (HUVECs).Methods:We evaluated VEGF-A messenger RNA (mRNA) and protein levels in ISO-treated HUVECs by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. To establish whether noncoding RNAs are associated with ISO-mediated angiogenesis, we measured expression of the miRNAs miR-210, miR-21, and miR-1, as well as that of the lncRNAs growth arrest-specific transcript 5 (GAS5), maternally expressed 3 (MEG3), and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in HUVECs exposed to ISO. Furthermore, to ascertain its importance in ISO-mediated angiogenesis, we constructed the HUVECs with overexpressing miR-210 and detected the subsequent expression of VEGF-A and noncoding RNAs. All statistical analyses were performed using SPSS 16.0 software. Intergroup comparisons were carried out by one-way analysis of variance.Results:VEGF-A mRNA levels were elevated in the ISO group (1.57 ± 0.09) compared to those in the control group (P < 0.01). Moreover, concentrations of VEGF-A in culture supernatants significantly differed between the control (113.00 ± 19.21 pg/ml) and ISO groups (287.00 ± 20.27 pg/ml; P < 0.01). Expression of miR-1, miR-21, and miR-210 was higher (3.89 ± 0.44, 2.87 ± 087, and 3.33 ± 1.31, respectively) in ISO-treated cells than that in controls (P < 0.01), whereas that of GAS5 and MEG3 (0.22 ± 0.10 and 0.58 ± 0.16, respectively) was lower as a result of ISO administration (P < 0.05). There was no significant difference in the expression of MALAT1 between the groups. Interestingly, miR-210 overexpression heightened the levels of VEGF-A and miR-21 (5.87 ± 1.24 and 2.74 ± 1.15, respectively; P < 0.01) and reduced those of GAS5 and MEG3 (0.19 ± 0.01 and 0.09 ± 0.05, respectively; P < 0.01).Conclusions:ISO-mediated angiogenesis was associated with altered expression of miR-210, miR-21, and the lncRNAs GAS5 and MEG3. The effects of miR-210 on the expression of VEGF-A and noncoding RNAs were similar to those of ISO, indicating that it might play an important role in ISO-mediated angiogenesis.

MicroRNA‐210 regulates human trophoblast cell line HTR‐8/SVneo function by attenuating Notch1 expression: Implications for the role of microRNA‐210 in pre‐eclampsia

“…A previous study demonstrated that overexpression of miR‐210 reduced maternally expressed 3 (MEG3) expression (Y.‐Y. Yan et al, ). MEG3 was reported to be downregulated in 80% of preeclampstic placentas (Song et al ().…”

The regulatory role of microRNAs in angiogenesis‐related diseases

“…Further research indicated that miR‐210 facilitates angiogenesis through negatively regulating the target gene, ephrin A3, which is an important member of the ephrin angiogenesis regulatory gene family 51, 91, 92. Moreover, a study revealed that isoprenaline enhances the levels of miR‐210, miR‐21 and miR‐1 and decreases those of the lncRNAs maternally expressed 3 (MEG3) and growth arrest‑specific transcript 5 (GAS5), thereby improving angiogenesis 93. The inhibition of miR‐210 increases tumour cell apoptosis and autophagy and represses angiogenesis 94.…”

Long Non-coding RNA MEG3 Attenuates the Angiotensin II-Induced Injury of Human Umbilical Vein Endothelial Cells by Interacting With p53

“…Furthermore, knockdown of MALAT1 in vivo inhibited proliferation of endothelial cells and reduced neonatal retina vascularization (Michalik et al, 2014). In addition, Yan Y.Y. et al (2016) have found arrest-specific transcript 5 (GAS5) and Meg3 were significantly downregulated in isoprenaline treated HUVECs.…”

“…It is part of the DLK1-Meg3 locus located on human chromosome 14q32 (Miyoshi et al, 2000) and was believed to be associated with ECs’ injury (Zhang et al, 2003). Its expression level was also found to be related to downregulation in isoprenaline treated HUVECs (Yan Y.Y. et al, 2016).…”