MicroRNA‐214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells

Abstract

SummaryMicroRNAs are a group of endogenously expressed, singlestranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3 0 UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.

miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2

“…Overexpression of miR-214 in HeLa cells also significantly inhibited cell proliferation. In addition, HeLa cells that stably overexpressed miR-214 downregulated the expression of MEK3 and JNK1 at both the mRNA and protein levels by targeting the 3'UTRs of these genes (22). Finally, it has been reported that miR-34a inhibits invasiveness through regulation of the Notch pathway and its downstream matrix degrading enzyme in cervical cancer (23).…”

MicroRNA-214 Regulates the Acquired Resistance to Gefitinib via the PTEN/AKT Pathway in EGFR-mutant Cell Lines

“…It has been documented to be aberrantly expressed in many tumors including breast, cervical, hepablastoma, pancreatic and lung cancer (Yanaihara et al, 2006;Magrelli et al, 2009;Yang et al, 2009;Zhang et al, 2010Derfoul et al, 2011. Accumulating evidence indicated that miR-214 functions either as onco-gene or tumor suppressor gene in different tumors.…”

miR-126 Suppresses the Proliferation of Cervical Cancer Cells and Alters Cell Sensitivity to the Chemotherapeutic Drug Bleomycin

“…Previous studies have indicated that microRNAs (miRNAs) act as crucial modulators in cancer progression by targeting mRNAs through cleavage or transcriptional repression (Bartel, 2004). Various miRNAs have been shown to exhibit differential expression between cervical cancer and normal cervical tissues (Wang et al, 2008), and several reports have demonstrated the roles of miRNAs in the proliferation (Yang et al, 2009), apoptosis (Li et al, 2011), and prognosis (Hu et al, 2010) of cervical cancer. However, the role of miRNAs in mediating sensitivity of cells to chemotherapeutic drugs has not been explored in cervical cancer.…”